The Journal of Internal Korean Medicine (대한한방내과학회지)

The Society of Internal Korean Medicine (대한한방내과학회)
권호 표지

Effect of Yagwan-cheunghyeoltang on Obesity in Rats Induced by High Fat Diet

야관청혈탕이 고지방식이로 유도된 흰쥐의 비만 억제에 미치는 영향

  • Park, Sang-Woo (Dept. of Internal Medicine, College of Oriental Medicine, Dae-Jeon University) ;
  • Cho, Chung-Sik (Dept. of Internal Medicine, College of Oriental Medicine, Dae-Jeon University) ;
  • Kim, Chul-Jung (Dept. of Internal Medicine, College of Oriental Medicine, Dae-Jeon University)
  • 박상우 (대전대학교 한의과대학 신계내과학교실) ;
  • 조충식 (대전대학교 한의과대학 신계내과학교실) ;
  • 김철중 (대전대학교 한의과대학 신계내과학교실)
  • Published : 2011.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

Background : Obesity, the syndrome caused by a high fat diet, is a disease. At the same time, obesity causes diabetes mellitus, hyperlipidemia, and cardiovascular disease. Recently, its prevalence rate is increasing. Yagwan-cheunghyeoltang (YCT) used in this experiment is the prescription of Yagwanmoon added to Cheunghyeol-tang which is reported to be very effective in weight loss controlling and serum cholesterol. It is also reported that Yagwanmoon has significant antioxidant effects and YCT has a significant effect on blood glucose control. Objectives : This study was conducted to experimentally evaluate the effects of YCT on obesity in rats induced by high fat diet. Methods : The experiment was conducted with 4-week-old male rat s divided into 5 groups. They were a normal diet group, a high fat diet group, a positive drug control group, a 1% YCT group, and a YCT 3% group, and were tested for eight weeks. After four weeks of inducing obesity by a high fat diet, rats were allowed to lose weight by following the normal diet group, approximately 30% compared with 10 rats in each group were determined as still obese. Changes in body weight and organ weight and serum cholesterol, triglyceride, glucose-density, low density lipoprotein cholesterol, antioxidant activity were checked. Results : In the experimental groups, we observed weight loss and visceral fat reduction, improvement of liver function, reduction of serum glucose, activation of HMG-CoA reductase inhibitor, reduction of concentrations of leptin and it showed a significant effect on antioxidants and lipid peroxidation. Conclusions : YCT has significant effects on the regulation of hyperlipidemia and lipid peroxidation associated with obesity and has significant effects on, antioxidants and lipid peroxidation, too. Additional clinical studies are needed.

Keywords

References

  1. 서울대학교 의과대학편. 내분비학. 전정판. 서울: 서울대학교출판부; 2006, p. 425-45, 491-5.
  2. 민헌기. 임상내분비학. 서울: 고려의학; 1990, p. 476-7, 479, 482-6.
  3. 한방재활의학과학회. 한방재활의학과학. 서울: 군자출판사; 2003, p. 349-62.
  4. 대한비만학회. 임상비만학. 서울: 고려의학; 2001, p. 19-20, 27-9, 31-56, 75-84, 241, 303-11.
  5. Kasper DL, Fauci AS, Braunwald E, Hauser SL, Longo DL, Jameson JL, et al. 해리슨 내과학. 17th edition. 서울: MIP; 2010, p. 559-70. 1825-30. 2905-21.
  6. 국민건강영양조사. URL : http://knhanes.cdc.go.kr/.
  7. 통계청. 2009년 사망원인 통계결과. URL : http: //www.nso.go.kr.
  8. 양은주, 조영숙, 최명숙, 우명남, 김명주, 손미예,등. 보리순이 고지방을 급여한 마우스의 지질함량과 간조직의 지질대사 관련 효소활성에 미치는 영향. 한국영양학회지 2009;42(1):14-22.
  9. 강금지, 임숙자, 정종길, 한혜경, 최성숙, 김명화, 등. 고지방식이를 섭취시킨 흰쥐에서 동과가 체중 중성지방 leptin과 지방세포의 크기에 미치는 영향. 한국영양학회지 2003;36(5):446-51.
  10. 김유희, 유재욱, 이유진, 김경범, 조대현, 황진영. Phaseolus vulgaris, Garcinia cambogia(HCA)가 함유된 다이어트 식이 조성물의 체지방 개선 및 체중 변화에 미치는 영향. 한국식품영양과학회지 2004;33(3):518-22.
  11. 김만호, 조충식, 김철중. 청혈탕과 가미청혈탕이 비만에 미치는 영향. 대한한방내과학회지 2006;27(2):305-15.
  12. 김창민, 신민교, 안덕균, 이정순 등 번역. 中藥大辭典. 서울: 도서출판 정담; 1997, p. 2771.
  13. 이동성, 정길생, 안인파, 리빈, 변에리사, 김윤철. 산화적 스트레스에 대한 췌장 베타 세포보호활성 식물추출물 탐색. Kor J Pharmacogn 2008;39(4):335-40.
  14. 김진미, 조충식, 김철중. RIN-m5F 세포에서 야관청혈탕이 인슐린 분비에 미치는 영향. 대한한의학회지 2010;31(4):20-7.
  15. Folch J, Less M, Stanely GHS. A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 1957;226:497-502.
  16. Zlatkis A, Zak B. Study of a new cholesterol reagent. Anal Biochem 1969;29:143-6. https://doi.org/10.1016/0003-2697(69)90017-7
  17. Biggs HG, Erikson MJ, Wells RM. A manual colorimetric assay of triglycerides in serum. Clin Chem 1975;21:437-41.
  18. Kleinsek DA, Dugan RE, Baker TA. 2 3- hydroxy-3-methylglu taryl-CoA reductase from rat liver. Methods enzymol 1981;71:462-79.
  19. Bansal Sk, Love J, Gurtoo HL. High pressure liquid chromatogr aphic separation of multiple of cytochrome P-450. Biochem Biophys Res Commun 1983;117:268-74. https://doi.org/10.1016/0006-291X(83)91570-X
  20. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem 1951;193-265.
  21. Buege JA, SD Aust. Microsomal lipid peroxidation. In: Fleischer S, Packer L, eds. Methods in enzymology. 52th ed. London: Academic press; 1978, p. 302-9.
  22. Aebi H. Catalase in vitro. Methods Enzymol 1984;105:121-6.
  23. Lee DW, Yu BP. Modulation of free radicals and superoxide dismutase by age and dietary restriction. Aging (Milano) 1990;2(4):357-62.
  24. Koletzko B, Girardet JP, Klish W, Tobacco O. Obesity in children and adolescents worldwide. current views and future directions. J Pediatr Gastroenterol Nutr 2002;35:205-12. https://doi.org/10.1097/00005176-200208000-00019
  25. 두호경. 동의신계학. 서울: 성보사; 2003, p. 1265-81.
  26. Jena BS, Jayaprakasha GK, Singh RP, Sakariah KK. Chemistry and biochemistry of (-)-hydroxycitric acid from Garcinia. J Agric Food Chem 2002;50:10-22. https://doi.org/10.1021/jf010753k
  27. Sullivan AC, Singh M, Srere PA, Glusker JP. Reactivity and inhibit or potential of hydroxycitrate isomers with citrate synthase, citrate lyase, and ATP citrate lyase. J Biol Chem 1977;252:7583-90.
  28. Sullivan AC, Triscari J, Spiegel HE. Metabolic regulation as a contr ol for lipid disorders. II. Influence of (-)-hydroxycitrate on genetic ally and experimentally induced hypertriglyceridemia in the rat. Am J Clin Nutr 1977;30:777-84.
  29. Greenwood MR, Cleary MP, Gruen R, Blase D, Stern JS, Triscari J, et al. Effect of (-)-hydroxycitrate on development of obesity in the Zucker obese rat. Am J Physiol 1981;240:E72-8.
  30. 이정선, 손흥수, 맹영선, 장유경, 주진순. 메밀급여가 Streptozotocin 유발 당뇨쥐의 장기무게 및 당질과 지질대사에 미치는 영향. 한국영양학회지 1994;27(8):819-27.
  31. 정은영. 조릿대 잎 추출물이 비만 유발 C57BL/6J mice의 대사증후군 개선 효과에 관한 연구. 이학석사 학위논문(석사). 전남대학교대학원 ; 2006.
  32. 이귀녕, 권오헌. 임상병리파일. 서울: 의학문화사; 2003, p. 155-60, 334-7, 345-7, 399-404.
  33. 이우주, 홍사석, 조규철, 김원준, 홍기환, 유경자, 차영남, 김경환, 배영숙, 안영수. 이우주의 약리학강의. 3판. 서울: 의학문화사; 1993, p. 411-6.
  34. Anderson JW, Jones AE, Riddell-Mason S. Ten different dietary fib ers have significantly different effects on serum and liver lipids of cholesterol-fed rats. J Nutr 1994;124:78-83.
  35. Prins JB. Adipose tissue as an endocrine organ. Best Pract Res Clin Endocrinol Metab 2002;16:639-51. https://doi.org/10.1053/beem.2002.0222
  36. Caro JF, Sinha MK, Kolaczynski JW, Zhang PL, Considine RV. Leptin: the tale of an besity gene. Diabetes 1996;45:1455-62.
  37. Havel PJ. Role of adipose tissue in bodyweight regulation: mechanism regulating leptin production and energy nalance. proc Nutr Soc 2000;59(3):359-71. https://doi.org/10.1017/S0029665100000410
  38. Sokol RJ. Antioxidant defenses in metal-induced liver damage. Semi Liver Dis 1996;16(1):39-46. https://doi.org/10.1055/s-2007-1007217
  39. Andreazza AC. Kauer-Santanna M. Frey BN. Bond DJ. Kapczinski F. young LT, et al. Oxidative stress markers in bipolar disorder: a meta-analysis. J affect disord. 2008;111:135-44. https://doi.org/10.1016/j.jad.2008.04.013
  40. Ohkawa H, Ohish N, Yagi K. Assay for lipid peroxidation in animal tiss ues by thiobarbituric acid reaction. Anal Biochem 1979;95:351-7. https://doi.org/10.1016/0003-2697(79)90738-3
  41. Van Gaal LF, Zhang A, Steijaret MM, De Leeuw IH. Human obesity : from lipid abnormality to lipid oxidation. Int J Obes 1995;19(Suppl 3):S21-6.