The Journal of Korean Obstetrics and Gynecology (대한한방부인과학회지)

The Society of Korean Medicine Obstetrics and Gynecology (대한한방부인과학회)
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In Vitro Antibacterial Effects of Wandae-tang Aqueous Extracts and Their Combination Effects with Clindamycin against Gardnerella Vaginalis

완대탕(完帶湯)의 Gardnerella vaginalis에 대한 시험관내 항균력 및 Clindamycin과 병용효과

  • Lee, Seung-Hye (Dept. of Oriental Obstetrics & Gynecology, College of Oriental Medicine, Dae-Gu Hanny University) ;
  • Kim, Dong-Chul (Dept. of Oriental Obstetrics & Gynecology, College of Oriental Medicine, Dae-Gu Hanny University)
  • 이승혜 (대구한의대학교 한의과대학 부인과교실) ;
  • 김동철 (대구한의대학교 한의과대학 부인과교실)
  • Received : 2012.01.26
  • Accepted : 2012.02.07
  • Published : 2012.02.24
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Abstract

Objectives: The object of this study was to observe the in vitro antibacterial effects of Wandae-tang extracts and combination of Wandae-tang extracts and Clindamycin against Gardnerella vaginalis ATCC14018. Methods: Antibacterial activities against Gardnerella vaginalis ATCC14018 of Wandae-tang extracts were detected using standard agar microdilution methods. In addition, the effects on the bacterial growth curve were also monitored at minimal inhibitory concentration(MIC) and $MIC{\times}2$ levels. The combination effects of Wandae-tang extracts with Clindamycin were observed by Checkerboard microtiter assay, and the effects of bacterial growth curve was treated with Wandae-tang extracts MIC+Clindamycin MIC, 1/2 MIC and 1/4 MIC, respectively. Results: MIC of Wandae-tang extracts and Clindamycin against Gardnerella vaginalis ATCC14018 were detected as $1.719{\pm}0.856$(0.782~3.125) $mg/m{\ell}$ and $0.010{\pm}0.006$ (0.004~0.016) ${\mu}g/m{\ell}$. In addition, Clindamycin and Wandae-tang extracts were also showed marked dosage-dependent inhibition of bacterial growth, and more dramatical inhibitions were detected in Clindamycin+Wandae-tang extracts MIC treatment. Fractional inhibitory concentration index in combination of Wandae-tang extracts and Clindamycin were detected as $0.294{\pm}0.052$(0.250~0.375) at Checkerboard microtiter assay. Conclusions: The results obtained in this study suggest that Wandae-tang extracts showed antibacterial effects against Gardnerella vaginalis ATCC14018, and they also showed dosage-dependent inhibitory effects on the bacterial growth. In addition, combination treatment of Wandae-tang extracts with Clindamycin showed more synergistically potent inhibitory effects on the growth of Gardnerella vaginalis.

Keywords

References

  1. Abraham Rubin. Vaginitis in Manual of clinical problems in OBGY. Boston: Little, Brown and Company. 1990:285.
  2. Aroutcheva AA et al. Gardnerella vaginalis isolated from patients with bacterial vaginosis and from patients with healthy vaginal ecosystems. Clin Infect Dis. 2001;33(7):1022-7. https://doi.org/10.1086/323030
  3. Eschenbach DA et al. Diagnosis and clinical manifestations of bacterial vaginosis. Am J Obstet Gynecol. 1988;158(4):819-28. https://doi.org/10.1016/0002-9378(88)90078-6
  4. 이정규. Gardnerella vaginalis의 성장조건과 Biotypes. 대한산부인과학회지. 1993;36(6):837-46.
  5. 대한산부인과학회. 부인과학. 서울:칼빈서적. 1997:256-62.
  6. Leitivh H, Bodner-Adler B. Bacterial vaginosis as a risk factor for Preterm delivery; meta-analysis. Am J Obstet Gynecol. 2003;189(1):139-47. https://doi.org/10.1067/mob.2003.339
  7. Hashemi FB et al. Activation of human immunodeficiency virus type 1 expression by Gardnerella vaginalis. J Infect Dis. 1999;179(4):924-30. https://doi.org/10.1086/314674
  8. 김연희, 이흥식. Gardnerella vaginalis에 대한 한약재의 항균활성. 한국미생물.생명공학회지. 2006;34(1):70-3.
  9. 장경호, 이동녕, 김형준. Gardnerella vaginalis 에 대한 목단피, 소목, 어성초, 연교, 하고초, 현삼의 항균효과. 동의생리병리학회지. 2005;9(4):1016-21.
  10. 傅山. 傅靑主女科. 上海:人民出版社. 1978:1-6.
  11. 국윤범 등. 방제학. 서울:영림사. 2008:373-5.
  12. 송석호 등. 완대탕이 이뇨, 소염 및 항균에 미치는 영향. 경희한의대논문집. 1989;12(1):337-47.
  13. Pfaller MA et al. Collaborative investigation of variables in susceptibility testing of yeasts. Antimicrob Agents Chemother. 1990;34(9):1648-54. https://doi.org/10.1128/AAC.34.9.1648
  14. Pfaller MA et al. Standardized susceptibility testing of fluconazole: an international collaborative study. Antimicrob Agents Chemother. 1992;36(9):1805-9. https://doi.org/10.1128/AAC.36.9.1805
  15. Tenover FC et al. Characterization of staphylococci with reduced susceptibilities to vancomycin and other glycopeptides. J Clin Microbiol. 1998;36(4):1020-7.
  16. Pfaller MA et al. Multicenter evaluation of four methods of yeast inoculum preparation. J Clin Microbiol. 1988;26(8):1437-41.
  17. Janssen AM, Scheffer JJ, Baerheim Svendsen A. Antimicrobial activity of essential oils: a 1976-1986 literature review. Aspects of the test methods. Planta Med. 1987;53(5):395-8. https://doi.org/10.1055/s-2006-962755
  18. Isenberg HD. Synergism testing: Broth microdilution checkerboard and broth macrodilution methods. In: Clin Microbiol procedure manual. Washington, DC: American Society for Microbiology. 1992:1-28.
  19. 윤정숙, 문희원, 이미애. 다제내성 Pseudomonas aeruginosa 균주의 항균제 병합효과. 대한임상미생물학회지. 2006;9(1):1-6.
  20. 한의부인과학 교재편찬위원회. 한의부인과학. 서울:도서출판 정담. 2002:260-302.
  21. 송병기. 한방부인과학. 서울:행림출판사. 1994:220-39.
  22. 上海中醫學院編. 婦産科學. 香港:商務印書館. 1997:92-9.
  23. Bannatyne RM, Smith AM. Recurrent bacterial vaginosis and metronidazole resistance in Gardnerella vaginalis. Sex Transm Infect. 1998;74(6):455-6.
  24. Hay PE. Therapy of bacterial vaginosis. J Antimicrob Chemother. 1998;41(1):6-9. https://doi.org/10.1093/jac/41.1.6
  25. Pigrau C et al. Osteomyelitis of the jaw: resistance to clindamycin in patients with prior antibiotics exposure. Eur J Clin Microbiol Infect Dis. 2009;28(4):317-23. https://doi.org/10.1007/s10096-008-0626-z
  26. Mongkolrattanothai K et al. Epidemiology of community-onset Staphylococcusaureus infections in pediatric patients: an experience at a Children's Hospital in central Illinois. BMC Infect Dis. 2009;9:112. https://doi.org/10.1186/1471-2334-9-112
  27. Ajantha GS et al. Phenotypic detection of inducible clindamycin resistance among Staphylococcus aureus isolates by using the lower limit of recommended inter-disk distance. Indian J Pathol Microbiol. 2008;51(3):376-8. https://doi.org/10.4103/0377-4929.42515
  28. Braga LC et al. Synergic interaction between pomegranate extract and antibiotics against Staphylococcus aureus. Can J Microbiol. 2005;51(7):541-7. https://doi.org/10.1139/w05-022
  29. Choi JG et al. In vitro activity of methyl gallate isolated from Galla rhois alone and in combination with ciprofloxacin against clinical isolates of salmonella. J Microbiol Biotechnol. 2008;18(11):1848-52.
  30. Shahverdi AR et al. Galbanic acid from Ferulaszowitsiana enhanced the antibacterial activity of penicillin G and cephalexin against Staphylococcus aureus. Biol Pharm Bull. 2007;30(9):1805-7. https://doi.org/10.1248/bpb.30.1805
  31. 성현아, 박영선, 김동철. 蛇床子洗方의 Gardnerella vaginalis 에 대한 시험관 내 항균력 및 Metronidazole과 병용효과. 대한한방부인과학회지. 2010;23(2):20-37.
  32. 권지명, 김동철. 淸帶湯의 Gardnerella vaginalis 에 대한 시험관내 항균력 및 Clindamycin과의 병용효과. 대한한방부인과학회지. 2011;24(2):1-12.
  33. Thomas C, Stevenson M, Riley TV. Antibiotics and hospital-acquired Clostridium difficile-associated diarrhoea: a systematic review. J Antimicrob Chemother. 2003;51(6):1339-50. https://doi.org/10.1093/jac/dkg254
  34. Altrichter T, Heizmann WR. Gardnerella vaginalis: transport, microscopy, testing resistance. Geburtshilfe Frauenheilkd. 1994;54(11):606-11. https://doi.org/10.1055/s-2007-1022350
  35. Muli F, Struthers JK. Use of a continuous-culture biofilm system to study the antimicrobial susceptibilities of Gardnerella vaginalis and Lactobacillus acidophilus. Antimicrob Agents Chemother. 1998;42(6):1428-32.
  36. Frimodt-Moller N. Correlation of in vitro activity and pharmacokinetic parameters with effect in vivo for antibiotics. Observations from experimental pneumococcus infection. Dan Med Bull. 1988;35(5):422-37.
  37. 최은영 등. 백출 추출물의 세포독성과 항균효과검색. 동의병리학회지. 2002;16(2):348-52.
  38. Kum EJ et al. Antifungal Activity of Phenanthrene Derivatives from Aerial Bulbils of Dioscorea batatas Decne. J Life Sci. 2006;16(4):647-52. https://doi.org/10.5352/JLS.2006.16.4.647
  39. Bae EA et al. In vitro anti-Helicobacter pylori activity of panaxytriol isolated from ginseng. Arch Pharm Res. 2001;24(4):297-9. https://doi.org/10.1007/BF02975095
  40. 한영실, 전희정, 황재선. 백작약으로부터 식품부패 미생물에 대한 항균성 물질의 분리 및 동정. 한국조리과학회지. 2000;16(5):67-74.
  41. 박부길 등. 식품부패 및 병원성미생물에 대한 천연약용식물 추출물의 항균효과. 한국식품과학회지. 1998;30(4):957-63.
  42. Fukai T et al. Antimicrobial activity of licorice flavonoids against methicillin-resistant Staphylococcus aureus. Fitoterapia. 2002;73(6):536-9. https://doi.org/10.1016/S0367-326X(02)00168-5
  43. He J et al. Antibacterial compounds from Glycyrrhiza uralensis. J Nat Prod. 2006;69(1):121-4. https://doi.org/10.1021/np058069d
  44. Yang C et al. Determination of glycyrrhizic acid in different decoctions of sanaotang by HPLC and comparison with antifugal effects in vitro. Zhongguo Zhong Yao Za Zhi. 2007;32(11):1031-4.
  45. 정희경 등. Phytobiotic 소재 선발을 위한 약용식물 추출물의 항산화 및 항균 활성. 한국식품영양과학회지. 2007;36(10):1235-40.
  46. 윤양식 등. 한약재 추출물에 의한 Helicobacter pylori의 생장 및 Urease 활성 억제. 한국생물공학회지. 2004;19(3):187-91.