Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
권호 표지

The Immunostimulatory Activity of The Water-Extract of Korean Mistletoe Fruit to Activate Murine Peritoneal Macrophages

한국산 겨우살이 열매 추출물의 마우스 복강 대식세포 면역활성화 효과

  • Lee, Jung-Lim (Department of Microbiology, College of medicine, Konyang University) ;
  • Jeon, Young-Ha (Department of Microbiology, College of medicine, Konyang University) ;
  • Yang, Hyo-Seon (Department of Microbiology, College of medicine, Konyang University) ;
  • Lee, Kyung-Bok (Department of Biochemistry, College of medicine, Konyang University) ;
  • Song, Kyung-Sik (Division of Applied Biology & Chemistry, Kyungpook National University) ;
  • Kang, Tae-Bong (Department of Biotechnology & Food Science, Handong University) ;
  • Kim, Jong-Bae (Department of Biotechnology & Food Science, Handong University) ;
  • Yoo, Yung-Choon (Department of Microbiology, College of medicine, Konyang University)
  • 이정림 (건양대학교 의과대학 미생물학교실) ;
  • 전영하 (건양대학교 의과대학 미생물학교실) ;
  • 양효선 (건양대학교 의과대학 미생물학교실) ;
  • 이경복 (건양대학교 의과대학 생화학교실) ;
  • 송경식 (경북대학교 응용생물화학부) ;
  • 강태봉 (한동대학교 생명식품공학부) ;
  • 김종배 (한동대학교 생명식품공학부) ;
  • 유영춘 (건양대학교 의과대학 미생물학교실)
  • Received : 2010.04.29
  • Accepted : 2010.05.13
  • Published : 2010.06.30
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Abstract

Mistletoe (Viscum album) is a common name for many species of semi-parasitic plants which grow on deciduous trees all over the world. In this study, the immunomodulatory activity of the water-extract of Korean mistletoe fruits (KMFWE), was investigated on murine peritoneal macrophages. The culture supernatants of KMF-WE-stimulated murine peritoneal macrophages showed the increased production of IFN-$\gamma$, IL-$1{\beta}$ and TNF-$\alpha$, in a dose-dependent manner. KMF-WE also induced chemokine production from murine peritoneal macrophages such as RANTES, MCP-1, MIP-$1{\alpha}$ and MIP-$1{\beta}$, as well as nitric oxide (NO) production, in a dose-dependent manner. The gel filtration fraction revealed F-1, which is the early-eluted and high molecular weight product, is the major fraction of KMF-WE to activate the murine peritoneal macrophage to induce cytokines, chemokines and NO. The nature of F-1 fraction needs to be examined in detail in further studies to define the regulatory mechanisms of cytokine or chemokine induction by KMF-WE on macrophages. These results suggest that KMF-WE possess a potent immunostimulant activity and can be a promising candidate available for development of immunomodulators.

Keywords

References

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