KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
권호 표지

Antibacterial, antioxidant and antitumor activities of mushroom mycelium mixed culture extracts

버섯 균사체 혼합 배양 추출물의 항균, 항산화 및 항암 활성

  • Kim, Man-Cheol (Faculty of marine science and Marine and environment institute, Cheju National University) ;
  • Kim, Ju-Sang (Faculty of marine science and Marine and environment institute, Cheju National University) ;
  • Heo, Moon-Soo (Faculty of marine science and Marine and environment institute, Cheju National University)
  • 김만철 (제주대학교 해양과학대학 해양과학부) ;
  • 김주상 (제주대학교 해양과학대학 해양과학부) ;
  • 허문수 (제주대학교 해양과학대학 해양과 환경연구소)
  • Published : 2008.04.29
Download PDF
⟨ Previous Next ⟩

Abstract

In this study, we have investigated the antibacterial, antioxidant, and antitumor activities of mycelium cultural extract from mushroom. Mushroom mycelium was grown in a synthetic liquid media such as PD broth, YM broth or citrus extracts. In antibacterial activity test, the best result was achieved when mycelium cultural extracts from Phellinus linteus and Coriolus versicolor were incubated together on YM broth. On the other hand, mushroom mycelium cultured on citrus extracts showed better activity than that on PD broth. We have also tested the antioxidant activity at concentration up to 10 mg of mycelium cultural extract/mL. The more it is in higher concentration, the more the activity increases. The higher antioxidant activity was observed both on PD broth containing the Phellinus linteus and Coriolus versicolor mycelium and citrus extract containing the same. The complex culture extracts obtained from the synthetic medium and citrus extract medium showed 10-89% of the 1,1-diphenyl-2-picrylhydrazyl radical scavenger activity. The antitumor activity of mycelium cultural extract was examined by using MTT assay on A549 cells. Mushroom mycelium cultured on citrus extracts showed interestingly higher antitumor activity than that on synthetic liquid media.

버섯 균사체 혼합배양액을 이용하여 어류질병세균에 대한 항균 및, 항산화활성, 그리고 인체 암세포에 대한 항암 활성을 조사하였다. 항균활성 측정 결과 대부분의 시료에서 항균활성이 있는 것으로 나타났다. 그 중 YM broth에서 배양한 상황버섯과 운지버섯 균사체 배양 추출물이 가장 높은 활성을 띄었지만 10% 감귤추출물배지에서 배양한 상황버섯과 운지버섯 균사체 배양 추출물 또한 비교적 활성이 높아 합성배지가 고가임을 고려할 때 의의가 있다고 생각된다. 항산화 활성의 측정결과 10% 감귤추출물배지에서 배양한 상황버섯, 운지버섯, 꽃송이 버섯 균사체 배양추출물과 PD broth에서 배양한 상황버섯과 운지버섯 균사체 배양 추출물의 경우 1 mg/ml에서 90%에 가까운 라디컬 소거활성을 보였다. 항암활성 측정결과 10% 감귤추출액만을 첨가한 대조군보다 모든 실험구가 높은 활성을 나타냈으며 특히 10% 감귤추출물배지에서 배양한 상황버섯, 운지버섯 배양 추출액과 10% 감귤추출물배지에서 배양한 상황버섯, 운지버섯, 꽃송이버섯 균사체 배양 추출액이 높은 활성을 나타났다. 이러한 다양한 생리활성을 측정한 결과 최종적으로 감귤농축액이 첨가된 버섯균사체 배양 추출물들의 활성이 가장 좋은 것으로 나타났으며, 감귤의 천연배지로서의 이용가능성을 확인할 수 있었다. 따라서 감귤농축액 및 버섯균사체 혼합 배양을 이용한 다양한 사료첨가제 및 신소재의 개발이 가능할 것이다.

Keywords

References

  1. Yu, H. E., S. M. Cho, G. S. Seo, B. S. Lee, D. H. Lee, and J. S. Lee (2006), Screening of bioactive compounds from mushroom Pholiota sp. Kor. J. Mycol. 34(1), 15-21 https://doi.org/10.4489/KJM.2006.34.1.015
  2. Lee, T. S. (1990), The full list of recoded mushroom in Korea, Kor. J. Mycol. 18, 233-259
  3. Ahn, D. K. (1992), Medicinal Fungi in Korea, Kor. J. Mycol. 20, 154-166
  4. Lucas, E. H., Ringle, R. U., Clarke, D. A., Reilly, H. C., Stevens, J. A., and Stock, C. C. (1957), Tumor inhibitors in Boletus edulis and other Holobasidiomycetes, Antibiot Chemotherapy 7, 1-4
  5. Chihara, G., Hamuro, J., Maeda, Y. Y., Arai, A., and Fukuoka, F. (1970), Fractionation and purification of the polysaccharides with made antitumor activity, especially lentinan, from Lentinus edodes (Berk) Sing (an edible mushroom), Res. Cancer 30, 2776-2781
  6. Fujii, T., Maeda, H., Suzuki, F., and Ishida, N. (1978), Isolation and characterization of a new antitumor polysaccharide, KS-2, Extracted from culture mycelia of Lentinus edodes L., Antibiotics 31, 1079-1085 https://doi.org/10.7164/antibiotics.31.1079
  7. Suzuki, S. and Oshima, S. (1976), Influence of Shitake (Lentinus edodes) on human serum cholesterol, Mushroom Sci. 9, 463-467
  8. Hikino, H., Kanno, C., Mirin, Y., and Hayashi, T. (1985), Isolation and hypoglycemic activity of Ganoderans A and B, glycans of Ganoderman lucidum fruit bodies, Planta. Med. 51, 339-340 https://doi.org/10.1055/s-2007-969507
  9. Lee, J. W., Chung, C. H., Jeong, H. J., and Lee, K. H. (1990), Anticomplementary and antitumor activities of the Ikal, extract from the mycelia of Lentinus edodes IY-105, Kor. J. Appl. Microbial. Biotechnol, 18, 571-577
  10. Kim, H. J., Ahn, M. S., Kim, G. H., and Kang, M. H. (2006), Physiological Activity/Nutrition : Antioxidative and Antimicrobial Activities of Pleurotus eryngii Extracts Prepared from Different Aerial Part Kor. J. Food Sci. Technol. 38(6), 799-804
  11. Song, C. H., Kim, J. H., Yang, B. K., and Kim, K. W. (1996), Anti-complementary polysaccharides produced from submerged mycelial culture of Pleurotus sajocaju. Korea J Mycology 24, 104-110
  12. Jung, I. C., Park, S., Park, K. S., and Ha, C. H. (1996), Antioxidative effect of fruit body and mycelia extracts of Pleurotus ostreatus. Korean J Food Sci Technol 28, 464-469
  13. Pyun, Y. R., Kwon, T. W., and Yu, J. H. (1977), Studies on a Mixed Yeast Culture; Part 1, Interactions in a Mixed Yeast Culture, Korea J. food sci. technol. 9(4), 306-312
  14. Koh, J. S. and S. H. Kim (1995), Physicochemical properties and chemical compositions of Citrus Fruits Produced in Cheju, Agric. Chem. Biotechnol. 38, 541-545
  15. Lee, S. J., S. H. Moon, T. Kim, J. Y. Kim, J. S. Seo, D. S. Kim, J. Kim, Y. J. Kim, and Y. I. Park (2003), Anticancer and antioxidant activities of Coriolus versicolor culture extracts cultivated in the citrus extract, Kor, J. Microbial. Biotechnol. 31(4), 362-367
  16. Song, E. Y., Y. H. Choi, K. H. Kang, and J. S. Koh (1998), Free sugar, organic acid, hesperidin, naringin and inorganic elements changes of Cheju Citrus Fruits according to harvest date, Kor. J. Food Sci. Technol. 30, 306-312
  17. Bios, M. S. (1958), Antioxidant determination by the use of a stable free radical, Nature 25, 1199-1120
  18. Chung, Y. C., C. T. Chang, W. W. Chao, and S. T. Chou (2002), Antioxidative activity and safety of the 50% ethanol extract from red bean fermented Bacillus subtilis IMR-NK 1, J. Agric. Food Chem. 50, 2454-2458 https://doi.org/10.1021/jf011369q
  19. Charmichael, J., Degeaff, W. G., Gazdar, A. F., Minna, J. D., and Michell, J. B. (1987), Evaluation of a tetrazolium-based semiautomated colorimetric assay, assessment of chemosensitivity testing, Cancer. Res. 47, 936-942
  20. Oh, H. S. and W. B. Park et al. (2003), Antimicrobial Activity of Extracts from Citrus Seeds, Korean J Culinary Research 9(4), 69-80
  21. Lee, J. H. and S. R. Lee (1994), Some Physiological Activity of Phenolic Substances in Plant Foods, Korean J. Food Sci. Technol. 26(3), 317-323
  22. Oh. I. S. and H. G. Kim (2006). Effect of Phellinus EXtracts on Sprouting in porcine pulmonary artery endothelial cells. Korean J. Biotechnology. Bioeng. 21(4), 292-297