BMB Reports

Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
권호 표지
DOI QR코드

DOI QR Code

Inhibition of the expression on MMP-2, 9 and morphological changes via human fibrosarcoma cell line by 6,6'-bieckol from marine alga Ecklonia cava

  • Zhang, Chen (Department of Chemistry, Pukyong National University) ;
  • Li, Yong (Marine Bioprocess Research Center, Pukyong National University) ;
  • Shi, Xiujuan (Key Laboratory of Molecular Enzymology and Enzyme Engineering of Ministry Education, Jilin University) ;
  • Kim, Se-Kwon (Department of Chemistry, Pukyong National University)
  • Published : 2010.01.31
Download PDF
⟨ Previous Next ⟩

Abstract

Matrix Metalloproteinases (MMPs) are a family of zinc-endopeptidases which can degrade extracellular matrix (ECM) components and play important roles in a variety of biological and pathological processes. 6,6'-bieckol isolated and characterized from an edible marine brown alga Ecklonia cava (EC), according to the comprehensive spectral analysis of MS and NMR data. Here the influence of 6,6'-bieckol on expressions of MMPs was examined by zymography and western blot analysis via human fibrosarcoma cell line (HT1080). It is shown that 6,6'-bieckol significantly down regulated the expressions of MMP-2 and -9 in dose-dependent manner. The influence of 6,6'-bieckol on the cell viability and cell behavior of HT1080 cells were also investigated, our dates shown that it suppressed the migration and 3D culture in HT1080 cells. Meanwhile, we explored several signal pathways which may contribute to this process, and found the suppressing of MMPs expressions in HT1080 cells might be due to the suppression of NF-${\kappa}B$ signal pathway.

Keywords

References

  1. Pasco, S., Brassart, B., Ramont, L., Maquart, F. X. and Monboisse, J. C. (2005) Control of melanoma cell invasion by type IV collagen. Cancer Detect. Prev. 29, 260-266 https://doi.org/10.1016/j.cdp.2004.09.003
  2. Johnson, L. L., Dyer, R. and Hupe, D. J. (1998) Matrix metalloproteinases. Curr. Opin. Chem. Biol. 2, 466-471 https://doi.org/10.1016/S1367-5931(98)80122-1
  3. Woessner, J. F., Jr. (1991) Matrix metalloproteinases and their inhibitors in connective tissue remodeling. Faseb J. 5, 2145-2154 https://doi.org/10.1096/fasebj.5.8.1850705
  4. Baker, A. H., Edwards, D. R. and Murphy, G. (2002)Metalloproteinase inhibitors: biological actions and therapeutic opportunities. J. Cell Sci. 115, 3719-3727 https://doi.org/10.1242/jcs.00063
  5. Ashley, R. A. (1999) Clinical trials of a matrix metalloproteinase inhibitor in human periodontal disease. Ann. NY. Acad. Sci. 878, 335-346 https://doi.org/10.1111/j.1749-6632.1999.tb07693.x
  6. Ali, M. A. and Schulz, R. (2009) Activation of MMP-2 as a key event in oxidative stress injury to the heart. Front. Biosci. 14, 699-716
  7. Sen, T., Moulik, S., Dutta, A., Choudhury, P. R., Banerji, A.,Das, S., Roy, M. and Chatterjee, A. (2009) Multifunctional effect of epigallocatechin-3-gallate (EGCG) in downregulation of gelatinase-A (MMP-2) in human breast cancer cell line MCF-7. Life Sci. 84, 194-204 https://doi.org/10.1016/j.lfs.2008.11.018
  8. Das, S., Banerji, A., Frei, E. and Chatterjee, A. (2008)Rapid expression and activation of MMP-2 and -9 upon exposure of human breast cancer cells (MCF-7) to fibronectin in serum free medium. Life Sci. 82, 467-476 https://doi.org/10.1016/j.lfs.2007.12.013
  9. Westermarck, J. and K$\ddot{a}$h$\ddot{a}$ri, V. M. (1999) Regulation of matrix metalloproteinase expression in tumor invasion. Faseb J. 13, 781-792 https://doi.org/10.1096/fasebj.13.8.781
  10. John, A. and Tuszynski, G. (2001) The role of matrix metalloproteinases in tumor angiogenesis and tumor metastasis. Pathol. Oncol. Res. 7, 14-23 https://doi.org/10.1007/BF03032599
  11. Yang, B. C., Zhang, X. M., Bao, X. L., Lv, Y., Zhang, J. and Guo, S. T. (2008) Glycopeptide derived from soybean $\beta$-conglycinin inhibits the adhesion of Escherichia coli and Salmonella to human intestinal cells. Food Res. Int. 41, 594-599 https://doi.org/10.1016/j.foodres.2008.04.009
  12. Yuan, Y. V. and Walsh, N. A. (2006) Antioxidant and antiproliferative activities of extracts from a variety of edible seaweeds. Food Chem. Toxicol. 44, 1144-1150 https://doi.org/10.1016/j.fct.2006.02.002
  13. Kim, M. M., Ngo, D. N., Lee, S. H., Jung, W. K., Li, Y.,Qian, Z. J. and Kim, S. K. (2008) Inhibitory effect of phloroglucinol on chronic inflammation. J. Biotechnol. 136, 577-578
  14. Artan, M., Li, Y., Karadeniz, F., Lee, S. H., Kim, M. M. and Kim, S. K. (2008) Anti-HIV-1 activity of phloroglucinol derivative, 6,6'-bieckol, from Ecklonia cava. Bioorg. Med Chem. 16, 7921-7926 https://doi.org/10.1016/j.bmc.2008.07.078
  15. Slade P. J. (1992) Monitoring Listeria in the food production environment. II. Identification techniques. Food Res. Int. 25, 203-214 https://doi.org/10.1016/0963-9969(92)90139-V
  16. Kang, H. S., Chung, H. Y., Kim, J. Y., Son, B. W., Jung, H.A. and Choi, J. S. (2004) Inhibitory phlorotannins from the edible brown alga Ecklonia stolonifera ontotal reactive oxygen species (ROS) generation. Arch. Pharm. Res. 27, 194-198 https://doi.org/10.1007/BF02980106
  17. Seo, E., Kim, S. and Jho, E. H. (2009) Induction of cancer cell-specific death via MMP2 promoterdependent Bax expression. J. Biochem. Mol.Biol. 42, 217-222
  18. Hotary, K. B., Allen, E. D., Brooks, P. C., Datta, N. S.,Long, M. W. and Weiss, S. J. (2003) Membrane Type I matrix metalloproteinase usurps tumor growth control imposed by the three-dimensional extracellular matrix. Cell 114, 33-45 https://doi.org/10.1016/S0092-8674(03)00513-0
  19. Le, Q. T., Li, Y., Qian, Z. J., Kim, M. M. and Kim, S. K.(2009) Inhibitory effects of polyphenols isolated from marine alga Ecklonia cava on histamine release. Process Biochem. 44, 168-176 https://doi.org/10.1016/j.procbio.2008.10.002
  20. Newby, A. C. (2007) Metalloproteinases and vulnerable atherosclerotic plaques. Trends Cardiovasc. Med. 17, 253-258 https://doi.org/10.1016/j.tcm.2007.09.001
  21. Shiozawa, S. and Tsumiyama, K. (2009) Pathogenesis of rheumatoid arthritis and c-Fos/AP-1. Cell Cycle. 8, 1539-1543 https://doi.org/10.4161/cc.8.10.8411
  22. Stricklin, G. P. and Welgus, H. G. (1983) Human skin fibroblast collagenase inhibitor. Purification and biochemical characterization. J. Biol. Chem. 258, 12252-12258
  23. Yoon, S. O., Park, S. J., Yoon, S. Y., Yun, C. H. and Chung,A. S. (2002) Sustained production of H_{2}O_{2} activates pro-matrix metalloproteinase-2 through receptor tyrosine kinase/phosphatidylinositol 3-kinase/NF-$\kappa$B pathway. J. Biol. Chem. 277, 30271-30282 https://doi.org/10.1074/jbc.M202647200
  24. Moon, S. K., Kim, H. M. and Kim, C. H. (2004) PTEN induces G1 cell cycle arrest and inhibits MMP-9 expression via the regulation of NF-kappaB and AP-1 in vascular smooth muscle cells. Arch. Biochem. Biophys. 421, 267-276 https://doi.org/10.1016/j.abb.2003.11.007
  25. Rajapakse, N., Kim, M. M., Mendis, E., Huang, R. and Kim, S. K. (2006) Carboxylated chitooligosaccharides (CCOS) inhibit MMP-9 expression in human fibrosarcoma cells via down-regulation of AP-1. Biochim. Biophys. Acta-Gen. Subj. 1760, 1780-1788 https://doi.org/10.1016/j.bbagen.2006.08.021
  26. Chiu, Y. H., Zhao, M. and Chen, Z. J. (2009) Ubiquitin in NF-kappaB signaling. Chem. Rev. 109, 1549-1560 https://doi.org/10.1021/cr800554j
  27. Beg, A. A. and Baltimore, D. (1996) An essential role for NF-kappaB in preventing TNF-alpha-induced cell death. Science 274, 782-784 https://doi.org/10.1126/science.274.5288.782
  28. Stier, S., Totzke, G., Gruewald, E., Neuhaus, T., Fronhoffs, S.,Schoneborn, S., Vetter, H. and Ko, Y. (2005) Identification of $p54^{nrb}$ and the 14-3-3 Protein HS1 as TNF-$\alpha$-Inducible Genes Related to Cell Cycle Control and Apoptosis in Human Arterial Endothelial Cells. J. Biochem. Mol.Biol. 38, 447-456 https://doi.org/10.5483/BMBRep.2005.38.4.447
  29. Overall, C. M. and López-Otín, C. (2002) Strategies for MMP inhibition in cancer: innovations for the post-trial era. Nat. Rev. Cancer 2, 657-672 https://doi.org/10.1038/nrc884
  30. Shibata, T., Fujimoto, K., Nagayama, K., Yamaguchi, K. and Nakamura, T. (2002) Inhibitory activity of brown algal phlorotannins against hyaluronidase. Int. J. Food Sci. Technol. 37, 703-709 https://doi.org/10.1046/j.1365-2621.2002.00603.x
  31. Krebs, K. G., Heusser, D. and Wimmer, H. (1969) Thinlayer chromatography: a laboratory handbook, 2nd ed., pp.854–909, Springer-Verlag, Berlin, Germany
  32. Lee, K. J., Kim, J. Y., Choi, J. H., Kim, H. G., Chung, Y. C.,Roh, S. H. and Jeong, H. G. (2006) Inhibition of tumor invasion and metastasis by aqueous extract of the radix of Platycodon grandiflorum. Food Chem. Toxicol. 44, 1890- 1896 https://doi.org/10.1016/j.fct.2006.06.009
  33. Frisk, T., Rydholm, S., Andersson, H., Stemme, G. and Brismar, H. (2005) A concept for miniaturized 3-D cell culture using an extracellular matrix gel. Electrophoresis. 26, 4751-4758 https://doi.org/10.1002/elps.200500478
  34. Frisk, T., Rydholm, S., Liebmann, T., Svahn, H. A., Stemme, G. and Brismar, H. (2007) A microfluidic device for parallel 3-D cell cultures in asymmetric environments. Electrophoresis. 28, 4705-4712 https://doi.org/10.1002/elps.200700342
  35. Jackson, M. T., Smith, M. M., Smith, S. M., Jackson, C. J., Xue, M. and Little, C. B. (2009) Activation of cartilage matrix metalloproteinases by activated protein C. Arthritis Rheum. 6, 780-791
  36. Kim, J. H., Kim, J., Jang , Y. S. and Chung, G. H. (2006)Germinal Center-independent Affinity Maturation in Tumor Necrosis Factor Receptor 1-deficient Mice. J. Biochem. Mol. Biol. 39, 586-594 https://doi.org/10.5483/BMBRep.2006.39.5.586

Cited by

  1. Trafficking and secretion of matrix metalloproteinase-2 in olfactory ensheathing glial cells: A role in cell migration? vol.59, pp.5, 2011, https://doi.org/10.1002/glia.21146
  2. Inhibitory Effects of Glycoprotein Isolated from Laminaria japonica on Lipopolysaccharide-Induced Pro-Inflammatory Mediators in BV2 Microglial Cells vol.76, pp.7, 2011, https://doi.org/10.1111/j.1750-3841.2011.02287.x
  3. Abalone Haliotis discus hannai Intestine Digests with Different Molecule Weights Inhibit MMP-2 and MMP-9 Expression in Human Fibrosarcoma Cells vol.15, pp.2, 2012, https://doi.org/10.5657/FAS.2012.0137
  4. Advanced adipose-derived stem cell protein extracts with antioxidant activity modulates matrix metalloproteinases in human dermal fibroblasts vol.34, pp.2, 2012, https://doi.org/10.1016/j.etap.2012.04.010
  5. Apoptosis induction of U937 human leukemia cells by diallyl trisulfide induces through generation of reactive oxygen species vol.19, pp.1, 2012, https://doi.org/10.1186/1423-0127-19-50
  6. HER2 induces expression of leptin in human breast epithelial cells vol.45, pp.12, 2012, https://doi.org/10.5483/BMBRep.2012.45.12.164
  7. Three-dimensional (3D) culture in sarcoma research and the clinical significance vol.9, pp.3, 2017, https://doi.org/10.1088/1758-5090/aa7fdb
  8. Applications for Marine Resources in Cosmetics vol.4, pp.3, 2017, https://doi.org/10.3390/cosmetics4030035
  9. Application of marine biomaterials for nutraceuticals and functional foods vol.21, pp.3, 2012, https://doi.org/10.1007/s10068-012-0081-6
  10. Marine algae-mediated synthesis of gold nanoparticles using a novel Ecklonia cava vol.37, pp.8, 2014, https://doi.org/10.1007/s00449-014-1131-7
  11. Bioactive properties and potentials cosmeceutical applications of phlorotannins isolated from brown seaweeds: A review vol.162, 2016, https://doi.org/10.1016/j.jphotobiol.2016.06.027
  12. Protective effect of porphyra-334 on UVA-induced photoaging in human skin fibroblasts vol.34, pp.3, 2014, https://doi.org/10.3892/ijmm.2014.1815
  13. The potential of seaweed as a source of drugs for use in cancer chemotherapy vol.26, pp.5, 2014, https://doi.org/10.1007/s10811-014-0245-2
  14. Tetraspanin CD9 Promotes the Invasive Phenotype of Human Fibrosarcoma Cells via Upregulation of Matrix Metalloproteinase-9 vol.8, pp.6, 2013, https://doi.org/10.1371/journal.pone.0067766
  15. From Marine Origin to Therapeutics: The Antitumor Potential of Marine Algae-Derived Compounds vol.9, pp.1663-9812, 2018, https://doi.org/10.3389/fphar.2018.00777