Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Screen of Functional Activity of Polysaccharide and Glycosaminoglycan from Sea Hare (Aplysia kurodai) by Cell Line

세포주를 이용한 군소 다당류와 Glycosaminoglycan의 기능성 검색

  • Hong, Yu-Mi (Dept. of Seafood Science and Technology/Institute of Marine Industry, Gyeongsang National University) ;
  • Park, Si-Hyang (Dept. of Seafood Science and Technology/Institute of Marine Industry, Gyeongsang National University) ;
  • Yoon, Bo-Yeong (Dept. of Seafood Science and Technology/Institute of Marine Industry, Gyeongsang National University) ;
  • Choi, Byeong-Dai (Dept. of Seafood Science and Technology/Institute of Marine Industry, Gyeongsang National University) ;
  • Choi, Yeung-Joon (Dept. of Seafood Science and Technology/Institute of Marine Industry, Gyeongsang National University)
  • 홍유미 (경상대학교 해양식품공학과/해양산업연구소) ;
  • 박시향 (경상대학교 해양식품공학과/해양산업연구소) ;
  • 윤보영 (경상대학교 해양식품공학과/해양산업연구소) ;
  • 최병대 (경상대학교 해양식품공학과/해양산업연구소) ;
  • 최영준 (경상대학교 해양식품공학과/해양산업연구소)
  • Received : 2010.09.30
  • Accepted : 2010.10.31
  • Published : 2011.01.31
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Abstract

In this study, we extracted a whole polysaccharide fraction from the sea hare, Aplysia kurodai, and screened its functional properties using cell lines. The functionalities of polysaccharide and glycosaminoglycan (GAG) were investigated with RAW 264.7 cell lines. The crude polysaccharides and GAG purified DEAE-Sepharose chromatography did not show the toxicity on RAW 264.7 cell line in the range of $10\sim200{\mu}g$/mL, whereas they increased the cell growth rate. The crude polysaccharides and purified GAG also increased the production of NO, interleukin-6 and tumor necrosis factor-$\alpha$ on RAW 264.7 cell. Particularly, the purified GAG inhibited the proliferation of stomach cancer cell line, AGS, up to 40% for 72 hr incubation, but not the intestinal epithelial IEC-6 cell lines.

군소에서 정제한 GAG는 대식세포주인 RAW 264.7에서 세포독성은 보이지 않았으며 NO 생성량, TNF-$\alpha$, IL-6 생성량을 증가시켜 면역조절 효과가 있음을 확인하였다. 그리고 $100{\mu}g$/mL 농도에서 배양 72시간 후 약 40%에 가까운 위암세포(AGS) 성장 억제 효과가 나타났고, 정상세포 IEC-6에는 독성을 보이지 않았다. 이 같은 결과는 면역조절을 위한 기능성식품 혹은 위암 치료제로서 군소의 당 추출물과 GAG의 활용 가능성을 제시한다.

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