Analysis of Agricultural Characteristics for the Virus Resistant GM Pepper Plants Grown in Three Different Regions in Korea

바이러스 내병성 GM 고추의 중부와 북부 지역에서의 농업적 재배특성조사 분석

Cho, Dong-Wook;Oh, Jin-Pyo;Park, Kuen-Woo;Chung, Kyu-Hwan
조동욱;오진표;박권우;정규환

  • Published : 2009.12.31

Abstract

This study was to investigate agricultural characteristics between 2 lines of cucumber mosaic virus (CMV) resistant transgenic hot pepper plants and their control pepper plants grown in three different regions of Korea in order to establish the evaluating protocol and standard assessment for the genetically modified (GM) hot pepper. In 2007 and 2008, agricultural characteristics of 2 GM pepper plants carrying CMV coat protein (CP) gene and their control plants were investigated. The collected data was analyzed to compare each characteristic between GM and non-GM pepper plants in the same field and in three different regions. From the statistic analysis, there were not many significant differences between GM and non-GM peppers. However, there were few differences found in some of the quality investigation items. These exceptions included investigation items of vegetative growth period and fruit setting period of red peppers. On the other hand, there were apparent differences shown among all three regions in 2007 and 2008. Upon comparing the characteristics of GM and non-GM peppers collected from three different regions, the value of each characteristic shown in GM and non-GM peppers from the northern region was higher than the southern region. This was due to the low temperature in the northern region compared to the southern region. In yield of GM and non-GM peppers in each region, total fruit weight of GM pepper was higher than non-GM pepper. Moreover, the total fruit weight of green and red peppers from 2377 line (H-15 and P-2377) were higher than 915 line. Also the total fruit weight of GM and non-GM peppers from three different regions in 2007 and 2008 varied. In conclusion, there were not significant differences between GM and non-GM peppers in terms of agricultural characteristics.

본 연구는 CMV-CP 유전자가 형질전환된 CMV 내병성 고추 2계통과 각 계통의 모본에 대한 농업적 특성을 2년 동안 중부지역 2곳과 북부지역 1곳에서 재배하여 조사, 비교함으로써 GM 작물의 평가 프로토콜 확립을 위한 기초 자료로 이용하고자 실시하였다. 2007년과 2008년 GM 고추 2계통과 그 모본에 대한 자료를 조사 분석하여 동일지역 내에서의 GM과 non-GM간의 차이점을 비교하였으며, 또한 2007년과 2008년도 및 지역 간의 차이점을 비교 분석하였다. 조사결과 각 지역에서는 GM과 non-GM간의 여러 항목에서 유의차를 보였지만 전반적으로는 큰 차이는 보이지 않았다. 하지만 지역 간의 유의차는 여러 항목에서 다소 크게 나타났다. 특히 식물체 생장특성과 숙과의 특성분석에서는 GM과 non-GM 간의 여러 항목에서 같은 지역 내에서 약간의 유의차를 보였다. 하지만 잎의 특성, 미숙과의 특성 및 종자의 특성분석은 GM과 non-GM간에 몇몇 항목에서 미미한 차이를 보였을 뿐 전반적으로 유의차를 보이지 않았다. 특히 지역 간의 비교분석에서 북부지역이 중부지역에 비하여 많은 항목에서 높은 값을 나타냈다. 이러한 차이점은 지역 간의 연평균 온도차에 의한 변이라 생각된다. 각 지역 내에서 고추의 총 수확량을 비교한 결과 GM 고추의 총 수확량이 non-GM 고추보다 월등히 많았다. 특히 915 계통에 비하여 2377 계통의 수확량이 더 많았다. 지역 간의 변이 역시 크게 나타났다. 결론적으로 GM 작물의 평가프로토콜 작성을 위하여 중부 및 북부 지역 내에서의 GM 고추와 non-GM 고추간의 농업적 특성을 조사, 분석한 결과 큰 차이점은 나타나지 않았다.

Keywords

References

  1. Abdalla, O.A., P.R. Desjardine, and J.A. Dodds. 1991. Identification, disease incidence, and distribution of viruses infecting peppers in California. Plant Dis. 75:1019-1023 https://doi.org/10.1094/PD-75-1019
  2. Alonso, E., L.I. Gracia, R.M.J. Avila, B. Wicke, M.T. Serra, and Kor. J. Hort. Sci. Technol. 27(4), December 2009 529 R.J.R. Diaz. 1989. A tobamovirus causing heavy losses in protected pepper crops in Spain. Journal of Phytopathology 125:67-76 https://doi.org/10.1111/j.1439-0434.1989.tb01057.x
  3. Benner, C.P., C.W. Kuhn, J.W. Demski, J.W. Dobson, P. Colditz, and F.W Nutter, Jr. 1985. Identification and incidence of pepper viruses in northeastern Georgia. Plant Dis. 69:999-1001
  4. Boccardo, G. and M. Conti. 1973. Purification and properties of nasturtium ringpot virus. Phytopathology Z. 78:14-24 https://doi.org/10.1111/j.1439-0434.1973.tb04146.x
  5. Cai, W.Q., R.X. Fang, H.S. Shang, X. Wang, F.L. Zhang, Y.R. Li, J.C. Zhang, X.Y. Cheng, G.L. Wang, and K.Q, Mang. 2003. Development of CMV- and TMV-resistant transgenic chili pepper: Field performance and biosafety assessment. Mol.Breed. 11:25-35 https://doi.org/10.1023/A:1022655204552
  6. Chen, Z.L., H. Gu, Y. Li, Y. Su, P. Wu, Z. Jiang, X. Ming, J. Tian, N. Pan, and L.J. Qu. 2003. Safety assessment for genetically modified sweet pepper and tomato. Toxicology 188:297-307 https://doi.org/10.1016/S0300-483X(03)00111-2
  7. Cho J.D., S.H Lee, J.S. Kim, K.S. Choi, and B.N. Chung. 2007. Study of occurrence of virus disease on the pepper in Korea, http://bric.postech.ac.kr/webzine/, BioWave 9(11):1-8
  8. Choi, G.S., J.H. Kim, D.H. Lee, J.S. Kim, and K.H. Ryu. 2005. Occurrence and distribution of viruses infecting pepper in Korea. Plant Pathol. J. 21(3):258-261 https://doi.org/10.5423/PPJ.2005.21.3.258
  9. Choi, J.K., M.Y. Sung, H.J. Jung, J.S. Hong, and S.Y. Lee. 2001. Cross-protection effectiveness Cucumber mosaic virus (CMV) isolates associated with Satellinte RNA for prevention of CMV disease in pepper plants. Res. Plant Dis. 7(3):155-163
  10. Florini, D.A. and T.A. Zitter. 1987. Cucumber mosaic virus (CMV) in pepper (Capsicum annuum L.) in New York and associated yield losses. Phytopathoogy 77:652 (Abstr.)
  11. Gracia, O. and L.S. Gutierrez. 1982. Broad bean wilt virus in pepper crops in Argentina. Phytopath. Medit. 21:107-109
  12. Hwang, J.M. and B.Y. Lee. 1978. Studies on some horticultural characters influencing quality and yield in the pepper (Capsicum annuum L.). II. Correlations and selection. J. Kor. Soc. Hort. Sci. 19:48-55
  13. Imoto, M. 1975. Studies on mosaic disease of sweet pepper (Capsicum frutescens L.) 2. Occurrence of mosaic disease of sweet pepper and kinds of its causal viruses in Hiroshima prefecture. Bull. Hiroshima Agr. Expt. Station, Japan. 36:56-66
  14. Jang, K.S., D.J. Choi, D.H. Pae, J.T. Yoon, and S.K. Lee. 2000. Effects of altitudes on growth and fruit quality in red pepper (Capsicum annuum L.). J. Kor. Soc. Hort. Sci. 41(5):485-489
  15. Joshi, R.D. and L.N. Dubey. 1973. Assessment of losses due to CMV on chili. Science and Culture 39:521-522
  16. Kim D.H., J.D. Cho, J.H. Kim, J.S. Kim, and E.K. Cho. 2005. Ultrastructural characteristics of necrosis and stunt disease in red pepper by the mixed infections of Tobacco mosaic virus-U1 and Pepper mild mottle virus and Pepper mottle virus. Plant Pathol. J. 21(3):252-257 https://doi.org/10.5423/PPJ.2005.21.3.252
  17. Kim, H.S., D.H. Pae, D.J. Choi, K.S. Jang, J.L. Cho, and T. Kim. 1999. Growth, yield and quality of tunnel-cultured red pepper (Capsicum annuum L.) as affected by plant spatial arrangement. J. Kor. Soc. Hort. Sci. 40(6):657-661
  18. Laird, E.F., P.R. Desjardins, and R.C. Dickson. 1964. Tobacco etch virus and potato virus Y from pepper in southern California. Plant Dis. Rep. 48:772-776
  19. Lee S.H., J.B. Lee, S.M. Kim, H.S Choi, J.W. Park, J.S. Lee, K.W. Lee, and J.S. Moon. 2004. The incidence and distribution of viral diseases in pepper by cultivation types, Res. Plant Dis. 10(4):231-240 https://doi.org/10.5423/RPD.2004.10.4.231
  20. Makkouk, K.M. and D.J. Gumpf. 1976. Characterization of potato virus Y strains isolated from pepper. Phytopathology 66:576-581 https://doi.org/10.1094/Phyto-66-576
  21. Palukaitis, P., M.J. Roossinck, R.G. Dietzgen, and R.I.B. Francki. 1992. Cucumovirus. Adv. Virus Res. 41:281-348 https://doi.org/10.1016/S0065-3527(08)60039-1
  22. Pares, R.D. 1985. A tobamovirus infecting capsicum in Australia. Ann. Appl. Biol. 106:459-474
  23. Purcifull, D.E., T.A. Zitter, and E. Hiebert. 1975. Morphology, host range and serological relationships of pepper mottle virus. Phytopathology 65:559-562 https://doi.org/10.1094/Phyto-65-559
  24. Schuerger, A.C. and W. Hammer. 1995. Effects of temperature on disease development of tomato mosaic virus in Capsicum annuum in hydroponic systems. Plant Dis. 79(9):880-885 https://doi.org/10.1094/PD-79-0880
  25. Yankulova, M. and P. Kaitazova. 1979. Broad bean wilt virus-a new virus on pepper in Bulgaria. Grradinarska I Lozarska Nanka. 16:48-57