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Occurrence and Biovar Classification of Bacterial Wilt Caused by Ralstonia solanacearum in Eggplant (Solanum melongena)

가지의 Ralstonia solanacearum에 의한 풋마름병 발생과 생리형의 분화

  • 임양숙 (경상북도농업기술원 원예연구과) ;
  • 이문중 (경상북도농업기술원 원예연구과) ;
  • 정종도 (경상북도농업기술원 원예연구과) ;
  • 류영현 (경상북도농업기술원 원예연구과) ;
  • 김병수 (경북대학교 원예과)
  • Published : 2008.04.01

Abstract

Batcterial wilt caused by Ralstonia solanacearum is one of important and widespread diseases worldwide as well as in Korea. Bacterial wilt disease caused by R. solanacearum has been reported mainly in solanaceous crops including eggplant (Solanum melongena), tomato (Solanum lycopersicum), potato (S. tuberosum), and pepper (Capsicum annuum). A total of 48 strains of R. solanacearum from eggplant were collected during 2005 and 2006. They were confirmed as R. solanacearum by PCR amplification with primer pair flipcF/flipcR resulting in production of 470-bp DNA fragment. The 15 isolates exhibited pathogenicity on eggplant and tomato, but less virulent on pepper than other species. The biovar of collected isolates, which have been reported of five types worldwide, were classified as biovars 3 and 4 by physiological test. Biovar 4 was the dormant type without pathogenicity on eggplant rootstock, whereas biovar 3 had pathogenicity on eggplant rootstocks that is resistant to R. solanacearum, indicating necessity of breeding new rootstock with resistance to R. solanacearum biovar 3

Ralstonia solanacearum에 의한 풋마름병은 세계적으로 치명적인 병해중의 하나이며 우리나라에서는 가지, 토마토, 감자, 고추 등 가지과 작물에 발병이 보고되어 있다. 2005년${\sim}$2006년 2년 동안 가지 주재배단지의 풋마름병 발생 포장에서 48개의 균주를 수집하여 분리 배양하였으며 R. solanacearum의 특이적인 flipcF/flipcR primer를 이용하여 PCR을 실시한 결과 470-bp의 풋마름병 특이적 밴드가 관찰되어 R. solanacearum으로 동정하였다. 분리 동정된 15균주로 각 기주별 병원성을 검정하였을 때 가지, 토마토에 병원성을 나타내었으나 고추는 저항성을 나타내었다. 가지 풋마름병원균의 biovar분화는 biovar 3, 4로 동정되었으며 특히 biovar 3는 가지 풋마름병 저항성 대목에 다소 강한 병원성을 나타내어 저항성대목의 육종이 필요한 것으로 판단된다.

Keywords

References

  1. Hayward, A. C. 1964. Characteristics of Pseudomonas solanacearum. J. Appl. Bacteriol. 27: 265-277 https://doi.org/10.1111/j.1365-2672.1964.tb04912.x
  2. Hayward, A. C. 1991. Biology and epidemiology of bacterial wilt caused by Pseudomonas solanacearum. Annu. Rev. Phytopathol. 29: 65-87 https://doi.org/10.1146/annurev.py.29.090191.000433
  3. Jeong, Y., Kim, J., Kang, Y., Lee, S. and Hwang, I. 2007. Genetic diversity and distribution of Korea isolates of Ralstonia solanacearum. Plant Dis. 91: 1277-1287 https://doi.org/10.1094/PDIS-91-10-1277
  4. Kazutaka, Y., Yasuaki, M., Youich, K. and Shinji, K. 2000. Distribution of a baterial group of Ralstonia solanacearum isolated from eggplants in Kochi prefecture and its control by using resistant rootstock. Bull. Kochi. Agri. Res. Cent. 9: 9-16
  5. Kelman, A. 1954. The Relationship of pathogenicity in Pseudomonas solanacearum to colony appearance on a tetrazolium medium. Phytopathology 44: 693-695
  6. Mitsuo, H. and Kenichi, T. 1999. Phenotypic characteristics and cluster analysis of Japanese and reference strains of Ralstonia solanacearum. Ann. Phytopathol. Soc. Jpn. 65: 604-611 https://doi.org/10.3186/jjphytopath.65.604
  7. Park, E-J., Lee, S-D., Chung, E-J., Lee, M.-H. and Um, H-Y. 2007. Microtom-A model plant system to study bacterial wilt by Ralstonia solanacearum. Plant Pathol. J. 23: 239-244 https://doi.org/10.5423/PPJ.2007.23.4.239
  8. 서상태, 박종환, 한경숙, 정승룡, 이승돈. 2007. 우리나라에 분포하는 고추와 토마토 풋마름병균(Ralstonia solanacearum) 계통들의 유전적 다양성. 식물병연구 13: 24-29 https://doi.org/10.5423/RPD.2007.13.1.024
  9. Schonfeld, J., Heuer, H., Van Elsas, J. D. and Smalla, K. 2003. Specific and sensitive detection of Ralstonia solanacearum in soil on the basis of PCR amplification of fliC fragments. Appl.Envrion. Mcirobiol. 69: 7248-7256 https://doi.org/10.1128/AEM.69.12.7248-7256.2003
  10. Tans-Kersten, J., Huayu, H. and Caitilyn, A. 2001. Ralstonia solanacearum needs motility for invasive virulence on tomato. J. Bacteriol. 183: 3597-3605 https://doi.org/10.1128/JB.183.12.3597-3605.2001
  11. Villa, J., Tsuchiya, K., Hortia, M., Opina, N. and Hyakumachi, M. 2003. DNA analysis of Ralstonia solanacearum and related bacteria based on 282-bp PCR-amplified fragment. Plant Dis. 87: 1377-1343

Cited by

  1. Evaluation of Resistance to Ralstonia solanacearum in Tomato Genetic Resources at Seedling Stage vol.32, pp.1, 2016, https://doi.org/10.5423/PPJ.NT.06.2015.0121
  2. Physiological, Biochemical and Genetic Characteristics of Ralstonia solanacearum Strains Isolated from Pepper Plants in Korea vol.19, pp.4, 2013, https://doi.org/10.5423/RPD.2013.19.4.265