Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Enhancement of Whitening Effects of Lithospermum erythrorhizon Extracts by Ultra High Pressure

지치 초고압 추출물의 미백활성 증진

  • Kim, Ji-Seon (Medical & Bio-Material Research Center and College of Bioscience and Biotechnology, Kangwon National University) ;
  • Jeong, Myoung-Hoon (Medical & Bio-Material Research Center and College of Bioscience and Biotechnology, Kangwon National University) ;
  • Choi, Woon-Yong (College of Bioscience and Biotechnology, Kangwon National University) ;
  • Seo, Yong-Chang (Medical & Bio-Material Research Center and College of Bioscience and Biotechnology, Kangwon National University) ;
  • Ma, Choong-Je (College of Bioscience and Biotechnology, Kangwon National University) ;
  • Ahn, Ju-Hee (College of Bioscience and Biotechnology, Kangwon National University) ;
  • Kim, Nam-Seong (Department of Biology, Chonnam National University) ;
  • Hwang, Baik (Department of Biology, Chonnam National University) ;
  • Cho, Jeong-Sub (DooSan EcoBizNet) ;
  • Lee, Hyeon-Yong (Medical & Bio-Material Research Center and College of Bioscience and Biotechnology, Kangwon National University)
  • 김지선 (강원대학교 의료.바이오신소재융복합연구사업단) ;
  • 정명훈 (강원대학교 의료.바이오신소재융복합연구사업단) ;
  • 최운용 (강원대학교 바이오산업공학부) ;
  • 서용창 (강원대학교 의료.바이오신소재융복합연구사업단) ;
  • 마충제 (강원대학교 바이오산업공학부) ;
  • 안주희 (강원대학교 바이오산업공학부) ;
  • 김남성 (전남대학교 생물학과) ;
  • 황백 (전남대학교 생물학과) ;
  • 조정섭 (두산에코비즈넷) ;
  • 이현용 (강원대학교 의료.바이오신소재융복합연구사업단)
  • Received : 2010.10.02
  • Accepted : 2011.04.13
  • Published : 2011.04.30
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Abstract

In this study, whitening activity of Lithospermum erythrorhizon extracts were investigated according to several extraction processes: water extraction at $100^{\circ}C$ (WE100) and $60^{\circ}C$ (WE60), 70% ethyl alcohol extraction (EE) and ultra high pressure extraction (HPE) at 500 MPa for 30 minutes at $60^{\circ}C$. The extracts from ultra high pressure extraction showed the highest tyrosinase inhibition and melanogenesis inhibition activities as 52% and 79.5%, respectively, in adding $1mg/m{\ell}$ than others extraction processes. HPE extracts also showed the strong reducing power as 3.19 that absorbance at 450 mm. The contents of polyphenol in WE100, we measured as $10.1{\mu}g/m{\ell}$ in adding $1mg/m{\ell}$. Extracts have a high total flavonoid contents by HPE as $4.1{\mu}g/m{\ell}$ at $1mg/m{\ell}$. We can conclude that better whitening activity of extracts from high pressure extraction was due to high antioxidant activities which could be extracted by higher polyphenol and flavonoid contents in HPE than others.

Keywords

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