Identification of Microorganisms from Eggs in Hypermarket in the Northern Gyeonggi Area

경기 북부 일부 지역 대형 마트 유통계란에 오염된 미생물의 분리

  • 전명숙 (한북대학교 식품영양학과) ;
  • 홍승희 (한북대학교 식품영양학과)
  • Published : 2009.09.30

Abstract

Microorganisms or their toxins can be transferred to eggs and cause food poisoning in humans. Therefore, this study was conducted to detect microbial contamination of eggs and to identify microorganisms in any contaminated eggs. Four different brands of eggs were collected from hypermarkets in the northern Gyeonggi area. The total bacterial counts on the shells of the eggs varied greatly between brands. In addition, various bacterial species including Klebsiella pneumoniae, Pseudomonas mendocina, Alcaligenes xylosoxidans, Alcaligenes faecalis, and Enterobacter cloacae were identified on eggshells. Furthermore, mean of total bacterial counts of four brands was $3.4{\times}10^4 cfu/m{\ell}$ and E. coli was detected on the eggshell of one brand egg. However, Salmonella was not identified on all brands of collected eggs. We also demonstrated that the E. coli isolated from the eggshell was not pathogenic based on the absence of pathogen-specific gene expression patterns. Taken together, the result of this study indicate that strict quality control and improved distribution controls are required to decrease microbial contamination and improve human health.

본 실험은 경기 북부 일부 지역의 계란에서 미생물 오염도를 측정하여 계란의 위생 상태를 파악하고자 하였다. 조사 자료로서는 대형 마트에 유통 중인 계란 중에서 서로 브랜드가 다른 계란을 대상으로 하였다. 계란의 난각에 존재하는 일반 세균수를 측정하였다. 그 결과 $3.4{\times}10^4cfu/m{\ell}$로 상당히 높은 일반 세균수가 검출되었다. 다음으로는 식중독 지수의 중요한 지표가 되는 대장균 및 계란으로 인한 식중독에서 주요 원인 균으로 작용하는 살모넬라균의 오염 여부를 파악한 결과, 한 브랜드의 계란에서 대장균이 검출되었고, 살모넬라균은 검출되지 않았다. 또한 각 브랜드에 계란에서 검출된 세균을 분리 동정 한 결과, Klebsiella pneumoniae, Pseudomonas mendocina, Alcaligenes xylosoxidans, Alcaligenes faecalis, Enterobacter cloacae 등이 분리되었다. 이 세균들 중 일부는 사람에게 병원성을 나타내거나, 면역력이 약한 노약자나 어린이에게 기회감염 또는 병원 내 감염을 일으키기도 하는 것으로 알려졌다. 계란의 난각에서 분리된 대장균의 병원성 여부를 판정하기 위하여, 대장균의 DNA를 분리하여 PCR을 수행한 결과, 병원성 대장균들에서 특이적으로 나타나는 유전자형을 가지고 있지 않는 것으로 밝혀졌다. 위의 결과들을 종합하여 보면, 시중에 유통중인 계란의 난각에서 비록 살모넬라균과 병원성 대장균은 검출되지 않았지만, 사람에게 감염을 일으킬 수 있는 여러 병원성 세균들이 검출됨으로써 계란의 유통 및 판매에 더욱 철저한 위생관리가 필요할 것으로 사료된다.

Keywords

References

  1. 김광협. 2000. 계란의 위생처리와 HACCP. 한국가금학회 춘계 산학협동 심포지움. pp.69-78
  2. 정민국. 2000. 유통환경 변화와 계란 유통구조의 개선방안. 한국가금학회 춘계 산학협동 심포지움. pp.53-66
  3. Arogone MR, Maurizi DM, Clara LO, Navarro Estrada JL, Ascione A. 1992. Pseudomonas mendocina, an environmental bacterium isolated from a patient with human infective endocarditis. J Clin Microbiol 30:1583-1584
  4. Bae JH. 2005. Antimicrobial effect of Plagiorhegama dubium extracts on food-borne pathogen. Korean J Food Nutr 18:81-87
  5. Baker RC, Goff JP. 1980. Prevalence of Salmonella on eggs from poultry farms in New York State. Poult Sci 59:289-292 https://doi.org/10.3382/ps.0590289
  6. Bang BH, Jeong EJ. 2009. Isolation and optimal producing conditions of broad spectrum antibiotics from Streptomyces sp. Y-88. Korean J Food Nutr 22:103-109
  7. Bean NH, Griffin PM. 1990. Foodborne disease outbreaks in United States, 1973-1987: pathogens, vehicles and trends. J Food Prot 53:804-817
  8. Cox NA, Baily JS, Thomson JE, Carson MO. 1980. Lactose preenrichment versus direct enrichment for recovering Salmonella from deep-chilled broilers and frozen meat products. Poult Sci 59:2431-2436 https://doi.org/10.3382/ps.0592431
  9. Du Reu K, Messens W, Heyndrickx M, Rodenburg TB, Uyttendaele M, Herman L. 2008. Bacterial contamination of table eggs and the influence of housing systems. World Poult Sci J 64:5-19 https://doi.org/10.1017/S0043933907001687
  10. Henzler DJ, Ebel E, Sanders J. 1994. Salmonella enteritidis in eggs farm commercial chicken layer flocks implicated in human outbreaks. Avian Dis 38:37-43 https://doi.org/10.2307/1591834
  11. Humphrey TJ. 1990. Public health implications of the infection of egg-laying hens with Salmonella enteritidis phage type 4. World Poult Sci J 46:5-13 https://doi.org/10.1079/WPS19900002
  12. Humphrey TJ, Martin KW, Whitehead A. 1994. Contamination of hands and work surfaces with Salmonella enteritidis PT4 during the preparation of egg dishes. Epidemol Infect 113:403-409 https://doi.org/10.1017/S0950268800068412
  13. Jang KI, Park JH, Kim KY. 1999. Studies on Salmonella enteritidis contamination in chicken egg using confocal scanning laser microscopy. Korean J Food Sci Technol 31:771-777
  14. Jones FT, Rives DV, Carey JB. 1995. Salmonella contamination in commercial eggs and an egg production facility. Poult Sci 74:753-757 https://doi.org/10.3382/ps.0740753
  15. Lammerding AM, Garcia MM, Mann ED, Robinson Y, Dorward WJ, Truscott RB, Tittiger F. 1988. Prevalence of Salmonella and thermophilic Campylobacter in fresh pork, beef, veal and poultry in Canada. J Food Prot 51:47-52
  16. Lee SM, Kim KH, Lee JG, Park EJ, Lee SW, Hong CH. 2002. Hygienic quality of eggs in the department food stores in the Incheon Metropolitan area. J Food Hyg Safety 17:129-136
  17. Papadopoulou C, Dimitriou D, Levidiotou S, Gessouli H, Panagiou A, Glegou S, Antoniades G. 1997. Bacterial strains isolated from eggs and their resistance to currently used antibiotics: Is there a health hazard for consumer? Comp Immunol Microbiol Infect Dis 20:35-40 https://doi.org/10.1016/S0147-9571(96)00024-0
  18. Saiman L, Chen Y, Tabibi S, Gabriel PS, Zhou J, Liu Z, Lai L, Whitter S. 2001. Identification and antimicrobial susceptibility of Alcaligenes xylosoxidans isolated from patients with cystic fibrosis. J Clin Microbiol 39:3942-3945 https://doi.org/10.1128/JCM.39.11.3942-3945.2001
  19. Tsay RW, Lin LC, Chiou CS, Liao JC, Chen CH, Liu CE, Young TG. 2005. Alcaligenes xylosoxidans bacteremia: clinical features and microbiological characteristics of isolates. J Microbiol Immunol Infect 38:194-199
  20. Woo IH, Hwang YK, Lee YS. 2008. The status and the actual sanitation management conditions of food services within the metropolitan area. Korean J Food Nutr 21:355-365
  21. Woo YK. 2005. Microbial hygienic status of poultry meats and eggs collected at the public markets in Seoul and Gyeong-gi regions in 1996. Kor J Microbiol 41:38-46