Journal of Environmental Health Sciences (한국환경보건학회지)

Korean Society of Environmental Health (한국환경보건학회)
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Determination of Total Aflatoxins in Foods by Parallelism of ELISA and LC/MS/MS

ELISA-LC/MS/MS 병행에 의한 식품 중 aflatoxins 분석

  • Kim, Kyeong-Yeol (Division of Applied Life Science(BK 21 program), Graduate School, Gyeongsang National University) ;
  • Nam, Min-Ji (Division of Applied Life Science(BK 21 program), Graduate School, Gyeongsang National University) ;
  • Nam, Bo-Ram (Division of Applied Life Science(BK 21 program), Graduate School, Gyeongsang National University) ;
  • Ryu, Hee-Jung (Division of Applied Life Science(BK 21 program), Graduate School, Gyeongsang National University) ;
  • Song, Jeong-Eon (Division of Applied Life Science(BK 21 program), Graduate School, Gyeongsang National University) ;
  • Shim, Won-Bo (Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Lee, Soo-Hyung (Microbial Safety Division, National Academy of Agricultural Science and Technology, Rural Development Administration) ;
  • Chung, Duck-Hwa (Division of Applied Life Science(BK 21 program), Graduate School, Gyeongsang National University)
  • 김경열 (경상대학교 응용생명과학부(BK 21 program)) ;
  • 남민지 (경상대학교 응용생명과학부(BK 21 program)) ;
  • 남보람 (경상대학교 응용생명과학부(BK 21 program)) ;
  • 류희정 (경상대학교 응용생명과학부(BK 21 program)) ;
  • 송정언 (경상대학교 응용생명과학부(BK 21 program)) ;
  • 심원보 (경상대학교 농업생명과학연구원) ;
  • 이수형 (농촌진흥청 국립농업과학원 유해생물과) ;
  • 정덕화 (경상대학교 응용생명과학부(BK 21 program))
  • Received : 2009.12.17
  • Accepted : 2010.02.01
  • Published : 2010.02.28
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Abstract

High performance liquid chromatography (HPLC) and liquid chromatography mass spectrometry (LC/MS) have been widely used to quantify aflatoxins in food, but these methods are expensive, time-consuming, unsuitable for analysis of the routine screening of large sample numbers and require derivatization and high level techniques to perform. The objective of this study is to detect aflatoxins in a large number of foods by a high efficient analytical system of combined enzyme linked immunosorbent assay (ELISA) for screening and LC/MS/MS for confirmation. The samples spiked individually with aflatoxin $B_1$ (0.5 and 1.0 ng/g) and total aflatoxins (10 ng/g) were analyzed by ELISA and LC/MS/ MS, and the recoveries for ELISA and LC/MS/MS were 71.8~119.2% and 70.8~135.3%, respectively. A total of 378 samples (grains, nuts, soybean and fermented soybean foods, pepper and fermented pepper foods) were purchased from the six major cities in Korea and analyzed by ELISA-LC/MS/MS system. Twenty two (5.8%; peanut: 11, pistachio: 2, walnut: 6, almond: 1, pepper powder: 1, pepper paste: 1) out of 378 samples were screened as aflatoxin B1 positive by ELISA, but, 4 (1.1%; peanut: 2, pistachio:1, pepper powder: 1) out of the 22 samples screened were confirmed as aflatoxins positive at levels of 1.02~52.79 ng/g by LC/MS/MS. ELISA-LC/MS/MS system provides a more rapid, accurate and cost-effective method for the detection of aflatoxins in large number of samples.

Keywords

References

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