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Monitoring of Ochratoxin in Alcoholic Beverages

주류의 오크라톡신 모니터링

  • Lee, Joon-Goo (Food Safety Evaluation Department, National Institute of Food & Drug Safety Evaluation) ;
  • Kang, Young-Woon (Food Safety Evaluation Department, National Institute of Food & Drug Safety Evaluation) ;
  • Jeong, Ji-Hye (Hazardous Substances Analysis Division, Gyeongin Regional KFDA) ;
  • Noh, Mi-Jung (Hazardous Substances Analysis Division, Gyeongin Regional KFDA) ;
  • Ahn, En-Sook (Hazardous Substances Analysis Division, Gyeongin Regional KFDA) ;
  • Lee, Kwang-Ho (Food Safety Evaluation Department, National Institute of Food & Drug Safety Evaluation) ;
  • Kim, Mee-Hye (Food Safety Evaluation Department, National Institute of Food & Drug Safety Evaluation)
  • 이준구 (식품의약품안전평가원 식품위해평가부) ;
  • 강영운 (식품의약품안전평가원 식품위해평가부) ;
  • 정지혜 (경인지방식품의약품안전청 유해물질분석과) ;
  • 노미정 (경인지방식품의약품안전청 유해물질분석과) ;
  • 안은숙 (경인지방식품의약품안전청 유해물질분석과) ;
  • 이광호 (식품의약품안전평가원 식품위해평가부) ;
  • 김미혜 (식품의약품안전평가원 식품위해평가부)
  • Received : 2011.06.15
  • Accepted : 2012.01.01
  • Published : 2012.04.30

Abstract

This research was conducted to monitor ochratoxin A in wine, beer, $makgeolli$ and fermented alcoholic beverages to estimate the exposure to ochratoxin A in the assorted alcoholic beverages. The analytical method for ochratoxin A was based on immuno-affinity column clean up followed by HPLC-FLD. Ochratoxin A was detected in 30 samples of 177 wine (17%), 25 samples of 106 beer (23.6%), 11 samples of 74 $makgeolli$ (14.9%), and 7 samples of 74 fermented alcoholic beverages (9.5%). The average levels of ochratoxin A were 0.039 ng/mL in wine, 0.010 ng/mL in beer, 0.023 ng/mL in $makgeolli$, and 0.014 ng/mL in fermented alcoholic beverages. The daily dietary exposure level of ochratoxin A estimated by using the report on national health and nutrition survey were 0.039 ng/b.w.day from wine, 0.010 ng/b.w.day from beer, 0.023 ng/b.w.day from $makgeolli$, and 0.014 ng/b.w.day from fermented alcoholic beverage.

본 연구를 통해 주류 중 와인, 맥주, 곡주(막걸리), 과실주 및 약주의 오크라톡신 A 오염 실태를 조사하였으며, 와인은 0.21 ng/mL, 맥주는 0.028 ng/mL, 곡주(막걸리)는 0.18 ng/mL, 과실주 및 약주는 0.18 ng/mL의 평균 검출량을 보였으며, EU에서 설정하고 있는 기준인 2.0 ng/mL보다 훨씬 낮은 수준이었다. 하지만 우리나라의 기후와 지역의 특성을 고려하여 고온 다습한 남쪽지역에서 생산되는 주류의 오크라톡신 A에 대한 오염실태 조사가 조금 더 이루어져야 할 것으로 생각된다. 또한 주류를 통한 오크라톡신 A의 섭취추정량 0.0008 ng/b.w.day는 EU에서 권고하는 오크라톡신 A 일일섭취한계량(TDI) 중 주류를 통해 섭취하는 오크라톡신 A의 TDI값의 4.5%인 0.2 ng/b.w.day에 비하여 훨씬 적은 양으로 우리나라는 주류에 의한 오크라톡신 A에 대하여 안전하다고 판단된다.

Keywords

References

  1. Van der Merwe KJ, Steyn PS, Fourje S, Scott DB, Theron JJ. Ochratoxin A, a toxic metabolite produced by Aspergillus ochraceus Wilh. Nature 205: 1112-1113 (1965) https://doi.org/10.1038/2051112a0
  2. Lau BPY, Scott PM, Lewis DA, Kanhere SR. Quantitative determination of ochratox A in by liquid chromatography/electrospray tandem mass spectrometry. J. Mass Spectrom. 35: 23-32 (2000) https://doi.org/10.1002/(SICI)1096-9888(200001)35:1<23::AID-JMS903>3.0.CO;2-B
  3. Krogh. Role of ochratoxin A in disease causation. Food Chem. Toxicol. 10: 213-244 (1992)
  4. Casegnaro M, Wild CP. IARC activities in mycotoxin research. Nat. Toxins 3: 327-331 (1995) https://doi.org/10.1002/nt.2620030431
  5. Park JW, Choi SY, Hwang HJ, Kim YB. Fungal mycoflora and mycotoxins in Korean polished rice destined to humans. Int. J. Food Microbiol. 103: 305-314 (2005) https://doi.org/10.1016/j.ijfoodmicro.2005.02.001
  6. Yoshiko SK, Masahiro N, Setsuko T, Eiichi I, Toshitsugu T, Hiroko N, Yoshinori I, Koji A, Kazuhiro F, Shigeni K, Susumu K. Occurrence of aflatoxins, ochratoxin A, and fumonisins in retail foods in Japan. J. Food Protect. 69: 1365-1370 (2006) https://doi.org/10.4315/0362-028X-69.6.1365
  7. Sangare TB, Bem AA, Kouadio HJ, Betbeder AM, Dano DS, Moukha S, Creppy EE. Preliminary survey of ochratoxin A in millet, maize, rice and peanuts in Coted'Ivoire from 1988 to 2002. Hum. Exp. Toxicol. 25: 211-216 (2006) https://doi.org/10.1191/0960327106ht605oa
  8. Czerwiechi L, Czajkowska D, Witkowska GA. On ochratoxin A and fungal flora in polish cereals from conventional and ecological farms. Part 2: Occurrence of ochratoxin A and fungi in cereals in 1998. Food Addit. Contam. 19: 1051-1057 (2002) https://doi.org/10.1080/02652030210156313
  9. Birzele B, Prange A, Kramer J. Deoxynivalenol and ochratoxin A in German wheat and changes of level in relation to storage parameters. Food Addit. Contam. 17: 1027-1035 (2000) https://doi.org/10.1080/02652030050207828
  10. Fazekas B, Tar AK, Zomborszky KM. Ochratoxin A contamination of cereal grains and coffee in hungrary in the year 2001. Acta Vet. Hung. 50: 177-188 (2001)
  11. Park JW, Kim EK, Shon DH, Kim YB. Natural co-occurrence of aflatoxin B1, fumonisin B1, and ochratoxin A in barley and corn foods from Korea. Food Addit. Contam. 19: 1073-1080 (2002) https://doi.org/10.1080/02652030210151840
  12. Park SK, Kwon KS, Kim MH, Jeong SY, Jang GH, Nam TH, Lee JO, Kim MC. Survey of ochratoxin A in cereal-based Korean traditional foods by HPLC. Korean J. Food Sci. Technol. 36: 158-161 (2004)
  13. Park JW, Chung SH, Kim YB. Ochratoxin A in Korean food commodities: Occurrence and safety evaluation. J. Agr. Food Chem. 53: 4637-4642 (2005) https://doi.org/10.1021/jf050227j
  14. Chung SH, Lee KH. Occurence of mycotoxins in food. The annual report of KFDA. 10: 673-674 (2006)
  15. Keith AS, Susan P, Victor B. Surveillance of stored grain from the 1997 harvest in United Kingdom for ochratoxin A. Food Addit. Contam. 16: 281-290 (1999) https://doi.org/10.1080/026520399283948
  16. Angelo V, Michelangelo P, Gianluca C. Determination of ochratoxin A in wine and beer by immunoaffinity column cleanup and liquid chromatographic analysis with fluorometric detection. Colaborative study. J. AOAC Int. 84: 1818-1827 (2001)
  17. Park JW. Revision of the analysis of makgeolli for ochratoxin A. Korean J. Food Sci. Technol. 38: 140-142 (2006)
  18. Roussea J. Ochratpxin A in wines: Current knowledge. Vinidea. net Wine Internet Tech. J. 5: 1-5 (2004)

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