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Antioxidant Activities of Various Solvent Extracts from Canola Meal

추출용매별 canola meal의 항산화 활성

  • Jun, Hyun-Il (Department of Food Science and Technology, Chonbuk National University) ;
  • Wiesenborn, Dennis P. (Department of Agricultural and Biosystems Engineering, North Dakota State University) ;
  • Kim, Young-Soo (Department of Food Science and Technology, Chonbuk National University)
  • Received : 2010.09.16
  • Accepted : 2011.01.07
  • Published : 2011.02.28

Abstract

This study was performed to investigate antioxidant activity of various solvent extracts (methanol, ethanol, acetone and water) from canola meal. As the concentration of all extracts increased, antioxidant activities increased gradually. Among solvent extracts from canola meal, methanol extract showed the highest antioxidant activities with $EC_{50}$ values of 0.9, 3.5, 1.3, and 8.5 mg/mL for DPPH radical scavenging ability, ABTS radical scavenging ability, reducing power, and chelating ability, respectively, and it had the highest total phenolics and total flavonoids contents. Furthermore, methanol extract was fractionated into three fractions (MF 1, MF 2, and MF 3) using Sephadex LH-20 column. The highest antioxidant activity was found on MF 2 for DPPH radical scavenging ability, on MF 3 for ABTS radical scavenging ability and reducing power, and on MF 1 for chelating ability at the same concentration of 1.5 mg/mL. The results indicated that each fraction containing various antioxidant materials seemed to have different influence at each antioxidant activity.

본 연구에서는 용매별(methanol, ethanol, acetone 및 water)로 추출한 canola meal의 항산화 활성을 조사하였다. 모든 canola meal 용매 추출물들은 첨가 농도가 증가할수록 항산화 활성이 점진적으로 증가하였다. 추출물 중에서 methanol 추출물이 가장 낮은 $EC_{50}$ (DPPH radical scavenging ability; 0.9 mg/mL, ABTS radical scavenging ability; 3.5 mg/mL, reducing power; 1.3 mg/mL and chelating ability; 8.5 mg/mL)과 가장 많은 항산화 물질 (total phenolics; $63.3\;{\mu}g$/mg, total flavonoids; $21.3\;{\mu}g$/mg)을 함유하는 것으로 나타났다. 보다 정확한 canola meal의 항산화 활성을 조사하기 위하여 항산화 활성이 높은 methanol 추출물을 sephadex LH-20 column을 이용하여 순차적으로 MF 1, MF 2 및 MF 3의 3개의 분획물을 얻었으며, 동일한 농도(1.5 mg/mL)에서 측정한 항산화 활성에서 DPPH radical scavenging ability는 MF 2(75.56%)에서, ABTS radical scavenging ability와 reducing power는 MF 3(31.17% and 1.60)에서, chelating ability는 MF 1(77.72%)에서 가장 높게 나타났다.

Keywords

References

  1. Cevallos-Casals BA, Cisneros-Zevallos LCZ (2010) Impact of germination on phenolic content and antioxidant activity of 13 edible seed species. Food Chem, 119, 1485-1490 https://doi.org/10.1016/j.foodchem.2009.09.030
  2. Siddhuraju P (2007) Antioxidant activity of polyphenolic compounds extracted from defatted raw and dry heated Tamarindus indica seed coat. Lebensmittel Wissenscaft und Technologie, 40, 982-990 https://doi.org/10.1016/j.lwt.2006.07.010
  3. Siddhuraju P, Manian S (2007) The antioxidant activity and free radical-scavenging capacity of dietary phenolic extracts from horse gram (Macrotyloma uniflorum (Lam.) Verdc.) seeds. Food Chem, 105, 950-958 https://doi.org/10.1016/j.foodchem.2007.04.040
  4. Chung TY (1989) Nutritive values of canola meal and full-fat canola seed. Kor J Anim Feed, 13, 271-284
  5. Roh KH, Park JS (2007) Biodiesel; oil-crops and biotechnology. J Korean Soc Appl Biol Chem, 50, 137-146
  6. Khattab RY, Arntfield SD (2009) Functional properties of raw and processed canola meal. Lebensmittel Wissenscaft und Technologie, 42, 1119-1124 https://doi.org/10.1016/j.lwt.2009.02.009
  7. Naczk M, Amarowicz R, Sullivan A, Shahidi F (1998) Current research developments on polyphenolics of rapeseed/canola: a review. Food Chem, 62, 489-502 https://doi.org/10.1016/S0308-8146(97)00198-2
  8. Thiyam U, Kuhlmann A, StOckmann H, Schwarz K (2004) Prospects of fapeseed oil by-products with respect to antioxidative potential. C R Chimie, 7, 611-616 https://doi.org/10.1016/j.crci.2004.02.011
  9. Shahidi F, Wanasundara U (1995) Effect of natural antioxidant on the stability of canol oil. Developments in Food Sci, 37, 469-479 https://doi.org/10.1016/S0167-4501(06)80173-1
  10. Brettonnet A, Hewavitarana A, Dejong S, Lanari MC (2010) Phenolic acids composition and antioxidant activity of canola extracts in cooked beef, chicken and pork. Food Chem, 927-933
  11. Dewanto V, Wu X, Adonm KK, Liu, RH (2002) Thermal processing enhances the nutritional value of tomatoes by increasing total antioxidant activity. J Agric Food Chem, 50, 3010-3014 https://doi.org/10.1021/jf0115589
  12. Jia Z, Tang M, Wu J (1999) The determination of flavonoid contents in mulberry and they scavenging effects on superoxide radicals. Food Chem, 64, 555-559 https://doi.org/10.1016/S0308-8146(98)00102-2
  13. Williams WB, Cuvelier ME, Berset, C (1995) Use of a free radical method to evaluate antioxidant activity. Lebensmittel Wissenscaft und Technologie, 28, 25-30 https://doi.org/10.1016/S0023-6438(95)80008-5
  14. Arts MJTJ, Haenen GRMM, Voss HP, Bast A (2004) Antioxidant capacity of reaction products limits the applicability of the trolox equivalent antioxidant capacity (TEAC) assay. Food Chem Toxicol, 42, 45-49 https://doi.org/10.1016/j.fct.2003.08.004
  15. Oyaizu M (1986) Studies on products of browning reaction-antioxidant activities of products of browning reaction prepared from glucoamin. Japanese J Nutr, 44, 307-315 https://doi.org/10.5264/eiyogakuzashi.44.307
  16. Decker EA, Welch B (1990) Role of ferritin as a lipid oxidation catalyst in muscle food. J Agric Food Chem, 38, 674-677 https://doi.org/10.1021/jf00093a019
  17. Naczk M, Pegg RB, Zadernowski R, Shahidi F (2005) Radical scavenging activity of canola hull phenolics. J Am Oil Chem Soc, 82, 255-260 https://doi.org/10.1007/s11746-005-1064-7
  18. Hass-Roudsari M, Chang PR, Pegg RB, Tyler RT (2009) Antioxidant capacity of bioactives extracted from canola meal by subcritical water, ethanolic and hot water extraction. Food Chem, 114, 717-726 https://doi.org/10.1016/j.foodchem.2008.09.097
  19. Amarowicz R, RAAB B, Shahidi F (2003) Antioxidant activity of phenolic fractions of rapeseed. J Food Lipids, 10, 51-62 https://doi.org/10.1111/j.1745-4522.2003.tb00005.x
  20. Amarowicz R, Naczk M, Shahidi F (2000) Antioxidant activity of various fractions of non-tannin phenolics of cannola hulls. J Agric Food Chem, 48, 2755-2759 https://doi.org/10.1021/jf9911601
  21. Wanasundara UN, Amarowicz R. Shahidi F (1996) Partial characterization of natural antioxidants in canola meal. Food Res International, 28, 525-530

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