Journal of Korean Society of Forest Science (한국산림과학회지)

Korean Society of Forest Science (한국산림과학회)
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Skin Whitening and Skin Immune Activities of Different Parts of Acer mono and Acer okamotoanum

고로쇠 및 우산고로쇠나무의 부위별 미백 및 피부면역 활성

  • Jeong, Myoung-Hoon (College of Bioscience and Biotechnology, Kangwon National University) ;
  • Kim, Seung-Seop (College of Bioscience and Biotechnology, Kangwon National University) ;
  • Kim, Ji-Seon (College of Bioscience and Biotechnology, Kangwon National University) ;
  • Lee, Hak-Ju (Korea Forest Research Institute) ;
  • Chio, Geun-Pyo (Department of Food Processing & Bakery, Gangneung Provincial Univ.) ;
  • Lee, Hyeon-Yong (College of Bioscience and Biotechnology, Kangwon National University)
  • 정명훈 (강원대학교 BT특성화학부(대학)) ;
  • 김승섭 (강원대학교 BT특성화학부(대학)) ;
  • 김지선 (강원대학교 BT특성화학부(대학)) ;
  • 이학주 (국립산림과학원) ;
  • 최근표 (강원도립대학 식품가공제과제빵과) ;
  • 이현용 (강원대학교 BT특성화학부(대학))
  • Received : 2010.06.24
  • Accepted : 2010.07.08
  • Published : 2010.08.30
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Abstract

UV-protection skin whitening and immune activities several parts of Acer mono and Acer okamotoanum were investigated. The bark of both Acer mono and Acer okamotoanum had higher yields than other parts as 2.67% and 2.45%. The cytotoxicity of the extracts were lower than 21.64% against human skin cell(CCD-986sk) line in adding 1.0 mg/mL of the highest concentration. The bark extracts of Acer mono greatly reduced the expression of MMP-1 on UV-irradiated CCD-986sk cells down to as 30%. At 1.0 mg/mL of bark extration of Acer mono, $PGE_2$ expression was also significantly decreased. Generally, the bark extracts of Acer mono and Acer okamotoanum had higher activity than other parts, but, interestingly, wood extract of Acer okamotoanum showed strong inhibition effect on melanin production by Clone-M3 cells as 79.25%. From these results, we could conclude that the bark extract from Acer mono and Acer okamotoanum had skin-whitening activity as well immune enhancement activity.

고로쇠 및 우산고로쇠나무의 각 부위별 UV보호능에 의한 피부미백과 피부면역활성에 대해 연구하였다. 고로쇠 및 우산고로쇠나무의 수피 부위는 다른 부위의 2.67% 그리고 2.45%보다 높은 추출 수율을 나타내었다. 섬유아세포인 CCD-986sk에 대한 세포독성 결과 1.0 mg/mL의 농도에서 21.64%보다 낮은 세포독성을 보였다. 고로쇠나무 수피 추출물은 UV를 조사한 CCD-986sk 세포에서 30%이하의 MMP-1 발현을 억제하였다. 1.0 mg/mL의 고로쇠 수피 추출물의 경우 $PGE_2$ 발현이 상당히 감소되는 것을 확인할 수 있었다. 일반적으로 고로쇠 및 우산고로쇠나무의 수피 추출물이 다른 부위보다 높은 활성을 보이는데 반해 흥미롭게도 우산고로쇠나무의 목부 추출물은 Clone-M3세포에서 79.25%의 높은 멜라닌 생성저해 활성을 보였다. 이러한 결과로부터 고로쇠 및 우산고로쇠 수피 추출물이 목부 추출물에 비해 높은 향장활성을 보임을 확인할 수 있었고, 이들 추출물이 피부미백과 피부면역활성을 가짐으로서 앞으로 향장소재로서의 개발 가능성을 확인하였다.

Keywords

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