Journal of Oriental Neuropsychiatry (동의신경정신과학회지)

The Korean Society of Oriental Neuropsychiatry (대한한방신경정신과학회)
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Up-and-Down Procedure(UDP) Determinations of Acute Oral Toxicity of LMK02-Jangwonhwan in SD Rats

Sprague-Dawley 랫드를 이용한 Up & Down 법 (UDP)에 의한 LMK02의 단회 경구투여 독성시험

  • Kang, Hyung-Won (Dept. of Neuropsychiatry, College of Oriental Medicine, Won-Kwang University) ;
  • Kwon, Yeong-Mi (Dept. of Diagnostic Radiology, College of Oriental Medicine Won-Kwang University) ;
  • Lee, Sang-Won (National Institute of Horticultural and Herbal Science) ;
  • Kim, Ji-Hwon (Dept. of Neuropsychiatry, College of Oriental Medicine, Won-Kwang University) ;
  • Lee, Hyo-Gyung (Dept. of Neuropsychiatry, College of Oriental Medicine, Won-Kwang University) ;
  • Jang, Hyun-Ho (Dept. of Neuropsychiatry, College of Oriental Medicine, Won-Kwang University) ;
  • Park, Bo-Ra (Dept. of Neuropsychiatry, College of Oriental Medicine, Won-Kwang University) ;
  • Lyu, Yeoung-Su (Dept. of Neuropsychiatry, College of Oriental Medicine, Won-Kwang University)
  • 강형원 (원광대학교 한의과대학 한방신경정신과교실) ;
  • 권영미 (원광대학교 전주한방병원 방사선과교실) ;
  • 이상원 (인삼특용작목반) ;
  • 김지훤 (원광대학교 한의과대학 한방신경정신과교실) ;
  • 이효경 (원광대학교 한의과대학 한방신경정신과교실) ;
  • 장현호 (원광대학교 한의과대학 한방신경정신과교실) ;
  • 박보라 (원광대학교 한의과대학 한방신경정신과교실) ;
  • 류영수 (원광대학교 한의과대학 한방신경정신과교실)
  • Received : 2010.08.10
  • Accepted : 2010.09.04
  • Published : 2010.09.30
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Abstract

Objectives : The oriental medicine Jangwonhwan originally described in the Korean medical text, DonguiBogam(amnesia chapter). Recently, a modified formula of Jangwonhwan(LMK02-Jangwonhwan), was shown to reduce $\beta$-amyloid deposition in the brain of Tg-APPswe/PS1dE9 mouse model for Alzheimer's disease. This experiment aimed to investigate the acute oral toxicity of LMK02 in SD rats by up-and-down procedure determinations. Methods : Quality control of tablet form of LMK02 was established by estimating indicative components, Ginsenoside Rg3 of Red Ginseng and Decursin of Angelicagigas Nakai. The toxicity of LMK02 was investigated in 6 week old, specific pathogen free(SPF), Sprageu-Dawley rats. 3 female rats received 5,000 mg/10 ml/kg of test substance and their death rate, clinical sings, weight changes and autopsy findings had been observed for 2 weeks. Results : Any specific symptoms or death were resulted in this experiment. No significant changes in rats' weight. No significant differences in atopsy. Conclusions : The minimum lethal dose(MLD) of LMK02 for female Sprauge-Dawley rats were more than 5,000mg/kg in this experiment.

Keywords

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