Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Identification of Chemical Structure and Free Radical Scavenging Activity of Diphlorethohydroxycarmalol Isolated from a Brown Alga, Ishige okamurae

  • Heo, Soo-Jin (Faculty of Applied Marine Science, Cheju National University) ;
  • Kim, Jong-Pyung (Functional Metabolites Research Center, KRIBB) ;
  • Jung, Won-Kyo (Department of Marine Life Science, Chosun University) ;
  • Lee, Nam-Ho (Department of Chemistry, Cheju National University) ;
  • Kang, Hahk-Soo (Functional Metabolites Research Center, KRIBB) ;
  • Jun, Eun-Mi (Functional Metabolites Research Center, KRIBB) ;
  • Park, Soon-Hye (Functional Metabolites Research Center, KRIBB) ;
  • Kang, Sung-Myung (Faculty of Applied Marine Science, Cheju National University) ;
  • Lee, Young-Jae (Department of Veterinary Science, Cheju National University) ;
  • Park, Pyo-Jam (Department of Biotechnology, Konkuk University) ;
  • Jeon, You-Jin (Faculty of Applied Marine Science, Cheju National University)
  • Published : 2008.04.30
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Abstract

To obtain a natural antioxidant from a marine biomass, this study investigated the antioxidative activity of methanolic extracts from the marine brown alga, Ishige okamurae collected off Jeju Island. A potent free radical scavenging activity was detected in the ethyl acetate fraction containing polyphenolic compounds, and the potent antioxidant elucidated as a kind of phlorotannin, diphlorethohydroxycarmalol, by NMR and mass spectroscopic data. The free radical scavenging activities of the diphlorethohydroxycarmalol were investigated in relation to 1,1-diphenyl-2-picrylhydrazyl (DPPH), alkyl, and hydroxyl radicals using an electron spin resonance (ESR) system. The diphlorethohydroxycarmalol was found to scavenge DPPH ($IC_{50}=3.41{\mu}M$) and alkyl ($IC_{50}=4.92{\mu}M$) radicals more effectively than the commercial antioxidant, ascorbic acid. Therefore, these results present diphlorethohydroxycarmalol as a new phlorotannin with a potent antioxidative activity that could be useful in cosmetics, foods, and pharmaceuticals.

Keywords

References

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