Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
권호 표지

Antioxidant and Antidiabetic Effects of Various Sections of Astragalus membranaceus

연근별 황기의 항산화 및 항당뇨 활성 평가 고찰

  • Yin, Yu (School of Biotechnology, Kangwon National University) ;
  • Heo, Seong-Il (School of Biotechnology, Kangwon National University) ;
  • Jung, Mee-Jung (School of Biotechnology, Kangwon National University) ;
  • Wang, Myeong-Hyeon (School of Biotechnology, Kangwon National University)
  • 윤유 (강원대학교 생명공학부) ;
  • 허성일 (강원대학교 생명공학부) ;
  • 정미정 (강원대학교 생명공학부) ;
  • 왕명현 (강원대학교 생명공학부)
  • Published : 2009.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

The antioxidant and anti-diabetes effects were evaluated by the ethanol extracts from Astragalus membranaceus root classified by years using through DPPH radical scavenging activity, hydroxyl radical (${\cdot}OH$) scavenging activity, total phenolic and flavonoid contents, reducing power activity, $\alpha$-amylase and $\alpha$-glucosidase inhibitory activities. $IC_{50}$ values for DPPH radical scavenging activity of ethanol extract from 6 years old root ($749.25{\mu}g/mL$) was higher than 1 year ($1452.67{\mu}g/mL$) and 3 years old root ($1095.61{\mu}g/mL$). 6 years old root showed better effects in ${\cdot}OH$ scavenging activity ($IC_{50}$: $10.58{\mu}g/mL$), reducing power, total phenolic contents ($26.13{\pm}0.79\;Tan\;{\mu}g/mg$, $24.03{\pm}0.74\;Cat\;{\mu}g/mg$) $\alpha$-amylase ($33.33{\pm}0.55%$) and $\alpha$-glucosidase inhibitory activity ($49.71{\pm}1.01%$). On the other hand, total flavonoid compound contents were estimated much higher in 1 year old root ($44.93{\pm}1.35\;Que\;{\mu}g/mg$, $70.32{\pm}2.03\;Rut\;{\mu}g/mg$) than others. Based on these results, It was suggested that 6 years old root of A. membranaceus has a potential candidate for functional cosmetic and medicine.

Keywords

References

  1. Evance, C. R., Halliwell, B. and Lunt, G. G. (1995) Free radical and oxidative stress: environment, drugs and food additives. Portland Press, London, England
  2. Sozmen, E. Y., Tanyakin, T., Onat, T., Kufay, F. and Frlacin, S. (1994) Ethanoli-induced oxidative stress and membrane injury in rat erythrocytes. Eur. J. Clin. Chem. Clin. Biochem. 32: 741-744 https://doi.org/10.1515/cclm.1994.32.10.741
  3. Kim, H. K., Kwon, Y. J., Kim, Y. E. and Nahmang, B. (2004) Changes of total polyphenol content and antioxidant activity of Aster scaber Thumb extracts with different microwave assisted extraction conditions. Korea J. Food Preserv. 11: 88-93
  4. Kim. T. K., Shin, H. D. and Lee, Y. H. (2003) Stabilization of polyphenolic antioxidants using inclusion complexation with cyclodextrin and their utilization as the fresh-food preservation. Korean J. Food Sci. Technol. 35: 266-271
  5. Cha, B. C. and Lee, E. H. (2007) Antioxidant activities of flavonoids from the leaves of Smilax china Linne. Kor. J. Pharmacogn. 38: 31-36
  6. Lee, Y. M., Shin, H. D., Lee, J. J. and Lee, M. Y. (2007) Antioxidative effect of Chaenomelis fructus ethanol extract. Korea J. Food Preserv. 14: 177-182
  7. Park, Y., Boo, H. O., Park, Y. L., Cho, D. H. and Lee, H. H. (2007) Antioxidant activity of Momordica charantia L. extracts. Korean J. Medicinal Crop Sci. 15: 56-61
  8. Yang, H. J. and Park, S. N. (2007) Evaluation of antioxidant potential of extract/fractions of Equisetum arvense (I). Korea J. Soc. Cosmet. Chem. 33: 61-67
  9. Seo, H. S., Chung, B. H. and Cho, Y. G. (2008) Antioxidant and anticancer effects of Agrimony (Agrimonia pilosa L.) and Chinese lizardtail (Saururus chinenesis Baill). Korean J. Medicinal Crop Sci. 16: 139-143
  10. Lim, A. K., Kim, J. O., Jung, M. J., Jung, H. K., Hong, J. H. and Kim, D. I. (2008) Functional biological activity of hot water and ethanol extracts from Taraxaci herba. J. Food Sci. Nutr. 37: 1231-1237
  11. Kim, J. S. and Kim, C. S. (1997) A study on the constituents from the roots of Astragalus membranaceus (II). Kor. J. Pharmacogn. 28: 75-79
  12. Min, S. H. and Lee, B. R. (2008) Effect of Astragalus membranaceus powder on yeast bread baking quality. Korean J. Food Culture 23: 228-234
  13. 대한한의과 대학 교재편찬위원회 (2005) 본초학. 연림사. 서울
  14. Cui, B., Inoue, J., TAkeshita, T., Kinjo, J. and Nohara, T. (1992) Triterpene glycoside from the seeds of Astragalus sinicus L. Chem. Pharm. Bull. 40: 3330-3333 https://doi.org/10.1248/cpb.40.3330
  15. Kitagawa, I., Wang, H. K., Saito, M., Takagi, A. and Yoshikawa, H. (1983) Astragalosides I, II and IV, acetyastragaloside I and isoastragalosides I and II. Chem. Pharm. Bull. 31: 698-708 https://doi.org/10.1248/cpb.31.698
  16. Ionkova, I. and Alfermann, W. (1990) Transformation of Astragalus species by Agrobacterium rhizogenes and their saponin production. Planta Med. 56: 634-635
  17. Li, C. Y. and Rhee, H. I. (2004) Antioxidant activity of Astragalus membranaceus extract. J. Agric. Sci. 15: 103-110
  18. Baek, N. I., Kim, Y. S., Kyung, J. S. and Park, K. H. (1996) Isolation of anti-hepatotoxic agent from the root of Astragalus membranacus. Kor. J. Pharmacogn. 27: 111-116
  19. Blois, M. S. (1958) Antioxidant determination by the use of a stable free radical. Nature 181: 1199-1200 https://doi.org/10.1038/1811199a0
  20. Birt, D. F., Hendrich, S. and Wang, W. Q. (2001) Dietary agents in cancer prevention: flavonoids and isoflavonoids. Pharmacol. Ther. 90: 157-177 https://doi.org/10.1016/S0163-7258(01)00137-1
  21. Lin, J. Y. and Tang, C. Y. (2007) Determination of total phnolic and flavonoid contents in selected fruits and vegetables, as well as their stimulatory effects on mouse splenocyte proliferation. Food Chem. 101: 140-147 https://doi.org/10.1016/j.foodchem.2006.01.014
  22. Choi, S. Y., Lim, S. H., Kim, J. S., Ha, T. Y., Kim, S. R., Kang, K. S. and Hwang, I. K. (2005) Evaluation of the estrogenic and antioxidant activity of some edible and medicinal plants. Korean J. Food Sci. Technol. 37: 549-556
  23. Lee, J. H. and Lee, S. R. (1994) Analysis of phenolic substances content in Korean plant food. Chem. Pharm. Bull. 26: 310-316
  24. Jung, M. J., Heo, S. I. And Wang, M. H. (2008) Free radical scavenging and total phenolic contents from methanolic extracts of Ulmus davidiana. Food Chem. 108: 482-487 https://doi.org/10.1016/j.foodchem.2007.10.081
  25. Pietta, P. G. (2000) Flavonoids as antioxidants. J. Nat. Prod. 63: 1035-1042 https://doi.org/10.1021/np9904509
  26. Shiddhuraju, P., Monhan, P. S. and Becker, K. (2002) Studies on the antioxidant activity of Indian laburnum (Cassia fistula L.): a reliminary assessment of crude extracts from stem bark, leaves, flowers and fruit pulp. Food Chem. 79: 61-67 https://doi.org/10.1016/S0308-8146(02)00179-6
  27. Kim, M. S., Choue, R. W., Chung, S. H. and Koo, S. J. (1998) Blood glucose lowering effects of Mulberry leaves and Silkworm extracts on mice fed with high-carbohydrate diet. Kor. J. Nut. 31: 117-125