Journal of the East Asian Society of Dietary Life (동아시아식생활학회지)

The East Asian Society of Dietary Life (동아시아식생활학회)
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Antibacterial Activity of the Ethanol Extract from Cornus officinalis against Some Bacteria Related to Foodborne Illness and Food Spoilage

산수유 에탄올 추출물의 식중독 및 식품 부패 세균에 대한 항균 활성 분석

  • Jeon, Yeon-Hee (Dept. of Food Science and Nutrition, Kyungpook National University) ;
  • Park, Mi-Hye (Dept. of Food Science and Nutrition, Kyungpook National University) ;
  • Kim, Meera (Dept. of Food Science and Nutrition, Center for Beautiful Aging, Kyungpook National University)
  • 전연희 (경북대학교 식품영양학과) ;
  • 박미혜 (경북대학교 식품영양학과) ;
  • 김미라 (경북대학교 식품영양학과.장수생활과학연구소)
  • Received : 2012.10.10
  • Accepted : 2012.10.30
  • Published : 2012.10.31
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Abstract

The antibacterial activity of the ethanol extract of Cornus officinalis against some bacteria related to foodborne illness and food spoilage was investigated. In the case of the disc diffusion assay, clear zones due to the inhibition of proliferation by Cornus officinalis extract were 8.5~18.3 mm at $4,000{\mu}g/disc$. The inhibitory effect of Cornus officinalis was as follows: Escherichia. coli ${\geq}$ Listeria monocytogenes ${\geq}$ E. coli O157:H7 > Bacillus cereus > Staphylococcus aureus > Pseudomonas aeruginosa > Salmonella typhimurium. In addition, the MIC of Cornus officinalis extract toward B. cereus was the lowest at 1,000 ppm. The extract inhibited the growth of E. coli, L. monocytogenes, E. coli O157:H7, and Bacillus cereus throughout the growth stage. However, the growth of P. aeruginosa, S. aureus, and S. typhimurium was only inhibited by the extract during the lag phase. The constituents of all cells tested were released, especially E. coli and E. coli O157:H7. Observation of the cells using SEM demonstrated a morphological change and disruption of cells in response to treatment with Cornus officinalis extract. Based on these findings, the ethanol extracts of Cornus officinalis showed strong antimicrobial activity against all tested bacteria, indicating that Cornus officinalis can be a useful natural antimicrobial reagent.

Keywords

References

  1. Bauer AW, Kirby WMM, Sherris JC, Turck M (1966) Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol 45: 493-496. https://doi.org/10.1093/ajcp/45.4_ts.493
  2. Beuchat LR, Golden DA (1989) Antimicrobials occurring naturally in foods. Food Tech 43: 134-142.
  3. Brackett RE (1987) Effect of various acids on growth and survival Yersinia enterocolitica. J Food Prot 50: 598-602. https://doi.org/10.4315/0362-028X-50.7.598
  4. Cho GS, Kim HY (2003) Screening of antimicrobial activity from Castanea crenata Sieb. et Zucc. leaves and flowers. II. Screening of antimicrobial activities. J Korean Soc Agric Chem Biotechnol 46: 262-267.
  5. Cho MH, Bae EK, Ha SD, Park JY (2005) Application of natural antimicrobials to food industry. Food Science and Industry 38: 36-45.
  6. Cho SH, Lee HC, Seo IW, Kim ZU (1991) Efficacy of grapefruit seed extract in the preservation of Satsuma mandarin. Korean J Food Sci Technol 23: 614-618.
  7. Chun HJ, Choi WH, Lee JH, Yang HO, Baek SH (2003) Screening of cytotoxicity and antimicrobial effects of hexane extracts from Cornis fructus. Korean J Oriental Physiology & Pathology 17: 476-480.
  8. Chung HY, Jung DH, Park YJ (2000) The screening and pattern comparison of organic acids in 3 kinds of medicinal herbal extracts. Korean J Food Sci Technol 32: 997-1001.
  9. Chung SR, Jeune KH, Park SY, Jang SJ (1993) Toxicity and lectins constituents from the seed of Cornus officinalis. Korean J Pharmacogn 24: 177-182.
  10. Cichewicz RH, Thorpe PA (1996) The antimicrobial properties of chile peppers (Capsicum species) and their use in Nayan medicine. J Ethnopharmacol 52: 61-70. https://doi.org/10.1016/0378-8741(96)01384-0
  11. Cowan MM (1999) Plant products as antimicrobial agents. Clin Microbiol Rev 12: 564-582.
  12. Cursino L, Chartone-Souza E, Nascimento AMA (2005) Synergic interaction between ascorbic acid and antibiotics against Pseudomonas aeruginosa. Brazilian Archives of Biology and Technology 48: 379-384. https://doi.org/10.1590/S1516-89132005000300007
  13. Dabidson PM, Parish ME (1989) Methods for testing the efficacy of food antimicrobials. Food Technol 43:148-155.
  14. Dai Y, Hang B, Huang Z (1992) Inhibition of Cornis fructus on experimental inflammation. China Journal of Chinese Materia Medica 17: 307-309.
  15. Demirci F, Guven K, Demirci B, Dadandi MY, Baser KHC (2008) Antibacterial activity of two Phalomis essential oils against food pathogens. Food Control 19: 1159-1164. https://doi.org/10.1016/j.foodcont.2008.01.001
  16. Jang JS, Lee HJ, Oh BY, Lee JM, Go JM, Kim YH (2007) Inactivation of Escherichia coli O157:H7, Salmonella and Listeria monocytogenes by organic acid. Kor J Env Hlth 33: 403-407.
  17. Jeon YH, Kim MH, Kim MR (2008) Antioxidative, antimutgenic and cytotoxic activities of ethanol extracts from Cornus officianalis. J Korean Soc Food Nutr 37: 1-7. https://doi.org/10.3746/jkfn.2008.37.1.1
  18. Jeon YH, Sun XQ, Kim MR (2012) Antimicrobial activity of the ethanol extract from Rubus coreanum against microorganisms related with foodborne illness. Korean J Food Cookery Sci 28: 9-15. https://doi.org/10.9724/kfcs.2012.28.1.009
  19. Kim HY, Lee YJ, Kim SH, Hong KH, Kwon YK, Lee JY, Ha SC, Cho HY, Chang IS, Lee CW, Kim KS (1999) Studies on the development of natural preservatives from natural products. Korean J Food Sci Techol 31: 1667-1678.
  20. Kim JY, Lee JA, Kim KN, Song GP, Park SY (2007a) Antioxidative and antimicrobial activities of Euphorbia helioscopia extracts. J Korean Soc Food Sci Nutr 36: 1106-1112. https://doi.org/10.3746/jkfn.2007.36.9.1106
  21. Kim JY, Lee JA, Kim KN, Yoon WJ, Lee WJ, Park SY (2007b) Antioxidative and antimicrobial activaties of Sargassum muticum extracts. J Korean Soc Food Sci Nutr 36: 663-669. https://doi.org/10.3746/jkfn.2007.36.6.663
  22. Kim SR, Kim MR (2012) Inhibition of foodborne pathogens and spoilage bacteria and their structural changes by ethanol extract of Schizandra chinensis Baillon. J East Asian Soc Dietary Life 22: 109-119.
  23. Kim YD, Kim HK, Kim KJ (2003a) Analysis of nutritional components of Cornus officianalis. J Korean Soc Food Sci Nutr 32: 785-789. https://doi.org/10.3746/jkfn.2003.32.6.785
  24. Kim YD, Kim HK, Kim KJ (2003b) Antimicrobial activity of solvent fraction from Cornus officianalis. J Korean Soc Food Sci Nutr 32: 829-832. https://doi.org/10.3746/jkfn.2003.32.6.829
  25. Kwak DJ, Nam SY, Lee DS (2002) Antibacterial activity of phellodendri cortex on dental caries bacteria Staphylococcus sanguis. J Kor Aca Den Technol 24: 43-49.
  26. Kwon SH, Yang HS, Kim JY, Park KW, Shon MY, Kang KS, Shim KH, Seo KI (2009) Biological activities of ethanol extract from Cornis fructus. J Korean Soc Food Sci Nutr 38: 287-291. https://doi.org/10.3746/jkfn.2009.38.3.287
  27. Lambert RJW, Skandamis PN, Coote PJ, Nychas GHE (2001) A study of the minimum inhibitory concentration and mode of action of oregano essential oil, thymol and carvacrol. J Appl Microbiol 91: 453-562. https://doi.org/10.1046/j.1365-2672.2001.01428.x
  28. Lee BW, Shin DH (1991) Antimicrobial effect of some plant extracts and their fractionates for food spoilage microorganisms. Korean J Food Sci Technool 23: 205-211.
  29. Lee JJ, Kim SH, Chang BS, Lee JB, Huh CS, Kim TJ, Baek YJ (1999) The antimicrobial activity of medicinal plants extracts against Helicobacter pylori. Korean J Food Sci Technol 31: 764-770.
  30. Lee SO, Han SM, Kim HM, Jeung SK, Choi JY, Kang IJ (2006) Chemical components and antimicrobial effects of Cornis fructus. J Korean Soc Food Sci Nutr 35: 891-896. https://doi.org/10.3746/jkfn.2006.35.7.891
  31. Lee YC, Oh SW, Hong HD (2002) Antimicrobial characteristics of edible medicinal herbs extracts. Korean J Food Sci Technol 34: 700-709.
  32. McFarland J (1907) Nephelometer: an instrument for estimating the number of bacteria in suspensions used for calculating the opsonic index and for vaccines. J Am Med Assoc 14:1176-1178.
  33. Ogden SK, Taylor AJ, Dodd CER, Guerrero I, Buendia HE, Gallardo F (1996) The effect of combining propionic and ascorbic acid on the keeping qualities of fresh minced pork during storage. Lebensm-Wiss u-Technol 29: 227-233. https://doi.org/10.1006/fstl.1996.0033
  34. Oh DH, Ham SS, Park BK, Ahn C, Yu JY (1998) Antimicrobial activities of natural medicinal herbs on the food spoilage or foodborne disease microorganisms. Korean J Food Sci Technol 39: 957-963.
  35. Oh DH, Lee MK, Park BK (1999) Antimicrobial activities of commercially available tea on the harmful foodborne organisms. Korea Soc Food Sci Nutr 28: 100-106.
  36. Park YK, Whang WK, Kim IH (1995) The antidiabetic effects of from Cornus officinalis seed. Chung-Ang J Pham Sci 9: 13-22.
  37. Peres MT, Monache FD, Cruz AB, Pizzolatti MG, Yunes RA (1997) Chemical composition and antimicrobial activity of Croton urucurana Baillon (Euphorbiaceae). J Ethnopharmacol 56: 223-226. https://doi.org/10.1016/S0378-8741(97)00039-1
  38. Ryu YJ (2003) Antimicrobial characteristic of Gardemia jasminoides extract. MS Thesis Gyeongsang National University, Gyeongnam. pp 24-38.
  39. Seo KI, Kang KS, Shim KH (1997) Effects of mustard seed (Brassica juncea) during preservation of soup for Naengmyon. Korean J Food Sci Technol 29: 51-56.
  40. Seo KI, Lee SW, Yang KH (1999) Antimicrobial and antioxidative activities of Corni fructus extracts. Korean J Postharvest Sci Technol 6: 99-103.
  41. Tabak M, Armom R, Potasman I, Neeman I (1996) In vitro inhibition of Helicobacter pylori by extracts of thyme. J Appl Baceriol 80: 667-672.
  42. Tabak M, Armon R, Rosenblot G, Stermer E, Neeman I (2003) Divers effect of ascorbic acid and palmitoyl ascorbate on Helicobacter pylori survival and growth. FEMS Mictobiology Letters 224: 247-253. https://doi.org/10.1016/S0378-1097(03)00439-7
  43. Turgis M, Han JJ, Caillet S, Lacroix M (2009) Antimicrobial activity of mustard essential oil against Escherichia coli O157:H7 and Salmonella typhi. Food Control 20: 1073-1079. https://doi.org/10.1016/j.foodcont.2009.02.001
  44. Wo WS (1995) Research techniques in natural products chemistry. Seoul National University Press, Seoul. pp 10-29.
  45. Yu MH, Chae IG, Jung YT, Jeong YS, Kim HI, Lee IS (2011) Antioxidative and antimicrobial of methanol extract from Rosmarinus officinalis L. and their fractions. J Life Science 21: 375-384. https://doi.org/10.5352/JLS.2011.21.3.375
  46. Zambuchini B, Fiorini D, Verdenelli MC, Orpianesi C, Ballini R (2008) Inhibition of microbiological activity during sole (Solea solea L.) chilled storage by applying ellagic and ascorbic acid. LWT Food Science and Technology 41: 1733-1738. https://doi.org/10.1016/j.lwt.2007.11.004