Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
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Production and Embryo Transfer of In Vivo Embryos by Repeated Superovulation Treatment of Hanwoo Cattle

한우의 반복 과배란 처리에 의한 체내 수정란의 생산과 이식

  • Shin, Sang-Min (College of Veterinary Medicine, Chonbuk National University) ;
  • Kim, Yong-Jun (College of Veterinary Medicine, Chonbuk National University) ;
  • Lee, Hae-Lee (Livestock Breeding Starion, Jeonbuk Livestock Development & Research Institute) ;
  • Shin, Dong-Su (Livestock Breeding Starion, Jeonbuk Livestock Development & Research Institute) ;
  • Kim, Yong-Su (Livestock Breeding Starion, Jeonbuk Livestock Development & Research Institute) ;
  • Kim, Sue-Hee (College of Veterinary Medicine, Chonbuk National University) ;
  • Lee, Young-Jun (College of Veterinary Medicine, Chonbuk National University)
  • 신상민 (전북대학교 수의과대학) ;
  • 김용준 (전북대학교 수의과대학) ;
  • 이해리 (전라북도 축산위생연구소 종축시험소) ;
  • 신동수 (전라북도 축산위생연구소 종축시험소) ;
  • 김용수 (전라북도 축산위생연구소 종축시험소) ;
  • 김수희 (전북대학교 수의과대학) ;
  • 이영준 (전북대학교 수의과대학)
  • Published : 2009.03.31
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Abstract

This study was performed to investigate the possibility of repeated superovulation treatment at interval from 27 days to 41 days in Hanwoo cattle and to compare with superovulation effect between doses of FSH 200 mg and FSH 400 mg. Different doses of FSH (200 mg or 400 mg) were injected at Day 8 after controlled internal drug release (CIDR) treatment for superovulation of Hanwoo donors following CIDR treatment (Day 8 after the estrus). Superovulation was repeated four times for one donor and number of corpus luteum (CL), number of embryos, number of transferable embryos and pregnancy rate after embryo transfer (ET) were investigated. 5 cows were used for each FSH treatment (10 cows in total). Average number of CL were $10.16{\pm}3.85$ and $11.56{\pm}2.35$ for the donors treated with FSH 200mg and FSH 400mg, respectively. Average number of embryos collected were $8.85{\pm}4.05$ and $8.30{\pm}1.73$ for the donors treated with FSH 200 mg and FSH 400 mg, respectively. Average number of transferable embryos were $5.48{\pm}2.45$ and $4.58{\pm}2.23$ for the donors treated with FSH 200 mg and FSH 400 mg, respectively. The pregnancy rate following ET with embryos collected from 200 mg FSH treated donors and 400 mg FSH treated donors were 61.9% and 53.8% respectively. The numbers of embryos tended to be decreased as the numbers of repeat of superovulation were elapsed. These results indicated that superovulation treatment by about a month to Hanwoo donors is usable and 200 mg of FSH is preferable for simple FSH treatment following CIDR treatment.

Keywords

References

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