Annals of Laboratory Medicine

Korean Society for Laboratory Medicine (대한진단검사의학회)
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Allergen Specific IgE Measurement with Polycheck Allergy: Comparison of Three Multiple Allergen Simultaneous Tests

Polycheck Allergy를 이용한 알레르겐 특이 IgE 측정: 세 가지 다중 알레르겐 항원 검사법의 비교

Jang, Woo-Ri;Nahm, Chung-Hyun;Kim, Jung-Hee;Lim, Dae-Hyun;Jang, Tae-Young;Moon, Yeon-Sook;Kim, Jin-Ju
장우리;남정현;김정희;임대현;장태영;문연숙;김진주

  • Published : 20091000
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Abstract

Background : The in vivo skin prick test (SPT) or in vitro detection of allergen specific IgE in serum is commonly used for the diagnosis of allergic disease. In this study, we evaluated the usefulness of a new multiple allergen simultaneous test (MAST) immunoblot assay, Polycheck Allergy (Biocheck GmbH, Germany). Methods : A total of 100 patients with clinical findings of allergic diseases were tested by SPT and three different MAST assays: Polycheck Allergy (Biocheck GmbH, Germany), MAST CLA allergy system (Hitachi Chemical Diagnostics, USA) and Allergy Screen (R-biopharm, Germany). The results of MAST assays were compared with those of SPT. Results : Concordance rates of MAST assays with SPT were 79-100% for Polycheck Allergy, 88.9-100% for MAST CLA and 72.7-98.3% for Allergy Screen. In ROC curve analysis, significant differences were observed in four of 25 allergens analysed: Alternaria, Birch, Hazelnut and D. farinae. For Alternaria and Birch, Polycheck Allergy (P<0.001) and Allergy Screen (P=0.0075) showed significantly larger AUC (area under the curve) than MAST CLA. For Hazelnut, Polycheck Allergy (P=0.0021), and for D. farinae, MAST CLA (P=0.015) showed significantly larger AUCs than the other two tests. The ROC analysis for overall 16 food allergens showed better results in Polycheck Allergy (P<0.001), and that for overall 21 inhalants did not show significant differences among three MAST assays (P>0.05). Conclusions : Since Polycheck Allergy showed similar or superior result to the others, it can be used for the detection of allergen specific IgE antibodies.

배경 : 피부단자시험 또는 혈청 알레르겐 특이 IgE를 확인하는 검사가 알레르기 질환의 진단에 흔히 사용된다. 저자들은 최근 국내에 소개된 다중 알레르기항원 검사 immunoblot 검사법의 일종인 Polycheck Allergy (Biocheck GmbH, Mu_nster, Germany)의 유용성에 대해 알아보고자 하였다. 방법 : 임상적으로 알레르기 증상을 보이는 100명의 환자를 대상으로 피부단자시험과 Polycheck Allergy (Biocheck GmbH, Germany), MAST CLA allergy system (Hitachi Chemical Diagnostics, Inc., USA), Allergy Screen (R-biopharm, Germany) 을 시행한 후 검사 결과를 피부단자시험 결과와 비교하였다. 결과 : Polycheck Allergy, MAST CLA 그리고 Allergy Screen의 피부단자시험과의 일치율은 각각 79-100%, 88.9-100%, 72.7-98.3%의 분포를 보였다. 각각의 알레르겐에 대한 ROC 분석 결과 네 가지에서만 유의한 차이를 보였다. Alternaria와Birch에서는 Polycheck Allergy와 Allergy Screen의 곡선하 면적이 MAST CLA보다 유의하게 컸고(각각 P<0.001, P=0.0075) Hazelnut은 Polycheck Allergy에서(P=0.0021), 그리고 D. farinae는 MAST CLA에서(P=0.015) 곡선하 면적이 다른 두 시약보다 컸다. 음식형 알레르겐 16종의 통합적인 ROC 분석에서는 Polycheck Allergy가 가장 좋은 성적을 보였고(P<0.001), 21종의 흡입형 알레르겐에서는 세 검사 간에 유의한 차이가 없었다(P>0.05). 결론 : Polycheck Allergy는 기존의 MAST CLA 및 Allergy Screen과 비슷하거나 우수한 성적을 보여 혈청 알레르겐 특이 IgE 검출에 효율적으로 사용할 수 있을 것으로 기대되었다.

Keywords

References

  1. Kim HS, Park CW, Lee JS, Cho YJ. The sensitization rate to house dust mites and the prevalence of asthma in healthy young women. Korean J Asthma Allergy Clin Immunol 2004;24:103-9. (김희선, 박창환, 이지수, 조영주. 알레르기 병력이 없는 젊은 여성에서의 집먼지 진드기감작률및천식유병률. 천식및알레르기 2004;24:103-9.)
  2. Lee SI, Shin MH, Lee HB, Lee JS, Son BK, Koh YY, et al. Prevalences of symptoms of asthma and other allergic diseases in korean children:a nationwide questionnaire survey. J Korean Med Sci 2001;16:155-64 https://doi.org/10.3346/jkms.2001.16.2.155
  3. Brown CR, Higgins KW, Frazer K, Schoelz LK, Dyminski JW, Marinkovich VA, et al. Simultaneous determination of total IgE and allergen-specific IgE in serum by the MAST chemiluminescent assay system. Clin Chem 1985;31:1500-5
  4. Lim HS, Kim HS, Oh H. Current status of serum allergen tests in Korea. Korean J Lab Med 2008;28:124-9. (임환섭, 김현수, 오흥범. 국내 혈청 알레르기 항원 검사법의 현황. 대한진단검사의학회지 2008;28:124-9.) https://doi.org/10.3343/kjlm.2008.28.2.124
  5. Ramesh S. Food allergy overview in children. Clin Rev Allergy Immunol 2008;34:217-30 https://doi.org/10.1007/s12016-007-8034-1
  6. Min YG, Choi BY, Kwon SK, Lee SS, Jung YH, Kim JW, et al. Multicenter study on the prevalence of perennial allergic rhinitis and allergy-associated disorders. J Korean Med Sci 2001;16:697-701 https://doi.org/10.3346/jkms.2001.16.6.697
  7. Ownby DR. Allergy testing: in vivo versus in vitro. Pediatr Clin North Am 1988;35:995-1009 https://doi.org/10.1016/S0031-3955(16)36544-0
  8. Garcia-Marcos L, Sanchez-Solis M, Martinez-Torres AE, Lucas Moreno JM, Hernando Sastre V. Phadiatop compared to skin-prick test as a tool for diagnosing atopy in epidemiological studies in schoolchildren. Pediatr Allergy Immunol 2007;18:240-4 https://doi.org/10.1111/j.1399-3038.2006.00508.x
  9. Reddy PM, Nagaya H, Pascual HC, Lee SK, Gupta S, Lauridsen JI, et al. Reappraisal of intracutaneous tests in the diagnosis of reaginic allergy. J Allergy Clin Immunol 1978;61:36-41 https://doi.org/10.1016/0091-6749(78)90471-2
  10. Contin-Bordes C, Petersen A, Chahine I, Boralevi F, Chahine H, Taieb A, et al. Comparison of ADVIA Centaur and Pharmacia UniCAP tests in the diagnosis of food allergy in children with atopic dermatitis. Pediatr Allergy Immunol 2007;18:614-20 https://doi.org/10.1111/j.1399-3038.2007.00552.x
  11. Dreborg S. Histamine reactivity of the skin. Allergy 2001;56:359-64 https://doi.org/10.1034/j.1398-9995.2001.056005359.x
  12. Park DS, Cho JH, Lee KE, Ko OS, Kim HR, Choi SI, et al. Detection rate of allergen-specific IgE by multiple antigen siumltaneous testimmunoblot assay. Korean J Lab Med 2004;24:131-8. (박도심, 조지현, 이기은, 고옥순, 김학렬, 최삼임 등. Multiple Antigen Simultaneous Test-Immunoblot법을 이용한 알러젠 특이항체 검출. 대한진단검사의학회지 2004;24:131-8.)
  13. Kim HS, Kim DJ, Lee SG. Analysis of simultaneous positivity to multiple allergens on MAST CLA test. Korean J Lab Med 2005;25:448-56. (김현수, 김대중, 이성규. MAST CLA 알레르기 검사에서 동시양성알레르겐의분석. 대한진단검사의학회지 2005;25:448-56.)
  14. Sampson HA. Food allergy--accurately identifying clinical reactivity. Allergy 2005;60(S):S19-24 https://doi.org/10.1111/j.1398-9995.2005.00853.x
  15. Lee SR, Lee HR, Keum DG. Evaluation of the MAST-CLA allergy system (Korea IgE panel) for diagnosis of atopic allergy. Korean J Clin Pathol 1995;15:469-77. (이소라, 이혜림, 금동극. 아토피성 알레르기 진단에 있어서 MAST-CLA 알레르기 검사(한국형)에 대한 평가. 대한임상병리학회지 1995;15:469-77.)
  16. Benjamini Y and Hochberg Y. Controlling the false discovery rate:a practical and powerful approach to multiple testing. J R Statist Soc B 1995;57:289-300

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