Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
권호 표지

Antioxidant Activities of Flavonoids from the Leaves of Smilax china Linne

청미래덩굴 잎의 항산화 활성 성분

  • Cha, Bae-Cheon (Division of Animal Science and Biotechnology , Sangji University) ;
  • Lee, Eun-Hee (Division of Animal Science and Biotechnology , Sangji University)
  • 차배천 (상지대학교 생명자원과학대학 동물생명자원학부) ;
  • 이은희 (상지대학교 생명자원과학대학 동물생명자원학부)
  • Published : 2007.03.31
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Abstract

In this study, we have investigated the antioxidant activities of the leaves of Smilax china Linne in order to find the antioxidant substances which has an scavenging effect of reactive oxygen species (ROS) from natural products. As a result, EtOAc and n-BuOH extracts of the leaves of Smilax china Linne exhibited potent antioxidant effect on various antioxidant experiment. The major components of antioxidant activity were isolated from EtOAc and n-BuOH extracts of the leaves of Smilax china Linne. Their structure of compounds were identified as kaempferol-7-O-${\alpha}$-L-rhamnopyranoside and kaempferol-3,7-O-${\alpha}$-L-dirhamnopyranoside by spectroscopic evidence, respectively. Antioxidant activities were observed in kaempferrol-7-O-${\alpha}$-L-rhamnopyranoside at 23 ${\mu}g$ and in kaempferol-3,7-O-${\alpha}$-L-dirhamnopyranoside at 27 ${\mu}g$ as concentration of DPPH 50% reduction.

Keywords

References

  1. Ahmad, S. (1995) Oxidative stress and antioxidant defenses in biology. Chapman & Hall, New York
  2. Papa, S. and Skulachev, V. P. (1997) Reactive oxygen species, mitochondria, apoptosis and aging. Mol. Cell Biochem. 174: 305-319 https://doi.org/10.1023/A:1006873518427
  3. Fukuzawa, K. and Takaishi, Y. (1990) Antioxidants. J. Act. Oxyg. Free Rad. 1: 55-70
  4. Halliwell, B. (1991) Drug antioxidant effects. Drugs, 42: 569-605 https://doi.org/10.2165/00003495-199142040-00003
  5. Cerutti, P. A. (1994) Oxy-radicals and cancer. Lancet. 344: 862-863 https://doi.org/10.1016/S0140-6736(94)92832-0
  6. Halliwell, B. and Gutteridge, J. M. (1984) Lipid peroxidation, oxygen radical, cell damage and anti-oxidant therapy. Lancet. 23: 1396-1397
  7. Corl, M. M. (1974) Antioxidant activity of tocopherol and ascorbylpalmitate and their mode of action. JAOCS. 51: 321- 325 https://doi.org/10.1007/BF02633006
  8. Coleman, M. D., Fernandes, S. and Khanderia, L. A. (2003) A preliminary evaluation of a novel method to monitor a triple antioxidant combination (vitamin E, C and $\alpha$-lipoic acid) in diabetic volunteers using in vitro methaemoglobin formation. Environ. Toxicol. Pharmacol. 14: 69-75 https://doi.org/10.1016/S1382-6689(03)00027-9
  9. Monsen, E. R. (2000) Dietary reference intakes for the antioxidant nutrients : vitamin C, vitamin E, selenium, and carotenoids. J. Am. Diet. Assoc. 100: 637-640 https://doi.org/10.1016/S0002-8223(00)00189-9
  10. Ito, N., Fukushima, S., Hagiwara, A., Shibata, M. and Ogiso, T. (1983) Carcinogenicity of butylated hydroxyanisole on F 344 rats. J. Natl. Cancer Inst. 70: 343-352
  11. Branen, A. L. (1975) Toxicological and biochemistry of butylated hydroxyanisole and butylated hydroxytoluene. JAOCS. 52: 59-63 https://doi.org/10.1007/BF02901825
  12. Kolak, U., Ozturk, M., Ozgokce, F. and Ulubelen, A. (2006) Norditerpene alkaloids from Delphinium linearilobum and antioxidant activity. Phytochemistry, 67: 2170-2175 https://doi.org/10.1016/j.phytochem.2006.06.006
  13. Chung, Y. C., Chien, C. T., Teng, K. Y. and Chou, S. T. (2006) Antioxidative and mutagenic properties of Zanthoxylum ailanthoides Sieb & Zucc. Food Chem. 97: 418-425 https://doi.org/10.1016/j.foodchem.2005.05.019
  14. Lee, S. O., Kim, M. J., Kim, D. K. and Choi, H. J. (2005) Antioxidative activities of temperature-stepwise water extracts from Inonotus obliquus. Korean J. Soc. Food Sci. Nutr. 34: 139-147 https://doi.org/10.3746/jkfn.2005.34.2.139
  15. Yu, W., Zhao, Y. and Shu, B. (2004) The radical scavenging activities of radix puerariae isoflavonoids. Food Chem. 86: 525-529 https://doi.org/10.1016/j.foodchem.2003.09.005
  16. Kim, T. J. (1993) Alpine flowers of korea, 542. Kyo Hak Sa, Seoul
  17. Park, J. H., Kim, J. M. and Do, W. I. (2002) Pharmacognostical study on the To Bog Ryung. Kor. J. Pharmacogn. 33: 169-172
  18. Son, K. H., Seo, J. H., Lee, J. M., Kwon, S. J., Chang, S. Y. and Lee, K. S. (2001) Isolation and quantitative determination of dioscin from Smilacis Chinae Radix. Kor. J. Pharmacogn. 32: 153-156
  19. Choi, H. Y. (2004) Antimicrobial effect of ethanol extract of Smilax china leaf. Korean J. Sanitation, 19: 22-30
  20. Song, J. H., Kim, H. S., Kim, Y. G., Son, B. G., Choi, Y. W. and Kang, J. S. (1999) Antimicrobial activity of extract from Smilax china. J. Agrl. Tech. & Dev. Inst. 3: 1-6
  21. Jin, T. Y., Park, J. R. and Kim, J. H. (2004) Electron donating abilities, nitrite scavenging effects and antimicrobial activities of Smilax china leaf. J. Korean Soc. Food Sci. Nutr. 33: 621-625 https://doi.org/10.3746/jkfn.2004.33.4.621
  22. Uchiyama, M., Suzuki, Y. and Fukuzawa, K. (1968) Biochemical studies of physiological function of tocopheronolactone. 1. Yakugaku Zasshi, 88: 678-6834 https://doi.org/10.1248/yakushi1947.88.6_678
  23. Yoshikawa, M., Harada, E., Miki, A., Tsukamoto, K., Liang, S. Q., Yamahara, J. and Murakami, N. (1994) Antioxidant constituents from the fruit hulls of Mangosteen (Garcinia mangostana L.) originating in vietnam. Yakugaku Zasshi, 114: 129-133 https://doi.org/10.1248/yakushi1947.114.2_129
  24. Inatani, R., Nakatani, N. and Fuwa, H. (1983) Antioxidative effect of the constituents of rosemary (Rosemaries officinalis L.) and their derivatives. Agric. Biol. Chem. 47: 521-528 https://doi.org/10.1271/bbb1961.47.521
  25. Chen, C. W. and Ho, C. T. (1995) Antioxidant properties of polyphenols extracted from green and black teas. J. Food Lipids, 2: 35-46 https://doi.org/10.1111/j.1745-4522.1995.tb00028.x
  26. Lim, D. K., Choi, U. and Shin, D. H. (1996) Antioxidative of some solvent extract from Caesalpinia sappan L. Korean J. Food Sci. Technol. 28: 77-82
  27. Choe, S. Y. and Yang, K. H. (1982) Toxicological studies of antioxidants butylated hydroxytoluene (BHT) and butylated hydroxyanisole (BHA). Korean J. Food Sci. Technol. 14: 283-288
  28. Sharaf, M., EL-Ansari, M. A. and Saleh, N. A. M. (1997) Flavonoids of four Cleome and three Capparis species. Biochem. Syst. Ecol. 25: 161-166 https://doi.org/10.1016/S0305-1978(96)00099-3
  29. Ozden, S., Durust, N., Toki, K., Saito, N. and Honda, T. (1998) Acylated kaempferol glycosides from the flowers of Delphinium formosum. Phytochemistry, 49: 241-245 https://doi.org/10.1016/S0031-9422(97)01044-3
  30. EI-Sayed, N. H., Awaad, A. S., Hifnawy, M. S. and Mabry, T. J. (1999) A flavonol triglycoside from Chenopodium murale. Phytochemistry, 51: 591-593 https://doi.org/10.1016/S0031-9422(99)00038-2
  31. Iwashina, T., Matsumoto, S., Nishida, M. and Nakaike, T. (1995) New and rare flavonol glycosides from Asplenium trichomanes- ramosum as stable chemotaxonomic markers. Biochem. Syst. Ecol. 23: 283-290 https://doi.org/10.1016/0305-1978(94)E0076-R
  32. Perez-Castorena, A., Castro, A. and De Vivar, A. R. (1997) A dirhamnopyranoside from Psacalium megaphyllum. Phytochemistry, 46: 1297-1299 https://doi.org/10.1016/S0031-9422(97)80032-5
  33. Moreno, A., Martin-Cordero, C., Iglesias-Guerra, F. and Toro, M. V. (2002) Flavonoids from Dorycnium rectum. Biochem. Syst. Ecol. 30: 73-74 https://doi.org/10.1016/S0305-1978(01)00070-9