The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Diversity and Pathogenicity of Fusarium Species Associated with Head Blight of Job's Tears

율무 이삭마름 증상에서 분리한 Fusarium속 균의 다양성 및 병원성

  • Choi, Hyo-Won (Crop Protection Division, National Academy of Agricultural Science, RDA) ;
  • Hong, Sung-Kee (Crop Protection Division, National Academy of Agricultural Science, RDA) ;
  • Kim, Wan-Gyu (Agricultural Microbiology Team, National Academy of Agricultural Science, RDA) ;
  • Lee, Young-Kee (Crop Protection Division, National Academy of Agricultural Science, RDA)
  • 최효원 (농촌진흥청 국립농업과학원 작물보호과) ;
  • 홍성기 (농촌진흥청 국립농업과학원 작물보호과) ;
  • 김완규 (농촌진흥청 국립농업과학원 농업미생물팀) ;
  • 이영기 (농촌진흥청 국립농업과학원 작물보호과)
  • Received : 2011.11.16
  • Accepted : 2011.11.21
  • Published : 2011.12.01
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Abstract

Job's tears (Coix lacryma-jobi) is native to East Asia, and grains of the plant are used as health food and medicinal material. Head blight symptoms of the plant were frequently observed during disease surveys in Korea from 2006 to 2008. The symptoms were characterized as discoloration of husks, and subsequently inside of mature grains were shriveled or emptied. One hundred fifty nine isolates of Fusarium species were obtained from the disease symptoms of the plant collected from several locations in the country. Out of the isolates, the most frequently isolated Fusarium species were F. graminearum (34%), F. proliferatum (14.5%), F. verticillioides (10.1%), F. equiseti (6.9%), and F. fujikuroi (6.3%). Other Fusarium species isolated were F. subglutinans, F. semitectum, F. poae, and F. sporodochioides. Elongation factor 1 alpha gene sequences of the isolates were used for phylogenetic analysis. Analyses of the sequences revealed that the isolates were confirmed to be identical with each reference species of NCBI GenBank. Pathogenicity tests showed that F. graminearum, F. proliferatum and F. verticillioides were strongly virulent to grains of Job's tears. The present study is the first report of head blight of Job's tears caused by Fusarium species in Korea.

율무(Coix lacryma-jobi)는 동아시아에 자생하는 작물로, 종실은 주로 건강식품이나 의약용으로 사용되고 있다. 2006년부터 2008년까지 국내 율무 재배 지역에서 이삭마름 증상이 관찰되었다. 병징은 겉껍질이 변색되고, 내부의 속씨는 마르고 쭉정이가 되는 것이다. 159개의 Fusarium균을 분리하였고, 분리 빈도별로 F. graminearum(34%), F. proliferatum(14.5%), F. verticillioides(10.1%), F. equiseti(6.9%), F. fujikuroi(6.3%) 순으로 분리되었고, 그 밖에 F. subglutinans, F. semitectum, F. poae, F. sporodochioides가 분리되었다. 분리균주의 elongation factor 1 alpha 유전자의 염기서열 분석을 통한 계통분석을 수행한 결과, NCBI GenBank에 등록된 균주와 동일한 것을 확인하였다. 분리균주의 병원성 검정을 수행한 결과, F. graminearum, F. proliferatum, F. verticillioides가 병원성이 강하였다. 이 연구는 Fusarium균에 의해 발생한 율무 이삭마름 증상에 대한 첫 번째 보고이다.

Keywords

References

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