Journal of Animal Science and Technology

Korean Society of Animal Sciences and Technology (한국축산학회)
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Isolation and Identification of Hydrolytic Enzyme-producing Bacteria from Spent Mushroom Substrate

버섯부산물유래 가수분해효소분비 박테리아의 분리 및 동정

  • Kim, Young-Il (Animal Science, School of Life Resource and Environmental Sciences College of Natural Sciences, Konkuk University) ;
  • Jeong, Se-Hyung (Animal Science, School of Life Resource and Environmental Sciences College of Natural Sciences, Konkuk University) ;
  • Seok, Joon-Sang (Animal Science, School of Life Resource and Environmental Sciences College of Natural Sciences, Konkuk University) ;
  • Yang, Si-Yong (Animal Science, School of Life Resource and Environmental Sciences College of Natural Sciences, Konkuk University) ;
  • Huh, Jung-Won (Animal Science, School of Life Resource and Environmental Sciences College of Natural Sciences, Konkuk University) ;
  • Kwak, Wan-Sup (Animal Science, School of Life Resource and Environmental Sciences College of Natural Sciences, Konkuk University)
  • 김영일 (건국대학교 자연과학대학 생명자원환경과학부 축산학전공) ;
  • 정세형 (건국대학교 자연과학대학 생명자원환경과학부 축산학전공) ;
  • 석준상 (건국대학교 자연과학대학 생명자원환경과학부 축산학전공) ;
  • 양시용 (건국대학교 자연과학대학 생명자원환경과학부 축산학전공) ;
  • 허정원 (건국대학교 자연과학대학 생명자원환경과학부 축산학전공) ;
  • 곽완섭 (건국대학교 자연과학대학 생명자원환경과학부 축산학전공)
  • Published : 2008.10.01
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Abstract

This study was conducted to isolate and identify xylanase- and cellulase-producing thermophilic bacteria from stacked spent mushroom substrates and to determine the optimal medium conditions for their growth. Bacteria with the highest xylanase and CMCase activities were strain 3 and 201-7. Both of them were identified as Bacillus spp. and named Bacillus subtilis KU3 and Bacillus subtilis KU201-7. The optimal medium condition of Bacillus subtilis KU3 was obtained when 3%(w/v) of yeast extract and 1%(w/v) of maltose were used as nitrogen and carbon sources, respectively. That of Bacillus subtilis KU201-7 was obtained when 0.5%(w/v) of yeast extract and 0.5%(w/v) of CMC were used as nitrogen and carbon sources, respectively.

본 연구는 톱밥주원료 버섯부산물의 효과적 사료화를 위한 균주개발을 목적으로 버섯부산물로부터 섬유소분해력이 높은 고온성 균주를 분리 동정하고 균주생산을 위한 배지의 최적화 조건을 도출하고자 실시하였다. Xylanase와 CMCase 활력이 상대적으로 우수한 것으로 선발된 고온성 균은 3, 201-7번으로서 동정한 결과 Bacillus spp.에 속하는 균주로 B. subtilis KU3, B. subtilis KU201-7로 각각 명명하였다. 균주생산을 위한 최적 액상배양조건에 있어서 B. subtilis KU3은 질소원으로 yeast extract 3%(w/v)에서, 탄소원으로 maltose 1%(w/v)에서 성장이 가장 좋았다. B. subtilis KU201-7은 질소원으로 yeast extract 0.5%(w/v)에서, 탄소원으로 CMC 0.5%(w/v)에서 성장이 가장 좋았다.

Keywords

References

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