Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Diagnosis Case of Viral Hemorrhagic Septicemia (VHS) in Adult Olive Flounder Paralichthys olivaceus

넙치(Paralichthys olivaceus) 성어의 viral hemorrhagic septicemia (VHS) 진단사례

  • Kim, In-Woo (Aquatic Life Disease Control Division, National Fisheries Research & Development Institute) ;
  • Cho, Mi Young (Aquatic Life Disease Control Division, National Fisheries Research & Development Institute) ;
  • Lee, Han-Na (Aquatic Life Disease Control Division, National Fisheries Research & Development Institute) ;
  • Han, Hyun Ja (Aquatic Life Disease Control Division, National Fisheries Research & Development Institute) ;
  • Oh, Yun Kyeong (Aquatic Life Disease Control Division, National Fisheries Research & Development Institute) ;
  • Lee, Soon Jeong (Aquatic Life Disease Control Division, National Fisheries Research & Development Institute) ;
  • Jee, Bo Young (Aquatic Life Disease Control Division, National Fisheries Research & Development Institute) ;
  • Myeong, Jeong-In (Southwest Sea Fisheries Research Institute, National Fisheries Research & Development Institute) ;
  • Won, Kyoung-Mi (Southwest Sea Fisheries Research Institute, National Fisheries Research & Development Institute)
  • 김진우 (국립수산과학원 수산생물방역과) ;
  • 조미영 (국립수산과학원 수산생물방역과) ;
  • 이한나 (국립수산과학원 수산생물방역과) ;
  • 한현자 (국립수산과학원 수산생물방역과) ;
  • 오윤경 (국립수산과학원 수산생물방역과) ;
  • 이순정 (국립수산과학원 수산생물방역과) ;
  • 지보영 (국립수산과학원 수산생물방역과) ;
  • 명정인 (국립수산과학원 남서해수산연구소) ;
  • 원경미 (국립수산과학원 남서해수산연구소)
  • Received : 2012.09.13
  • Accepted : 2012.11.27
  • Published : 2012.12.31
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Abstract

We examined the cause of a disease outbreak in adult olive flounder Paralichthys olivaceus, which occurred at a Korean aquaculture farm in Korea in 2011. The principal signs included an expanded abdomen and congested liver, with persistent mortality (a little over two months). At the beginning of the outbreak, farm administrators misjudged the disease as bacterial in origin, because of the aforementioned signs, persistent mortality, and the detection of bacterial species, including Vibrio spp. and Streptococcus spp. Moreover, the detection of viral hemorrhagic septicemia virus (VHSV) by reverse trasnscription-PCR analysis was complicated by use of the VHS-VN primer set, which has been in general use recently, because it produced weak bands in some samples. Therefore, we recommend the use of at least two different primer sets in the diagnosis of VHSV. Our histopathological findings indicate that necrotizing myocarditis could be considered a pathogenic sign of VHSV infection.

Keywords

References

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