Food Science and Biotechnology

Korean Society of Food Science and Technology (한국식품과학회)
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Antioxidant and NO-scavenging Activities of Acanthopanax senticosus var. subinermis Leaf Extracts Prepared Using Ethanol and Extrusion Processing

  • Lee, Sung-Hee (Department of Food and Nutrition, Brain Korea 21 Project, Yonsei University, College of Human Ecology) ;
  • Oh, Hea-Young (Department of Food and Nutrition, Brain Korea 21 Project, Yonsei University, College of Human Ecology) ;
  • Leem, Jae-Yoon (Department of Pharmacy, College of Pharmacy, Woosuk University) ;
  • Yoon, Sun (Department of Food and Nutrition, Brain Korea 21 Project, Yonsei University, College of Human Ecology)
  • Published : 2009.10.31
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Abstract

Acanthopanax senticosus has been used in East Asia as a traditional medicine. The purpose of this study is the investigation of an extraction method for A. senticosus var subinermis (ASvS) leaves to improve their antioxidant and anti-inflammatory activities. Six extracts were prepared: extracted with water (W), ethanol (Eth), water or ethanol after ultra high pressure (WP, EthP), and water or ethanol after an extrusion process (WEx, EthEx). Diphenylpicrylhydrazyl (DPPH) radical scavenging activity and ferric reducing ability showed that all extracts had a significant antioxidant activity. In anti-inflammatory activities, Eth and EthEx significantly inhibited the release of lipopolysaccharide (LPS)-induced nitric oxide (NO), tumor necrosis factor (TNF)-${\alpha}$, and interleukin (IL)-$1{\beta}$ independent of cell viability in RAW 264.7 macrophage cells. Inducible NO synthase (iNOS) protein was significantly decreased by EthEx. These findings present that Eth and EthEx extracts of ASvS leaves have anti-inflammatory activities, and EthEx extract suppresses LPS-induced NO through the down-regulation of iNOS and pro-inflammatory cytokines.

Keywords

References

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