Horticulture, Environment, and Biotechnology : HEB

Korean Society of Horticultural Science (한국원예학회)
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Isolation of Antibacterial Compounds from Parasenecio pseudotaimingasa

  • Cho, Eun Ju (Department of Food Science and Nutrition, Pusan National University) ;
  • Choi, Ji Yeon (Department of Food Science and Nutrition, Pusan National University) ;
  • Lee, Ki Ho (Department of Integrative Plant Science, Chung-Ang University) ;
  • Lee, Sanghyun (Department of Integrative Plant Science, Chung-Ang University)
  • Received : 2012.03.26
  • Accepted : 2012.10.09
  • Published : 2012.12.31
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Abstract

The aim of this research was to investigate the antibacterial activity of Parasenecio pseudotaimingasa, a perennial plant belonging to the Asteraceae, tribe Senecioneae. The methanol extract, n-hexane fraction, and n-butanol fraction of P. pseudotaimingasa leaves all exhibited antibacterial activities against Escherichia coli and Staphylococcus aureus, forming inhibition zones greater than 11 mm in discs. Among them, the n-hexane fraction showed the highest antibacterial activity against E. coli and S. aureus, with an inhibition zone greater than 14 mm. Open-column chromatography was used to isolate the phytochemical constituents from the n-hexane fraction; spectroscopic analysis elucidated their structures as ${\beta}$-sitosterol and daucosterol. Further testing of ${\beta}$-sitosterol and daucosterol also revealed the antibacterial effects against both E. coli and S. aureus, suggesting their potential as antibacterial agents.

Keywords

References

  1. Agung, N., M.H. Kim, J.H. Lee, J.D. Kim, K.R. Lee, J.S. Choi, Y.M. Yoo, and H.J. Park. 2011. Polyphenol analysis and peroxynitrite scavenging effect of the extracts from eight Korean mountainous vegetable. Kor. J. Pharmacogn. 42:38-45.
  2. Allen, U.D., N. MacDonald, L. Fuite, F. Chan, and D. Stephens. 1999. Risk factors for resistance to "first-line" antimicrobial resistance among urinary tract isolates of Escherichia coli in children. Can. Med. Ass. J. 160:1436-1440.
  3. Baek, N.I., J.T. Han, E.M. Ahn, J.K. Park, S.W. Cho, S.G. Jeon, J.S. Jang, C.K. Kim, and S.Y. Choi. 2000. Isolation of anticonvulsant compounds from the fruits of Schizandra chinensis BAILI. J. Korean Soc. Agric. Chem. Biotechnol. 43:72-77.
  4. Bandow, J.E., H. Brotz, L.I.O. Leichert, H. Labischinski, and M. Hecker. 2003. Proteomic approach to understanding antibiotic action. Antimicrob. Agents Chemother. 47:948-955. https://doi.org/10.1128/AAC.47.3.948-955.2003
  5. Benkeblia, N. 2004. Antimicrobial activity of essential oil extracts of various onions (Allium cepa) and garlic (Allium sativum). Lebensm.-Wiss.u.-Technol. 37:263-268.
  6. Davidson, P.M. and M.E. Parish. 1989. Methods for testing the efficacy of food antimicrobials. Food Technol. 43:148-155.
  7. Jain, S.C., B. Singh, and R. Jain. 2001. Antimicrobial activity of triterpenoids from Heliotropium ellipticum. Fitoterapia 72:666-668. https://doi.org/10.1016/S0367-326X(01)00267-2
  8. Jin, Y.H. and Y.H. Ahn. 2010. Comparison of ecological characteristics of Parasenecio firmus population in Korea and China. J. Environ. Sci. 19:197-207.
  9. Kang, S.J., J.H. Kim, and G.J Joo. 2005. Isolation of antagonistic bacteria against Fusarium oxysporum and physicochemical properties of compost mixed with microbial formulation. Kor. J. Hort. Sci. Technol. 23:342-350.
  10. Karlowsky, J.A., L.J. Kelly, C. Thornsberry, M.E. Jones, and D.F. Sahm. 2002. Trends in antimicrobial resistance among urinary tract infection isolates of Escherichia coli from female outpatients in the United States. Antimicrob. Agents Chemother. 46:2540-2545. https://doi.org/10.1128/AAC.46.8.2540-2545.2002
  11. Kim, D.H., M.J. Song, K.M. Han, M.H. Bang, B.M. Kwon, S.H. Kim, D.K. Kim, I.J. Chung, M.H. Park, and N.I. Baek. 2004. Development of biologically active compounds from edible plant sources-X. Isolation of lipids from the flower of Campsis grandiflora K. Schum. and their inhibitory effect on FPTase. J. Kor. Soc. Appl. Biol. Chem. 47:357-360.
  12. Kim, H.M., J.M. Lee, K.H. Lee, Y.H. Ahn, and S. Lee. 2011. Aldose reductase inhibition by luteolin derivatives from Parasenecio pseudotaimingasa. Nat. Prod. Sci. 17:367-371.
  13. Kim, S.H., D.S. Shin, M.N. Oh, S.C. Chung, J.S. Lee, I.M. Chang, and K.B. Oh. 2003. Inhibition of sortase, a bacterial surface protein anchoring transpeptidase, by $\beta-sitosterol-3-O-glucopyranoside$ from Fritillaria verticillata. Biosci. Biotechnol. Biochem. 67:2477-2479. https://doi.org/10.1271/bbb.67.2477
  14. Koyama, H. 1969. Taxonomic studieson the tribe Senecioneae of Eastern Asia. II. Enumeration of the species of Eastern Asia. Mem. Fac. Sci. Kyoto Univ. Ser. Biol. 2:137-183.
  15. Landgren, M., H. Oden, I. Kuhn, A. Osterlund, and G. Kahlmeter. 2005. Diversity among 2481 Escherichia coli from women with community-acquired lower urinary tract infections in 17 countries. J. Antimicrob. Chemother. 55:928-937. https://doi.org/10.1093/jac/dki122
  16. Lee, J.H., J.Y. Lee, J.H. Park, H.S. Jung, J.S. Kim, S.S. Kang, Y.S. Kim, and Y.M. Han. 2007. Immunoregulatory activity by daucosterol, a $\beta-sitosterol$ glycoside, induces protective Th1 immune response against disseminated Candidiasis in mice. Vaccine 25:3834-3840. https://doi.org/10.1016/j.vaccine.2007.01.108
  17. Lee, J.H., A.R. Park, D.W. Choi, J.D. Kim, J.C. Kim, J.H. Ahn, H.Y. Lee, M. Choe, K.P. Choi, I.C. Shin, and H.J. Park. 2011. Analysis of chemical compositions and electron-donating ability of 4 Korean wild sannamuls. Kor. J. Med. Crop Sci. 19:111-116. https://doi.org/10.7783/KJMCS.2011.19.2.111
  18. Lee, T.B. 2003. Coloured flora of Korea. Hyangmunsa, Seoul, Korea.
  19. Park, E.H., J.H. Kahng, S.H. Lee, and K.H. Shin. 2001. Anantiinflammatory principle from cactus. Fitoterapia 72:288-290. https://doi.org/10.1016/S0367-326X(00)00287-2
  20. Park, H.J., A. Nugroho, J. Lee, J.D. Kim, W.B. Kim, K.R. Lee, and J.S. Choi. 2009. HPLC analysis of caffeoylquinic acids in the extract of Cacalia firma and peroxynitrite scavenging effect. Kor. J. Pharmacogn. 40:365-369.
  21. Park, J.Y., S. Lee, S. Han, H.M. Kim, J.M. Lee, Y.H. Ahn, S.Y. Lee, and S. Lee. 2009. Phytochemical constituents from the seeds of Lithospermum erythrorhizon. Nat. Prod. Sci. 15:181-184.
  22. Rojas, R., B. Bustamante, J. Bauer, I. Fernandez, J. Alban, and O. Lock. 2003. Antimicrobial activity of selected Peruvian medicinal plants. J. Ethnopharmacol. 88:199-204. https://doi.org/10.1016/S0378-8741(03)00212-5
  23. Schmidt, B., D.M. Ribnicky, A. Poulev, S. Logendra, W.T. Cefalu, and I. Raskin. 2008. A natural history of botanical therapeutics. Metabolism 57(Suppl. 1):S3-S9.
  24. Sonohara, R., N. Muramatsu, H. Ohshima, and T. Kondo. 1995. Difference in surface properties between Escherichia coli and Staphylococcus aureus as revealed by electrophoretic mobility measurements. Biophys. Chem. 55:273-277. https://doi.org/10.1016/0301-4622(95)00004-H
  25. Sultana, N., M.M. Rahman, S. Ahmed, S. Akter, M.M. Haque, S. Parveen, and S.M.I. Moeiz. 2011. Antimicrobial compounds from the rihzomes of Sansevieria hyacinthoides. Bangladesh J. Sci. Ind. Res. 46:329-332.
  26. Umlauf, D., J. Zapp, H. Becker, and K.P. Adam. 2004. Biosynthesis of the irregular monoterpene artemisia ketone, the sesquiterpene germacrene D and other isoprenoids in Tanacetum vulgare L. (Asteraceae). Phytochemistry 65:2463-2470. https://doi.org/10.1016/j.phytochem.2004.08.019
  27. Westh, H., C.S. Zinn, and V.T. Rosdahl. 2004. An international multicenter study of antimicrobial consumption and resistance in Staphylococcus aureus isolates from 15 hospitals in 14 countries. Microb. Drug Resist. 10:169-176. https://doi.org/10.1089/1076629041310019
  28. Yuk, J.E., J.S. Woo, C.Y. Yun, J.S. Lee, J.H. Kim, G.Y. Song, E.J. Yang, I.K. Hur, and I.S. Kim. 2007. Effects of $lactose-\beta-sitosterol$ and $\beta-sitosterol$ on ovalbumin-inducedlung inflammation in actively sensitized mice. Int. Immunopharmacol. 7:1517-1527. https://doi.org/10.1016/j.intimp.2007.07.026

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