Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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LC-MS/MS Profiling-Based Secondary Metabolite Screening of Myxococcus xanthus

  • Kim, Ji-Young (Functional Metabolomics Laboratory, Division of Bioscience and Biotechnology, Konkuk University) ;
  • Choi, Jung-Nam (Functional Metabolomics Laboratory, Division of Bioscience and Biotechnology, Konkuk University) ;
  • Kim, Pil (Department of Biotechnology, Catholic University of Korea) ;
  • Sok, Dai-Eun (Chungnam National University) ;
  • Nam, Soo-Wan (Department of Biotechnology and Bioengineering/Department of Biomaterial Control, Dong-Eui University) ;
  • Lee, Choong-Hwan (Functional Metabolomics Laboratory, Division of Bioscience and Biotechnology, Konkuk University)
  • Received : 2007.11.19
  • Accepted : 2008.07.31
  • Published : 2009.01.31
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Abstract

Myxobacteria, Gram-negative soil bacteria, are a well-known producer of bioactive secondary metabolites. Therefore, this study presents a methodological approach for the high-throughput screening of secondary metabolites from 4 wild-type Myxococcus xanthus strains. First, electrospray ionization mass spectrometry (ESI-MS) was performed using extracellular crude extracts. As a result, 22 metabolite peaks were detected, and the metabolite profiling was then conducted using the m/z value, retention time, and MS/MS fragmentation pattern analyses. Among the peaks, one unknown compound peak was identified as analogous to the myxalamid A, B, and C series. An analysis of the tandem mass spectrometric fragmentation patterns and HR-MS identified myxalamid K as a new compound derived from M. xanthus. In conclusion, LC-MS/MS-based chemical screening of diverse secondary metabolites would appear to be an effective approach for discovering unknown microbial secondary metabolites.

Keywords

References

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