The Journal of Internal Korean Medicine (대한한방내과학회지)

The Society of Internal Korean Medicine (대한한방내과학회)
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Protective Effects of Yinjinchunggan-tang (YJCGT) on Alcohol-induced Oxidative Stress

인진청간탕(茵蔯淸肝湯)의 알코올성 산화스트레스에 대한 보호효과 연구

  • Kim, Young-Tae (Dept. of Internal Medicine, College of Oriental Medicine, Kyung-Hee University) ;
  • Woo, Hong-Jung (Dept. of Internal Medicine, College of Oriental Medicine, Kyung-Hee University)
  • 김영태 (경희대학교 한의과대학 간계내과학교실) ;
  • 우홍정 (경희대학교 한의과대학 간계내과학교실)
  • Published : 2011.12.30
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Abstract

Objectives : Oxidative stress seems to play a major role in mechanisms by which ethanol causes liver injury. Previous studies have shown that treatment with Yinjinchunggan-tang (Yinchenqinggan-tang, YJCGT) has protective effects on alcoholic liver disease. The aim of this study was to investigate the protective effects of YJCGT on alcohol-induced oxidative stress. Materials and Methods : In vitro, we evaluated the inhibitory activities of YJCHT on DPPH(1,1-diphenyl-2-picryl-hydrazyl), xanthine oxidase, trypsin, and hyaluronidase. In a cell culture model, we measured cell viability and proliferation, and the activities of superoxide dismutase (SOD), and catalase (CAT) after YJCGT treatment in C34 and E47 cell lines, and HepG2 cells transfected with/ without cytochrome P450IIE1 (CYP2E1) gene. In vivo, we estimated serum level of hepatic biochemical markers, and alcohol concentration in the blood. Results : YJCGT showed significant free radical scavenging activity against DPPH and xanthine oxidase and decreased hyaluronidase activity effectively in vitro. YJCGT also increased cell viability, and proliferation in C34 and in E47 cell lines, and increased activities of superoxide dismutase, and catalase in C34 and in E47 cell lines. YJCGT reduced serum AST, LDH, and total cholesterol level in some of the results, and reduced blood alcohol concentration in vivo, as well. Conclusions : This study suggests that YJCGT has protective effects on oxidative stress by inhibiting alcohol-induced suppression of antioxidant enzyme activities.

Keywords

References

  1. Steinmetz KA, Potter JD. Vegetables, fruit and cancer. II. mechanisms. Cancer Causes Control 1991;2:427-42. https://doi.org/10.1007/BF00054304
  2. Freidovich I. Fundamental aspects of reactive oxygen species, or what's the matter with oxygen? Ann NAY Acad Sci 1999;893:13-5. https://doi.org/10.1111/j.1749-6632.1999.tb07814.x
  3. Wu G, Morris SM. Arginine metabolism : Nitric oxide and beyond. Biochem J 1998;336:1-6.
  4. Lee SO, Lee HJ, Yu MH, Im HG, Lee IS. Total polyphenol contents and antioxidant activities of methanol extracts from vegetables produced in Ulung island. Korean J Food Sci Technol 2005;37:233-40.
  5. Shin EM, Kim DH, Kwon YB, Kim YS. Antiinflammatory and immunomodulatory effects of the botanical product AMP-365TM. Korean J Pharmacogn 2006;37:212-6.
  6. Tuma DJ, Casey CA. Dangerous by-products of alcohol breakdown－focus on adducts. Alcohol Res Health 2003;27:285-90.
  7. Das SK, Vasudevan DM. Alcohol-induced oxidative stress. Life Science 2007;81(3):177-87. https://doi.org/10.1016/j.lfs.2007.05.005
  8. Castillo T, Koop DR, Kamimura S, Triadafilopoulos G, Tsukamoto H. Role of cytochrome P-450 2E1 in ethanol-carbon tetrachloride and iron-dependent microsomal lipid peroxidation. Hepatology 1992;16:992-6. https://doi.org/10.1002/hep.1840160423
  9. 張仲景. 仲景全書. 서울: 大星文化社; 1984, p. 225, 240, 249, 250, 408, 411.
  10. 金榮哲, 李長勳, 禹弘楨. 茵蔯淸肝湯의 안전성에 관한 연구. 경희한의대논문집 1997:20(1): 57-89.
  11. 金珍珠. 茵蔯淸肝湯이 MHV-2로 유발된 마우스의 손상간에 미치는 영향. 학위논문(석사). 경희대학교 대학원; 1996.
  12. 禹弘楨. 만성B형간염에 대한 茵蔯淸肝湯의 효과. 제2회 한중 학술대회 참가 논문집(간장병) 1995:18-53.
  13. 강우성. HCV 환자에서 茵蔯淸肝湯가미방 투여후 Anti-HCV역가의 변화관찰 15예. 전국한의학 학술대회 논문집 1998:85-6.
  14. 姜京台, 李長勳, 禹弘楨. 茵蔯淸肝湯가미방이 실험적 흰쥐의 간경변증에 미치는 영향. 경희한의대논문집 1997;20(2):133-50.
  15. 朴容禛, 金榮哲, 李長勳, 禹弘楨. 茵蔯淸肝湯가미방이 간세포의 증식능력에 미치는 영향. 대한한의학회지 1998;19(1):145-64.
  16. 洪尙勳, 李長勳, 禹弘楨. 茵蔯淸肝湯가미방이 간세포활성세포주기 및 apoptosis에 미치는 영향. 대한한의학회지 1998;19(2):337-72.
  17. 승현석, 김영철, 이장훈, 우홍정. 茵蔯淸肝湯이 간보호 및 섬유화 억제에 미치는 영향. 대한한방내과학회지 2003;24(1):21-32.
  18. 2009 국민건강통계. 국민건강영양조사 제4기 3차년도(2009). 보건복지부, 질병관리본부; 2010, p. 22-3.
  19. 김영철, 이장훈, 우홍정. 茵蔯淸肝湯이 횐쥐의 간장 비실질세포의 procollagen 합성억제에 미치는 효과에 관한 연구. 대한한방내과학회지 2003;24(4):817-25.
  20. 박상백, 김영철, 이장훈, 우홍정. 茵蔯淸肝湯이 DMN 유발 간섬유화와 단백질 발현에 미치는 영향. 대한한방내과학회지 2008;29(1):200-18.
  21. 박신명, 이장훈, 김영철, 우홍정. TAA로 유발된 간섬유화 동물모델에서 茵蔯淸肝湯의 효과. 대한한방내과학회지 2009;30(2):270-87.
  22. 이홍일, 김영철, 우홍정, 이장훈. 茵蔯淸肝湯이 간성상세포의 섬유화 억제에 미치는 영향. 대한한방내과학회지 2009;30(1):74-84.
  23. Cederbaum AI, Lu Y, Wu D. Role of oxidative stress in alcohol-induced liver injury. Arch Toxicol 2009;83:519-48. https://doi.org/10.1007/s00204-009-0432-0
  24. 지형준. 대한약전 및 대한약전외 한약규격주해. 서울: 한국메디칼인덱스사; 1998, p. 58-700.
  25. McCord JM, Fridovich I. Superoxide dismutase. An enzymic function for erythrocuprein(hemocuprein). J Biol Chem 1969;244:6049-55.
  26. Johansson LH, Borg LA. A spectrophotometric method for determination of catalase activity in small tissue samples. Anal Biochem 1988; 174(1):331-6. https://doi.org/10.1016/0003-2697(88)90554-4
  27. Blois MS. Antioxidant determinations by the use of a stable free radical. Nature 1958;181:1199-200. https://doi.org/10.1038/1811199a0
  28. Anson ML. The estimation of pepsin, trypsin, papain and cathepsin with hemoglobin. J Gen Physiol 1938;22:79-89. https://doi.org/10.1085/jgp.22.1.79
  29. Uchiyama T, Kamikawa H, Ogita ZI. Antiinflammatory effect of extract from Phellodendri cortex. J Med Pharm Soc Wkan Yaku 1989;6:158-64.
  30. Bergmeyer HU, Bernt E, Schmidt F, Stork H. Methods of Enzymatic Analysis(Bergmeyer, H.U., ed.). New York: Verlag Chemie, Weinheim, and Academic Press; 1974, p. 944.
  31. Kim YS, Noh YK, Lee GI, Kim YK, Lee KS, Min KR/ Inhibitory effects of herbal medicines on hyaluronidase activity. Kor J Pharmcogn 1995;26(3):265-72.
  32. Reitman S, Flankel S. A colorimetric method for the determination of serum glutamic oxaloacetic and glutamic pyruvic transaminase. Am J Clin Pthol 1992;40:2287-91.
  33. Shanmugasundaram P, Venkataraman S. Hepatoprotective and antioxidant effects of Hygrophila auriculata(K. Shum) Heine Acanthaceae root extract, J Ethnopharm 2006;104:124-8. https://doi.org/10.1016/j.jep.2005.08.058
  34. Wroblewskin F, LaDue JS. Lactic dehydrogenase activity in blood. Proc Soc Exp Biol Med 1955;90:210-3.
  35. Szasz G. A kinetic photometric method for serum $\gamma$-glutamyl transpeptidase. Clin Chem 1969;15:124-36.
  36. Haglund O, Luostarinen R, Wallin R, Wibell L, Saldeen T. The effects of fish oil on triglycerides, cholesterol, fibrinogen and malodialdehyde in humans supplemented with Vitamin E. J Nutr 1991;121:165-9.
  37. Sardesai VM, Manning JA. The determination of triglycerides in plasma and tissues. Clin Chem 1968;14:156-61.
  38. Cho MH, Shim SM, Lee SR, Mar W, Kim GH. Effect of Evodiae fructus extracts on gene expressions related with alcohol metabolism and antioxidation in ethanol-loaded mice. Food Chem Toxicol 2005;43(9):1365-71. https://doi.org/10.1016/j.fct.2005.03.010
  39. Lieber CS, Baraona E, Hernandez-Munoz R, Kubota S, Sato N, Kawano S, et al. Impaired oxygen utilization. A new mechanism for the hepatotoxicity of ethanol in sub-human primates. J Clin Invest 1989;83(5):1682-90. https://doi.org/10.1172/JCI114068
  40. Kessova I, Cederbaum AI. CYP2E1: biochemistry, toxicology, regulation and function in ethanolinduced liver injury. Curr Mol Med 2003;3:509-18. https://doi.org/10.2174/1566524033479609
  41. Morimoto M, Hagbjork AL, Wan YJ, Fu PC, Clot P, Albano E, et al. Modulation of experimental alcohol-induced liver disease by cytochrome P450 2E1 inhibitors. Hepatology 1995;21:1610-7.
  42. Bradford BU, Kona H, Isayama F, Kosyk O, Wheeler MD, Akiyama TE, et al. Cytochrome P450 CYP2E1, but not nicotinamide adenine dinucleotide phosphate oxidase is required for ethanol-induced oxidative DNA damage in rodent liver. Hepatology 2005;41:336-44. https://doi.org/10.1002/hep.20532
  43. Bradford BU, Kona H, Isayama F, Kosyk O, Wheeler MD, Akiyama TE, et al. Cytochrome P450 CYP2E1, but not nicotinamide adenine dinucleotide phosphate oxidase is required for ethanol-induced oxidative DNA damage in rodent liver. Hepatology 2005;41:336-44. https://doi.org/10.1002/hep.20532
  44. Korycka-Dahl M, Richardson T, Hicks C. Superoxide Dismutase Activity in Bovine Milk Serum. J Food Protection 1979;42:867-71.
  45. Baudrimont I, Ahouandjivo R, Creppy EE. Prevention of lipid peroxidation induced by ochratoxin A in vero cells in culture by several agents. Chem Biol Interact 1997;104:29-40. https://doi.org/10.1016/S0009-2797(97)03764-2
  46. Cassidy DA, Mcleod R, Clarke DJ. Regulation of hepatic xanthine oxidoreductase expression by treatment of rats with xenobiotic agents. Biochem Soc Trans 1997;25(4):563.
  47. 김은영, 백인희, 김정현, 김성란, 유미라. 항산화활성을 나타내는 약용식물 소재 탐색. 한국식품과학회지 2004;36(2):333-8.
  48. 문지숙, 김선재, 박윤미, 황인식, 김의형, 박정 욱, 등. 약용식물 추출물에 대한 항미생물 활성 검색과 폴리페놀 함량. 한국식품저장유통학회지 2004;11(2):207-13.
  49. McCord JM. Free radicals and inflammation: protection of synorial fluid by superoxide dismutase. Science 1974;185:529-31. https://doi.org/10.1126/science.185.4150.529
  50. 문태철, 정광원, 정규찬, 손건호, 김현표, 강삼식, 등. 천연물로부터 염증성 포스포리파제 A2 저해제 검색. 약학회지 1997;41(5):565-70.
  51. 박종훈, 최혁재, 정석희, 김남재, 김동현. 빈용 한약재의 진통 소염 활성. 생약학회지 2001;32(4):257-68.
  52. 최수임, 이윤미, 허태련. 생약재 추출물의 hyaluronidase 저해 및 라디칼 소거 활성 검색. 한국생물공학회지 2003;18(4):282-8.
  53. Inkeles S, Eisenberg D. Hyperlipidemia and coronary atherosclerosis. Medicine(Baltimore) 1981;60:110-23.
  54. Elko EE, Wooles WR, Diluzio NR. Alterations and mobilization of lipids in acute ethanol-treated rats. Amer J Physiol 1961;201:923-6.