Horticultural Science & Technology (원예과학기술지)

Korean Society of Horticultural Science (한국원예학회)
권호 표지

Evaluation of TYLCV-resistant Tomato Germplasm Using Molecular Markers

분자표지를 이용한 TYLCV 저항성 토마토 유전자원 평가

Park, Young-Hoon;Kim, Kwang-Hwan;Choi, Young-Mi;Choi, Hak-Soon;Chae, Young;Park, Kwon-Seo;Chung, Sang-Min
박영훈;김광환;최영미;최학순;채영;박권서;정상민

  • Published : 20100200
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Abstract

Tomato yellow leaf curl virus (TYLCV) infection causes serious economic losses in tomato production world-wide, and the development of new domestic cultivars with high levels of resistance to TYLCV is required. In this study, major advanced breeding lines carrying TYLCV-resistance loci, Ty-1, Ty-2, Ty-3, and Ty-3a were collected from overseas breeding programs, and their allelic composition of each resistance locus were confirmed by using public PCR-based DNA markers. Perfect matches between marker genotypes and the resistance indicated a feasibility of marker-assisted selection (MAS). In addition, genetic relationship of the TYLCV-resistant germplasm with commercial hybrids broadly grown in South Korea and other breeding lines were evaluated by AFLPs. A very close genetic background among these tomato germplasm revealed by the genetic similarity coefficient (0.87 to 1.00) and UPGMA implied the easiness of a rapid incorporation of the resistance genes. Phenetic tree showed a close relationship between TYLCV-resistant CLN lines and general type commercial hybrids, while Gc lines formed an independent group distinguished from both general and cherry type cultivars. Pyramiding TYLCV-resistance alleles using the resistant germplasm and DNA markers introduced in this report will greatly improve the breeding process for high levels of TYLCV-resistance. For accurate evaluation of the TYLCV-resistance, however, a reliable method of bio-assay still remains to be established.

토마토 황화잎말림바이러스(tomato yellow leaf curly virus, TYLCV)병은 세계전역에 걸쳐 토마토 생산에 막대한 손실을 입히고 있으며, 국내에서도 피해 농가가 속출하고 있어, 강한 저항성을 지닌 국내 품종의 개발이 매우 시급하다. 본 연구에서는 현재까지 알려진 주요 TYLCV 저항성 유전자인 Ty-1, Ty-2, Ty-3, Ty-3a를 지니는 저항성 계통을 해외로부터 도입하여 계통이 지니는 각 저항성 유전자간 대립유전자의 조성을 DNA 분자표지를 이용하여 분석한 결과, 분자표지의 유전형과 저항성 표현형간의 완전한 일치를 관찰할 수 있었다. 또한 AFLP을 통한 유연관계분석에서 TYLCV 저항성 유전자원과 국내 시판 품종 및 육성계통간 유전적 배경이 상당히 유사한 것으로 드러나, 본 유전자원을 국내 육종재료로 활용하는 것이 매우 용이하리라 판단되었다. 본 연구에서 소개된 TYLCV 저항성 도입계통들과 분자표지를 이용하여 다양한 저항성 유전자들을 집적시킴으로써, 높은 수준의 TYLCV 저항성을 지닌 국내 품종개발이 단시간에 가능하리라 생각된다. 하지만, 보다 정확한 저항성 판별을 위해서는 효율적인 생물검정기술의 확보가 국내 품종육종에 절실하다.

Keywords

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