Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Detection of Pediococci in Kimchi Using Pediococci Selective Medium

Pediococci 선택배지를 이용한 김치 유래 Pediococci 검출

  • Lee, Myeong-Jae (Department of Food Science and Biotechnology, Kyonggi University) ;
  • Lee, Jong-Hoon (Department of Food Science and Biotechnology, Kyonggi University)
  • 이명재 (경기대학교 식품생물공학과) ;
  • 이종훈 (경기대학교 식품생물공학과)
  • Published : 2009.09.28
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Abstract

Pediococci selective medium (PSM) supplemented with ampicillin (A) reported as valid for the detection and enumeration of pediococci included in foods and animal feed was evaluated for the selective detection of the genus Pediococcus in kimchi. PSM is based on the complex basal medium MRS supplemented with cysteine hydrochloride, vancomycin, novobiocin, and nystatin. In the medium evaluation with known species, the growth inhibition of leuconostocs, Pediococcus pentosaceus, Lactobacillus casei, Lactobacillus curvatus, Oenococcus oeni, and Streptococcus thermophilus was not confirmed. In the application of kimchi samples on the selective medium, leuconostocs, P. pentosaceus, Weissella koreensis, Lb. curvatus, Lactobacillus brevis, and Lactobacillus sakei were detected. PSM+A was proved to be not applicable for the detection of pediococci in kimchi.

분자생물학적 방법에 의한 김치유산균 연구의 진행으로 Leuconostoc, Lactobacillus, Weissella 속 유산균의 김치발효 과정 중 생육에 대한 새로운 결과들이 도출되었지만, Pediococcus속의 생육에 대한 새로운 결과는 거의 보고되지 않았다. 본 연구에서는 김치에 존재하는 Pediococcus 속 유산균의 존재에 대한 재조명을 위하여 ampicillin (A)의 첨가로 pediococci의 선발 유효성이 향상된 것으로 보고된 pediococci selective medium(PSM)+A를 이용하여 pediococci 선택적 검출의 유효성을 검토한 결과, 기존에 보고된 결과와는 달리 Pediococcus pentosaceus 외에도 leuconostocs, Lactobacillus casei, Lactobacillus curvatus, Oenococcus oeni, Streptococcus thermophilus에 대한 생육저해가 발생하지 않았다. PSM+A 한천배지를 이용하여 김치에 생육하는 미생물을 검출한 경우, leuconostocs, P. pentosaceus, Weissella koreensis, Lb. curvatus, Lactobacillus brevis, Lactobacillus sakei가 생육하여 김치와 같은 발효침채류의 발효에 관여하는 pediococci의 선택적 선발에는 PSM+A가 유효하지 않은 것으로 확인되었다.

Keywords

References

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