Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Enhancement of Immuno-modulatory of Centella asiatica L. Urban with Edible Polymer through Nano-encapsulation Process

병풀 추출물의 식용 나노입자화를 통한 면역 활성 증진

  • Ha, Ji-Hye (College of Bioscience and Biotechnology, Kangwon National Univ.) ;
  • Kwon, Min-Chul (College of Bioscience and Biotechnology, Kangwon National Univ.) ;
  • Kim, Young (College of Bioscience and Biotechnology, Kangwon National Univ.) ;
  • Jeong, Seung-Seop (College of Bioscience and Biotechnology, Kangwon National Univ.) ;
  • Jeong, Myoung-Hoon (College of Bioscience and Biotechnology, Kangwon National Univ.) ;
  • Hwang, Baik (Department of Biology, Chonnam National Univ.) ;
  • Lee, Hyeon-Yong (College of Bioscience and Biotechnology, Kangwon National Univ.)
  • 하지혜 (강원대학교 BT특성화학부대학) ;
  • 권민철 (강원대학교 BT특성화학부대학) ;
  • 김영 (강원대학교 BT특성화학부대학) ;
  • 김승섭 (강원대학교 BT특성화학부대학) ;
  • 정명훈 (강원대학교 BT특성화학부대학) ;
  • 황백 (전남대학교 생물학과) ;
  • 이현용 (강원대학교 BT특성화학부대학)
  • Published : 2009.08.30
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Abstract

Phosphatidylcholine was used to encapsulate aqueous extracts of Centella asiatica, and its biological activity was compared with another aqueous extracts. Nanoparticle of C. asiatica was made by encapsulation to w/o type spherical liposome which of aqueous extracts seized with oil phase as 78.2 nm average diameter. Cytotoxicity of the nanoparticle was measured on human skin fibroblast cells, CCD-986sk, and showed lower cytotoxicity on 1.0 mg/$m{\ell}$ of highest concentration as 28% than that of another extracts. The nanoparticle showed the highest promotion of human B and T cell growth up to 138% and 135%, respectively, compared to the control. and the NK cell growth was promoted up to 8% higher than the control in proportion to secretion of IL-6 and TNF-$\alpha$ from immune cell growth. Also nanoparticle showed highest inhibition activity of hyaluronidase on 1.0 mg/$m{\ell}$ of highest concentration as 60.5%. It seems that because of enhanced biological application of aqueous extracts on cell through nano-encapsulation process.

Keywords

References

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