The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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The effect of Gyungokgo-gamibang extract on hair growth and protein expression in mice

경옥고가미방 추출물이 생쥐의 양모 및 발모 관련 단백 발현에 미치는 영향

  • Do, Eun-Ju (Daegu Technopark Oriental Medicine Industry Support Center) ;
  • Hwang, Mee-Yul (The Research Center for Biomedical Resources of Oriental Medicine, Daegu Haany University) ;
  • Kim, Seung-Yeon (Daegu Technopark Oriental Medicine Industry Support Center) ;
  • Lee, Jin-Sang (Daegu Technopark Oriental Medicine Industry Support Center) ;
  • Yang, Dae-Seok (Yangnyeongsi People Co., Ltd.) ;
  • Yang, Chae-Ha (The Research Center for Biomedical Resources of Oriental Medicine, Daegu Haany University) ;
  • Kim, Mi-Ryeo (Daegu Technopark Oriental Medicine Industry Support Center)
  • 도은주 ((재)대구테크노파크 한방산업지원센터) ;
  • 황미열 (대구한의대학교 한방생명자원연구센터) ;
  • 김승연 ((재)대구테크노파크 한방산업지원센터) ;
  • 이진상 ((재)대구테크노파크 한방산업지원센터) ;
  • 양대석 ((주)약령시사람들) ;
  • 양재하 (대구한의대학교 한방생명자원연구센터) ;
  • 김미려 ((재)대구테크노파크 한방산업지원센터)
  • Received : 2011.11.06
  • Accepted : 2011.12.16
  • Published : 2011.12.30
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Abstract

Objective : Present study was carried out to investigate the effect of Gyungohkgo-gamibang extract on hair growth and protein expression in an alopecia model of C57BL/6 mice. Methods : Mice were divided into 3 experimental groups including normal (vehicle), Gyungohkgo-gamibang extract (YNS-10) and 5% minoxidil-treated group. The test materials were daily applied with 0.1 ml per mouse on shaved dorsal skin for 3 weeks. The hair growth was monitored by photograph at 5, 10, 15, 21 days after topical application. Then the changes of hair density and hair thickness in the hair-removed area were evaluated by phototrichogram using folliscope. Also the expression level of growth factors related to hair growth was measured by western blotting. Results : Application of minoxidil or YNS-10 stimulated the hair growth compared to vehicle treatment. Therefore hair density of minoxidil or YNS-10 application was increased about 200% and 210% more than in vehicle application on 14 day, respectively. And hair thickness of both minoxidil group and YNS-10 group was increased about 220% and 210 % more than in vehicle spreading on 14 day, respectively. Futhermore the protein expression of IGF-1 and VEGF were significantly up-regulated on 7 day in YNS-10 and minoxidil-spreaded group compared to vehicle-applied group. Conclusion : These data suggest that YNS-10 has potent stimulating activity on hair growth in C57BL/6 mice and potential usefulness as ingredients of hair tonic and hairrestore.

Keywords

References

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