Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Artemisia capillaris and Paecilomyces japonica Stimulate Lipid Metabolism and Reduce Hepatotoxicity Induced by Carbon Tetrachloride in Rats

사염화탄소를 투여한 흰쥐에서 인진쑥 및 동충하초의 지질대사촉진 및 간독성저하효과

  • 김영섭 (한림대학교 식품영양학과) ;
  • 유윤숙 (한림대학교 식품영양학과) ;
  • 한은경 (한림대학교 식품영양학과) ;
  • 강일준 (한림대학교 식품영양학과) ;
  • 정차권 (한림대학교 식품영양학과)
  • Published : 2008.05.31
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Abstract

The aim of this study was to identify the inhibitory effect of hepatic toxicity and liver lipid metabolism after the administration of Artemisia capillaris and Paecilomyces japonica. SD rats were divided into $CCI_4$ treated group with subgroups of 6% Artemisia capillaries (6A), 4% Artemisia capillaris＋2% Paecilomyces japonica (4A2P), 3% Artemisia capillaris＋3% Paecilomyces japonica (3A3P), 2% Artemisia capillaris＋4% Paecilomyces japonica (2A4P) and 6% Paecilomyces japonica (6P). In this study we also intended to verify the optimum ratio of Artemisia capillaris and Paecilomyces japonica which can reduce hepatotoxicity. Artemisia capillaris and Paecilomyces japonica reduced cholesterol and triglyceride levels which were increased by the treatment of $CCI_4$. HDL-cholesterol level was the most enhanced in the group of 4A2P. On the other hand, athrogenic index (AI) was reduced statistically (p<0.05). When the ratio of Artemisia capillaris and Paecilomyces japonica was 2:1, the improvement of rat serum and liver lipid metabolism and the alleviation of hepatic damage induced by $CCI_4$ were shown to be the most effective in this study. It is considered that the symptoms of severe chemically induced hepatotoxicity could be lessened by Artemisia capillaris and Paecilomyces japonica administration.

본 연구는 $CCI_4$를 투여한 흰쥐에서 인진쑥과 동충하초를 서로 다른 혼합비율로 투여했을 때 간 손상에 대한 생리활성 기능을 조사하기 위해 혈청 및 지질대사에 미치는 영향을 측정하였다. 사염화탄소의 투여는 간독성을 나타내 간의 무게 및 ALT, AST 수준을 증가시켰으며 이는 인진쑥과 동충하초의 투여에 의해 개선되며 인진쑥과 동충하초를 병행 투여 시 더욱 효과적인 것으로 나타났다. 사염화탄소 투여로 인해 증가된 혈청 콜레스테롤과 중성지방은 인진쑥, 동충하초의 투여로 저하되었으며 혈청 중성지방은 인진쑥과 동충하초를 동량으로 투여한 군에서 유의적으로 낮게 나타났고, 혈청 콜레스테롤은 전체 인진쑥, 동충하초 투여군에서 모두 유의적으로 감소되었으며 인진쑥과 동충하초를 병행 투여하는 경우 더 효과적인 것으로 나타났다. 또한 동맥경화지수는 사염화탄소의 투여 시 정상식이군보다 유의적으로 증가되었으나 인진쑥, 동충하초의 투여로 감소되었으며 4% 인진쑥과 2% 동충하초 병행 투여가 동맥경화지수를 낮추는데 가장 효율적인 것으로 사료된다. 사염화탄소를 투여한 흰쥐의 간장 내 총 지질과 총 콜레스테롤 및 중성지방의 함량은 정상군보다 증가하였다. 인진쑥, 동충하초의 투여는 사염화 탄소 투여로 높아진 중성지방과 콜레스테롤을 감소하는 효과를 보였으며 4% 인진쑥과 2% 동충하초를 병행 투여 시가장 효과적인 것으로 나타났다. 본 연구 결과에 의하면 인진쑥과 동충하초의 투여는 사염화탄소로 유발된 간 독성을 저하시키며 혈청과 간지질 대사를 개선하는 효과가 있는 것으로 사료되며 간 기능 개선과 지질대사 개선효과를 가지고있는 인진쑥과 동충하초를 단독으로 투여하는 것보다 병행 투여하는 것이 더 효과적이며 그 혼합비율이 4% 인진쑥과 2% 동충하초에서 가장 효율적으로 나타났으나 혼합비율에 대해서는 앞으로 지속적인 연구가 필요하다고 사료된다.

Keywords

References

  1. Lee HJ, Hwang EH, Yu HH, Song IS, Kim CM, Kim MC, Hong JH, Kim DS, Han SB, Kang KJ, Lee EJ, Chung HW. 2002. The analysis of nutrients in Artemisia capillaris Thunberg. J Korean Soc Food Sci Nutr 31: 361-366 https://doi.org/10.3746/jkfn.2002.31.3.361
  2. Kim JO, Kim YS, Lee JH, Kim MN, Lee SH, Park GY. 1992. Antimutagenic effect of the major volatile compounds identified from mugwort (Artemisia asictica Nakai) leaves. J Kor Soc Food Nutr 21: 308-313
  3. Lee JS. 1996. Studies on the antimicrobial effect of mugwort (Artemisia asictica Nakai) leaves. MS Thesis. The Catholic University, Korea
  4. Lee GD, Kim JS, Bae JO, Yoon HS. 1992. Antioxidative effectiveness of water extract and ether extract in wormwood (Artemisia montana Pampan). J Korean Soc Food Nutr 21: 17-22
  5. Xu QA, Mori H, Sakamoto O, Uesugi Y, Koda A. 1989. Immunological mechanisms of antitumor activity of some kinds of crude drugs on tumor necrosis factor production. Int J Immunopharmacol 11: 607-613 https://doi.org/10.1016/0192-0561(89)90145-8
  6. JianZhe Y, Xiaoloan M, Yichen Z, Huaan W. 1989. Icons of Medical Fungi from China. Science Press, China. p 575-585
  7. Zhu JS, Halpern GM, Jones K. 1998. The scientific rediscovery of an ancient Chinese herbal regmen, Cordyceps sinensis Part I. J Altern Complement Med 4: 289-303 https://doi.org/10.1089/acm.1998.4.3-289
  8. Zhu JS, Halpern GM, Jones K. 1998. The scientific rediscovery of an ancient Chinese herbal regmen, Cordyceps sinensis Part II. J Altern Complement Med 4: 2429-2457
  9. Lu LY, Yin L, Wang YJ, Xiao LC. 1997. Studies on pharmacological activities of cultivated Cordycepssinensis. Phytother Res 11: 237-239 https://doi.org/10.1002/(SICI)1099-1573(199705)11:3<237::AID-PTR49>3.0.CO;2-2
  10. Manabe N, Sugimoto M, Azuma Y, Taketomo N, Yamashita A, Tsuboi H, Tsunoo A, Kinjo N, Nian-Lai H, Miyamoto H. 1996. Effects of the mycelial extract of cultured Cordyceps sinensis on in vivo hepatic energy metabolism in the mouse. Jpn J Pharmacol 70: 85-88 https://doi.org/10.1254/jjp.70.85
  11. Guan YJ, Hu Z, Hou M. 1992. Effect of Cordyceps sinensis on T-lymphocyte subsets in chronic renal failure. Zhongguo Zhong Xiyijiehe Zazhi 12: 338-339
  12. Zhu JL, Liu C. 1992. Modulating effects of extract from Semen Persicae and cultivated Cordyceps hyphae on immuno- dysfunction of inpatients with post hepatic cirrhosis. Zhongguo Zhong Xiyijiehe Zazhi 12: 207-209
  13. Kuo YC, Lin CY, Tsai WJ, Wu CL, Chen CF, Shiao MS. 1994. Growth inhibitors against tumor cells in Cordyceps sinensis other than cordycepin and polysaccharides. Cancer Invest 12: 611-615 https://doi.org/10.3109/07357909409023046
  14. Balon TW, Jasman AP, Zhu JS. 2002. A fermentation product of Cordyceps sinensis increases whole-body insulin sensitivity in rats. J Altern Complement Med 8: 315-323 https://doi.org/10.1089/10755530260128005
  15. Anita KC, James RT. 1989. Interaction of hypoxia and carbon tetrachloride toxicity in hepatocyte monolayers. Exp Mol Pathol 50: 183-192 https://doi.org/10.1016/0014-4800(89)90029-4
  16. Thomas BD, Mariani ME. 1989. Comparison of metabolic effects of carbon tetrachloride and 1,2-dichloroethane added in vitro to slice of rat liver. Toxicol In Vitro 3: 159-168 https://doi.org/10.1016/0887-2333(89)90025-8
  17. Nam SM, Ham SS, Oh DH, Kang IJ, Lee SY, Chung CK. 1998. Effects of Artemisia iwayomogi Kitamura ethanol extracts on lowering serum and liver lipids in rats. J Korean Soc Food Sci Nutr 27: 338-343
  18. Lee HJ, Hwang EH. 2002. Effects of Artemisia capillaris Thunberg on the plasma and liver lipid metabolism in rats. Korean J Nutrition 35: 421-430
  19. Lim SS, Lee JH. 1997. Effect of Artemisia Princeps var Orientalis and Circium japonicum var Ussuriense on serum lipid of hyperlipidemic rat. Korean J Nutrition 30: 12-18
  20. Yoshinobu K, Yoriko S, Chohachi K, Hiroshi H, Ichio H, Yukiko Y. 1982. Application of carbon-tetrachloride-induced liver lesion in mice for screening of liver protective crude drugs. Shoyakugaku Zasshi 36: 238-244
  21. Cho MK, Choe SY, Hong SM, Kim BS. 1998. Effects of scoparone on liver function. J Korean Soc Food Sci Nutr 27: 344-349
  22. Lee SG. 2005. The therapeutic effect of Artemisia capillaris extract on hepatic damage induced by carbon tetrachloride in rats. J Vet Clin 22: 206-213
  23. Koh JB. 2002. The effect of Cordyceps militaris on lipid metabolism, protein levels and enzyme activities in rats fed high fat diet. Korean J Nutrition 35: 414-420
  24. Kwon SH, Woo HJ, Han DS, Kim MK. 2001. Effect of dried powders and water extracts of Paecilomyces tenuipes and Cordyceps militaris on lipid metabolism, antioxidative capacity and immune status in rats. Korean J Nutrition 34: 271-284
  25. Koo BS, Lee MS. 2004. Effects of Cordyceps militaris extract powder on plasma lipids and glucose in rats. Korean J Food Culture 19: 217-222
  26. Folch J, Less M, Sloane-Stanley GH. 1957. A smaple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 226: 497-509
  27. Bligh EG, Dyer WJ. 1959. A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37: 911-917 https://doi.org/10.1139/o59-099
  28. Jeon JR, Park JR. 2002. Effect of Eucommia ulmoides leaf water extract on hepatotoxicity of carbon tetrachlorideinduced rats. J Korean Soc Food Sci Nutr 31: 124-130 https://doi.org/10.3746/jkfn.2002.31.1.124
  29. Lee SB, Cho TS, Yoon KW, Lee JC, Lee SM, Shim SB. 1998. A study on hepatoprotective effect of PS-1 from Artemisia iwayomogi. J Appl Pharmacol 6: 119-129
  30. Koh JB. 2001. Effect of fruiting body of Cordyceps militaris on growth, lipid and protein metabolism, and enzyme activities in male rats. Korean J Nutrition 34: 741-747
  31. Koh JB, Choi MA. 2001. Effect of Cordyceps militaris on lipid metabolism in rats fed cholesterol diet. Korean J Nutrition 34: 265-270
  32. Koh JB, Choi MA. 2003. Effect of Paecilomyes japonica on lipid metabolism in rats fed high fat diet. J Korean Soc Food Sci Nutr 32: 238-243 https://doi.org/10.3746/jkfn.2003.32.2.238
  33. Kim OK. 2002. Protective effects of Houttuynia cordata Thunb on carbon tetrachloride-induced hepatotoxicity in rats. Kor J Pharmaco 33: 324-331
  34. Kim OK. 2004. The effects of Sedurm sarmentosum Bunge extract using super critical carbon dioxide on lipid metabolism, lipid peroxidation and antioxidation in carbon tetrachloride induced hepatotoxicity in rats. J Korean Oil Chem 21: 204-213
  35. Chen TY, Lu CF, Hu QY, Wang SX, Tan H. 2004. Protec Protective effect of Yinchenhao Tang on hepatocytes of rats with experimental hepatic fibrosis and ultrastructural findings. Heilongjiang Med Pham 27: 11-12
  36. Lee SB, Jeong C, Jeong SH, Lee SM, Shim SB, Cho TS. 1997. Hepatoprotective effects of extracts from Artemisia iwayomogi. J Appl Pharmacol 5: 194-201

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