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Antioxidant Activity of the Ethanol Extract from Cooking Drips of Thunnus thynnus by Gamma Irradiation

참치 자숙액의 항산화 활성에 감마선 조사가 미치는 영향

  • Lee, Hee-Sub (Team for Radiation Food Science and Biotechnology, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Kim, Hyun-Joo (Team for Radiation Food Science and Biotechnology, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Choi, Jong-Il (Team for Radiation Food Science and Biotechnology, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Kim, Jae-Hun (Team for Radiation Food Science and Biotechnology, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Kim, Jin-Gyu (Team for Radiation Food Science and Biotechnology, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Chun, Byung-Soo (Faculty of Food Science and Biotechnology, Pukyong National University) ;
  • Ahn, Dong-Hyun (Faculty of Food Science and Biotechnology, Pukyong National University) ;
  • Chung, Young-Jin (Dept. of Food and Nutrition, Chungnam National University) ;
  • Kim, Yeung-Ji (Division of Food Beverage and Culinary Art, Yeungnam College of Science and Technology) ;
  • Byun, Myung-Woo (Team for Radiation Food Science and Biotechnology, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Lee, Ju-Woon (Team for Radiation Food Science and Biotechnology, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute)
  • 이희섭 (한국원자력연구원 정읍방사선과학연구소 방사선식품생명공학연구실) ;
  • 김현주 (한국원자력연구원 정읍방사선과학연구소 방사선식품생명공학연구실) ;
  • 최종일 (한국원자력연구원 정읍방사선과학연구소 방사선식품생명공학연구실) ;
  • 김재훈 (한국원자력연구원 정읍방사선과학연구소 방사선식품생명공학연구실) ;
  • 김진규 (한국원자력연구원 정읍방사선과학연구소 방사선식품생명공학연구실) ;
  • 전병수 (부경대학교 식품생명공학부) ;
  • 안동현 (부경대학교 식품생명공학부) ;
  • 정영진 (충남대학교 식품영양학과) ;
  • 김영지 (영남이공대학 식품조리계열) ;
  • 변명우 (한국원자력연구원 정읍방사선과학연구소 방사선식품생명공학연구실) ;
  • 이주운 (한국원자력연구원 정읍방사선과학연구소 방사선식품생명공학연구실)
  • Published : 2008.06.30

Abstract

This study was conducted to examine the effect of a gamma irradiation on the antioxidant activity of ethanol extract from cooking drips of Thunnus thynnus (ECT). The electron donating ability against 1,1-diphenyl- 2-picryl-hydrazyl (DPPH), authentic hydroxyl radical scavenging activity by Ultra Weak Luminescence Analyzer, oxidative protection factors by Racimat method, ferric reducing antioxidant power (FRAP), and ${\beta}$-carotene bleaching activity were measured as indices of antioxidant activity. All of the results shown that irradiation increased the antioxidant activity of ECT. These results suggested that ECT could be used as an antioxidative component in food and gamma irradiation could enhance these antioxidative properties.

수산 가공 폐기물중 하나인 참치 자숙액을 이용하여 천연기능성 소재 개발을 하기 위해 참치 자숙액 에탄올 추출물에 감마선 조사(0, 50 kGy)를 하여 전자공여능을 확인하고, FRAP, UWLA, Rancimat 등을 사용하여 항산화 활성 변화에 관하여 알아보았다. 감마선 조사한 참치 자숙액의 전자공여능 및 hydroxyl 라디칼 소거능 측정 결과 감마선 조사에 의해 각각 16.17% 및 10.38% 증가하였고, 산화안정지수 및 FRAP value 또한 각각 23% 및 26% 증가하였다. ${\beta}$-carotene bleaching assay를 이용하여 항산화지수를 측정한 결과 감마선 조사에 의해 항산화지수가 증가하는 경향을 보였다. 이러한 결과로 미루어 볼 때 참치 자숙액 자체가 가지고 있는 항산화 활성 및 산화안정성을 감마선 조사 기술을 사용하여 증진시켜 식품 및 공중보건산물의 첨가제로서의 이용이 가능할 것이라 사료된다.

Keywords

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