Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
권호 표지
DOI QR코드

DOI QR Code

Optimization of Medium Composition for the Mycelial Growth of Sparassis crispa

꽃송이버섯의 균사 생장을 위한 배지 조건 최적화

  • Kim, Jin-Woo (Gyeongsangbuk-Do Forest and Environment Research Institute) ;
  • Cheon, Woo-Jae (Gyeongsangbuk-Do Forest and Environment Research Institute) ;
  • Chai, Kyung-Hee (Gyeongsangbuk-Do Forest and Environment Research Institute) ;
  • Kim, Dong-Gwan (Institute of Biotechnology, Vitrosys Incoporation) ;
  • Son, Sung-Ho (Institute of Biotechnology, Vitrosys Incoporation) ;
  • Kim, Jong-Guk (School of Life Science and Biotechnology, Kyungpook National University) ;
  • Lim, Hee-Jae (Gyeongsangbuk-Do Forest and Environment Research Institute)
  • 김진우 (경상북도산림환경연구원) ;
  • 천우재 (경상북도산림환경연구원) ;
  • 채경희 (경상북도산림환경연구원) ;
  • 김동관 ((주) 비트로시스 생명공학연구소) ;
  • 손성호 ((주) 비트로시스 생명공학연구소) ;
  • 김종국 (경북대학교 생명공학부) ;
  • 임희재 (경상북도산림환경연구원)
  • Received : 2011.12.05
  • Accepted : 2011.12.29
  • Published : 2012.02.28
Download PDF
⟨ Previous Next ⟩

Abstract

The characteristics of mycelium growth of Sparassis crispa KGFS08 and KFRI746 in liquid culture were investigated. The optimum growth of the mycelium of S. crispa was observed in the KTM medium. The best carbon source was starch. In terms of nitrogen sources, tryptone affected mycelial growth in the liquid culture. The optimal culture conditions were pH 4.0-5.0 in STK medium [3% (w/v) starch, 0.3% (w/v) tryptone, 0.1% (w/v) $KH_2PO_4$, and 0.1% (w/v) folic acid].

본 연구에서는 균사의 액체 배양 특성 조사를 위하여 꽃송이버섯 균주 KGFS08과 KFRI746를 선발하였다. 균사 생장을 위한 우수 배지는 KTM 배지로 조사되었으며, 탄소원과 질소원은 각각 3% (w/v) starch와 0.3% (w/v) tryptone, 비타민은 folic acid로 나타났다. 따라서 액체 배양 최적 조건은 STK 배지[3% (w/v) starch, 0.3% (w/v) tryptone, 0.1% (w/v) $KH_2PO_4$, 0.1% (w/v) folic acid], pH는 4.0~5.0으로 확인되었다.

Keywords

References

  1. Akihiro, K., K. Fumihisa, M. Godliving, and N. Yoshitoshi. 2006. Development of optimal culture method of Sparassis crispa mycelia and a new extraction method of antineoplastic constituent. Biochem. Eng. J. 30, 109-113. https://doi.org/10.1016/j.bej.2006.02.004
  2. Chang, H. Y. and S. O. Choi. 2004. Characteristics of mycelial culture of Sparassis crispa. J. Mushroom Sci. Production. 6, 163-167. https://doi.org/10.1515/IJMSP.2004.6.4.163
  3. Cheong, J. C., J. S. Park, I. P. Hong, S. J. Seok, C. S. Jhune, and C. J. Lee. 2008. Cultural characteristics of cauliflower mushroom, Sparassis crispa. Korean J. Mycol. 36, 16-21. https://doi.org/10.4489/KJM.2008.36.1.016
  4. Harada, T., N. N. Miura, Y. Adachi, M. Nakajima, T. Yadomae, and N. Ohno. 2002. IFN-${\gamma}$ induction by SCG, 1,3 ${\beta}$-D-glucan from Sparassis crispa, DBA/2 mice in vitro. J. Interferon Cytokine Res. 22, 1227-1239. https://doi.org/10.1089/10799900260475759
  5. Hiroshi, Y. 2005. Nutritional requirement for the vegetative growth of Sparassis crispa Wulf.: Fr. Kanto Gakuin University, Soc. Humanity Environ. Bull. 7, 69-78.
  6. Kirk, P. M., P. F. Cannon, J. C. David, and J. A. Stalpers. 2001. Ainsworth & Bisby's Dictionary of the Fungi. Ninth edition. CABI Bioscience.
  7. Oh, D. S. 2003. Studies on the optimal cultural media and conditions for mycelial growth of Sparassis crispa(Wulf.) Fr. Graduate School of Chonnam National University.
  8. Oh, D. S., J. M. Park, H. Park, K. H. Ka, and W. J. Chun. 2009. Site characteristics and vegetation structure of the habitat of cauliflower mushroom (Sparassis crispa). Korean J. Mycol. 37, 33-40. https://doi.org/10.4489/KJM.2009.37.1.033
  9. Ohno, N., N. N. Miura, M. Nakajima, and T. Yadomea. 2000. Antitumor 1,3-${\beta}$-glucan from cultured fruit body of Sparassis crispa. Biol. Pharm. Bull. 23, 866-872. https://doi.org/10.1248/bpb.23.866
  10. Park, H., B. H. Lee, K. H. Ka, W. C. Bak, D. S. Oh, J. M. Park, and W. J. Chun. 2006. Cultivation of cauliflower mushroom (Sparassis crispa) by use of steam-treated coniferous sawdusts. Mokchae Konghak 34, 84-89.
  11. Ryu, S. R., K. H. Ka, H. Park, W. C. Bak, and B. H. Lee. 2009. Cultivation characteristics of Sparassis crispa strains using sawdust medium of Larix kaempferi. Korean J. Mycol. 37, 49-54. https://doi.org/10.4489/KJM.2009.37.1.049
  12. Shim, J. O., S. G. Son, S. O. Yoon, Y. S. Lee, T. S. Lee, S. S. Lee, K. D. Lee, and M. W. Lee. 1998. The optimal factors for the mycelial growth of Sparassis crispa. Korean J. Mycol. 26, 39-46.
  13. Yamamoto, K., Y. Nishikawa, T. Kimura, M. Dombo, N. Matsuura, and A. Sugitachi. 2007. Antitumor activities of low molecular weight fraction derived from the cultured fruit body of Sparassis crispa in tumor-bearing mice. J. Jap. Soc. Food Sci. Technol. 54, 419-423. https://doi.org/10.3136/nskkk.54.419

Cited by

  1. Cultivation Processes and Yield of Lentinula edodes on Surface Sawdust Bed vol.104, pp.3, 2015, https://doi.org/10.14578/jkfs.2015.104.3.434