Journal of the Korean Wood Science and Technology

The Korean Society of Wood Science & Technology (한국목재공학회)
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Antioxidant Activities of Essential Oils from Chamaecyparis obtusa

편백정유의 항산화활성

  • Park, Mi-Jin (Korea Forest Research Institute) ;
  • Choi, Won-Sil (Dept. of Forest Sciences, College of Agriculture & Life Sciences, Seoul National University) ;
  • Min, Byeong-Cheol (Dept. of Forest Sciences, College of Agriculture & Life Sciences, Seoul National University) ;
  • Kim, Ho-Yong (Dept. of Forest Sciences, College of Agriculture & Life Sciences, Seoul National University) ;
  • Kang, Ha-Young (Korea Forest Research Institute) ;
  • Choi, In-Gyu (Dept. of Forest Sciences, College of Agriculture & Life Sciences, Seoul National University)
  • 박미진 (국립산림과학원) ;
  • 최원실 (서울대학교 농업생명과학대학 산림과학부) ;
  • 민병철 (서울대학교 농업생명과학대학 산림과학부) ;
  • 김호용 (서울대학교 농업생명과학대학 산림과학부) ;
  • 강하영 (국립산림과학원) ;
  • 최인규 (서울대학교 농업생명과학대학 산림과학부)
  • Received : 2008.08.26
  • Accepted : 2008.10.06
  • Published : 2008.11.25
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Abstract

This study was carried out to investigate the potential promise of Chamaecyparis obtusa oil as a natural antioxidant. C. obtusa oil and its fractions were subjected to screening for their antioxidant activities by using 1,1-diphenyl-2-picrylhydrazyl (DPPH) method and ammonium thiocyanate method. In the first case, $IC_{50}$ value of the C. obtusa oil was determined as $40{\mu}{\ell}/m{\ell}$. At $0.5{\mu}{\ell}/m{\ell}$ concentration level, free radical scavenging effect of fraction G determined as 66.94% was the highest among the fractions of C. obtusa oil. In the ammonium thiocyanate method, essential oil of C. obtusa and its fraction C, D, and E showed activities of 72.0%, 71.2%, 71.9% and 71.1%, respectively. Fraction G, most active fraction, was mainly consisted of $\alpha$-terpineol, elemol, widdrol and $\alpha$-cadinol.

본 연구는 편백정유의 천연 항산화제로의 유용성을 평가하기 위하여 수행했다. 편백정유 원액과 편백정유 분획물의 항산화활성을 1,1-diphenyl-2-picrylhydrazyl (DPPH)법과 ammonium thiocyanate법을 사용하여 조사하였다. DPPH법을 이용한 경우 편백정유의 $IC_{50}$은 $40{\mu}{\ell}/m{\ell}$였으며, 편백정유 분획물 중에서 G분획의 DPPH 항산화능이 $0.5{\mu}{\ell}/m{\ell}$ 농도에서 66.94%로 가장 높았다. Ammonium thiocyanate법에서 배양 1일 후 편백정유 원액과 편백정유 분획물 C, D, E의 항산화효과는 각각 72.0%, 71.2%, 71.9%, 71.0%였다. 항산화 활성분획인 G분획은 $\alpha$-terpineol, elemol, widdrol, $\alpha$-cadinol 등으로 구성되어 있었다.

Keywords

Acknowledgement

Supported by : 농림부

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