Journal of The Korean Ophthalmological Society (대한안과학회지)

The Korean Ophthalmological Society (대한안과학회)
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Effect of Glutathione With Sea Tangle Extract on Prevention of Selenite-Induced Cataract Formation in Rats

쥐의 산화스트레스성 백내장 발생 모델에서 다시마 추출물을 이용한 글루타티온의 백내장 예방효과

Kim, Ei-Tae;Oh, Jung-Hyub
김이태;오중협

  • Published : 2009.10.15
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Abstract

Purpose: To evaluate antioxidative and preventive effects of sea tangle extract on selenite-induced cataract formation. Methods: Eighty SD rat pups were randomized into 8 groups. Group 1 received no injection of reagent (normal); Group 2 to 8 received injection of selenite (15 ${\mu}mol$/Kg, s.c.) was injected. In group 2 (control) and group 3, normal saline (i.p.) and ascorbic acid (i.p.) was injected on days 3$\sim$31. In groups 4$\sim$8, sea tangle extract (i.p.) was injected at a concentration of 12.5, 25, 50, 100, 200 mg/kg, respectively. Development of cataract was assessed and photographed weekly under slit lamp. Rat lenses were analyzed for antioxidant enzymes, glutathione peroxidase (GPx), superoxide dismutase and malondialdehyde. Furthermore, an amino acid analysis of sea tangle extract was performed. Results: Significant differences (p <0.05) were seen in cataract development in group 7. Dense nuclear cataracts developed in 8 of 10 of the control group (group 2); Group 4$\sim$8 developed nuclear cataract with proportion of 6/10, 3/10, 2/10, 1/10, and 6/10 rats. In sea tangle injected group, levels of GPx were higher than in the ascorbic acid and control groups. In particular, group 7, injected with 100 mg/kg of sea tangle extract, showed significantly high level of enzyme. Results of the amino acid analysis showed sea tangle includes glutamate-glycine-cysteine, major constituents of glutathione (GSH). Conclusions: The glutamate-glycine-cysteine in sea tangle is supposed to increase the level of lens GSH and this may contribute to lowering cataract development. This study strongly supports the activity of sea tangle as an endogenous antioxidant and anticataract agent.

목적: 다시마 추출물을 이용하여 산화 스트레스로 인한 백내장의 예방효과에 대해 알아보고자 하였다. 대상과 방법: 대조군과 실험군의 8군으로 백서 10마리씩 무작위로 분류하여 대조군에는 셀레나이트 주사 후 생리식염수만 투여하였고실험군 4$\sim$8군은 각각 다시마 추출물 12.5, 25, 50, 100, 200 mg/Kg의 농도로 복강내 주사하였다. 세극등현미경검사를 시행하여 백내장 발생 정도를 비교하였고, 수정체를 채취하여 항산화효소와 말론디알데히드의 농도를 비교하였다. 또한 다시마 추출물의 아미노산 성분 분석을 실시하였다. 결과: 100 mg/Kg 농도의 다시마 추출물을 투여한 실험군 7에서 백내장이 적게 발생하였고 항산화 효소의 활성도가 높게 나타났다. 다시마 추출물의 농도가 높아짐에 따라 백내장 발생이 감소하는 양상을 보였으나 추출물의 농도가 너무 높거나 낮으면 오히려 백내장발생이 증가하였다. 다시마 추출물의 아미노산 성분 분석 결과 글루타티온의 구성물질인 글루타메이트, 글라이신, 시스테인이 포함되어 있었다. 결론: 다시마 추출물은 글루타티온의 활성도를 높임으로써 항산화 효과를 보이며 산화 스트레스성 백내장을 효과적으로 예방할 수 있었다.

Keywords

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