Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Mechanism of Inhibition of HepG2 Cell Proliferation by a Glycoprotein from Hizikia fusiformis

톳(Hizikia fusiformis) 당단백질에 의한 HepG2 세포 증식 억제기전

  • Ryu, Jina (Department of Food and Life Science, Pukyong National University) ;
  • Hwang, Hye-Jung (Department of Food and Life Science, Pukyong National University) ;
  • Kim, In-Hye (Department of Food and Life Science, Pukyong National University) ;
  • Nam, Taek-Jeong (Department of Food and Life Science, Pukyong National University)
  • 류진아 (부경대학교 식품생명과학과) ;
  • 황혜정 (부경대학교 식품생명과학과) ;
  • 김인혜 (부경대학교 식품생명과학과) ;
  • 남택정 (부경대학교 식품생명과학과)
  • Received : 2012.07.18
  • Accepted : 2012.10.09
  • Published : 2012.12.31
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Abstract

Hizikia fusiformis, a brown alga that is widely consumed in Korea, Japan, and China, possesses a number of potentially beneficial compounds, including antioxidants and anticoagulants. However, the molecular mechanisms of H. fusiformis in hepatoma cells have not been elucidated. This study investigated the antiproliferative effect and mechanism of action of a glycoprotein from H. fusiformis (HFGP) in HepG2 human hepatoma cells. In an MTS assay, 25 ${\mu}g/mL$ HFGP inhibited the proliferation of HepG2 cells by $52.36{\pm}2.37%$. HFGP caused the dose-dependent growth inhibition of HepG2 cells by inducing apoptosis and a sub-G1 phase arrest. The antiproliferative activity of HFGP was confirmed based on the expression of several apoptosis-related proteins, which was assessed by Western blot analysis. The expressions of Fas, Fas-associated death domain protein, Bax, and Bad was significantly up-regulated in HFGP-treated cells, and HFGP induced the translocation of Bax to mitochondria and the release of cytochrome c into the cytosol. Therefore, HFGP might be useful in the treatment of liver cancer.

Keywords

References

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