Kidney Research and Clinical Practice

Korean Society of Nephrology (대한신장학회)
권호 표지

The Effect of Extract of Paeonia lactiflora on the Improvement of Ischemic Acute Renal Failure

허혈성 급성신부전에 대한 작약추출물의 영향

Jung, Hyun-Joo;Kim, Min-Ji;Lim, Sun-Ha;Park, Jun-Hong;Lee, Hyeong-Kyu;Ahn, Ki-Sung;Lee, Jong-Won
정현주;김민지;임선하;박준홍;이형규;안기성;이종원

  • Published : 20090500
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Abstract

Purpose : The present study was undertaken to investigate whether the extract or the ethyl acetate fraction of Paeonia lactiflora, which improves cell survival under ischemic condition by inhibiting apoptosis, can prevent ischemic acute renal failure, using rats as an animal model. Methods : In the control group, ischemia/reperfusion (I/R) injury was induced in male Sprague-Dawley rats by clamping of left renal pedicle for 45 minutes after removal of the right kidney, and subsequent reperfusion of the pedicle for 24 hours. In the experimental group, the water extract or ethyl acetate fraction of methanol extract Paeonia lactiflora was injected 1 hour prior to ischemia/reperfusion injury. We measured serum concentrations of creatinine at 24 hours after I/R injury. And the kidneys were extracted, fixed in a 10 % neutral-buffered formalin solution, embedded in paraffin and used for histopathological examination. Results : Rats in the experimental group, treated with water extract or ethyl acetate fraction of methanol extract of Paeonia lactiflora, exhibited significant decrease in the serum concentrations of creatinine (approx. 2.0 mg/dL), compared with those in the control group (approx. 4.0 mg/dL). Finally, the cell morphology of the kidney of rats treated with ethyl acetate fraction of Paeonia lactiflora was well preserved, when judged from histopathological point of view. Conclusion : Pretreatment of rats with the water extract or ethyl acetate fraction of Paeonia lactiflora might be beneficial for the treatment of acute renal failure in humans.

목 적: 천연물 중 작약이 저산소 상태에서 세포 자살을 억제함으로써 세포의 생존을 개선시키는 것을 관찰하고 이 기전을 허혈성 급성신부전 모델에 적용하여 작약이 증상을 개선시키는지에 대해 조사하고자 하였다. 방 법: 백서에서 우측 신장을 적출한 후 좌측 신동맥을 겸 자하여 허혈/재관류 손상을 유발하였다. 허혈 1시간 전에 작약의 열수 조추출물을 투여하여 허혈/재관류 손상을 주고 24시간 뒤 혈액을 채취하여 혈중 크레아티닌치를 측정하였다. 이 때 음성대조군은 생리식염수를 투여하였다. 작약의 메탄올 추출물의 에틸아세테이트 분획물도 위와 동일한 방법으로 실험을 하고 H&E 염색을 통하여 조직의 변화를 관찰하였다. 결 과: 저산소 상태에서 세포 자살을 억제함으로써 세포의 생존을 개선시키는 작약의 열수 조추출물을 허혈성 급성신부전을 일으킨 백서의 모델에 투여하였을 때 음성대조군은 혈중 크레아티닌치가 3.9${\pm}$0.5 mg/dL로 나왔으나 작약의 열수조추출물을 투여한 군은 그 수치가 1.9${\pm}$0.9 mg/dL로 떨어지게 나타났다. 작약의 메탄올 추출물 중 에틸아세테이트 분획물도 음성 대조군에 비해 혈중 크레아티닌치를 51% 정도 줄이는 효과를 나타내었다. 또한 조직학적인 측면에서도 음성 대조군에 비해 외수질 지역에서 핵의 유실 및 괴사가 진행된 곳이 적었다. 결 론: 천연물 중 세포 자살을 억제하는 작약 조추출물 및 그 분획물이 허혈성 급성신부전에서 혈중 크레아티닌치 및 조직을 개선시키는 것을 확인하였다. 이는 뚜렷한 치료제가 개발되지 않은 급성신부전의 예방 및 치료제에 새로운 이점을 줄 것으로 기대되고 이를 위해서 독성 및 그 기전에 대한 더 많은 연구도 필요할 것으로 사료된다.

Keywords

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