Journal of the Korean Society of International Agriculture (한국국제농업개발학회지)

The Korean Society of International Agriculture (한국국제농업개발학회)
권호 표지

Effect on Insecticide Susceptibility of Lissorhoptrus oryzophilus Fed on Bacillus thuringiensis (Bt)-Transgenic Rice Variety

해충저항성 Bacillus thuringiensis (Bt) 벼 급이가 벼물바구미(Lissorhoptrus oryzophilus)의 살충제 감수성에 미치는 영향

  • Oh, Sung-Dug (National Academy of Agricultural Science, Rural Development Administration) ;
  • Lee, Kijong (National Academy of Agricultural Science, Rural Development Administration) ;
  • Sohn, Soo-In (National Academy of Agricultural Science, Rural Development Administration) ;
  • Kwon, Yong-Jung (Division of Plant Biosciences, School of Plant Biosciences, Kyungpook National University) ;
  • Kim, Jin-Seo (Division of Plant Biosciences, School of Plant Biosciences, Kyungpook National University) ;
  • Lee, Jang-Yong (National Academy of Agricultural Science, Rural Development Administration) ;
  • Park, Beom-Seok (National Academy of Agricultural Science, Rural Development Administration) ;
  • Kweon, Soon-Jong (National Academy of Agricultural Science, Rural Development Administration) ;
  • Suh, Seok-Choel (National Academy of Agricultural Science, Rural Development Administration) ;
  • Ryu, Tae-Hun (National Academy of Agricultural Science, Rural Development Administration) ;
  • Park, Jong-Sug (National Academy of Agricultural Science, Rural Development Administration) ;
  • Ahn, Byung-Ohg (National Academy of Agricultural Science, Rural Development Administration) ;
  • Cho, Hyun-Suk (National Academy of Agricultural Science, Rural Development Administration) ;
  • Suh, Sang-Jae (Division of Plant Biosciences, School of Plant Biosciences, Kyungpook National University)
  • 오성덕 (농촌진흥청 국립농업과학원 농업생명자원부) ;
  • 이기종 (농촌진흥청 국립농업과학원 농업생명자원부) ;
  • 손수인 (농촌진흥청 국립농업과학원 농업생명자원부) ;
  • 권용정 (경북대학교 농업생명과학대학 응용생명과학부) ;
  • 김진서 (경북대학교 농업생명과학대학 응용생명과학부) ;
  • 이장용 (농촌진흥청 국립농업과학원 농업생명자원부) ;
  • 박범석 (농촌진흥청 국립농업과학원 농업생명자원부) ;
  • 권순종 (농촌진흥청 국립농업과학원 농업생명자원부) ;
  • 서석철 (농촌진흥청 국립농업과학원 농업생명자원부) ;
  • 류태훈 (농촌진흥청 국립농업과학원 농업생명자원부) ;
  • 박종석 (농촌진흥청 국립농업과학원 농업생명자원부) ;
  • 안병옥 (농촌진흥청 국립농업과학원 농업생명자원부) ;
  • 조현석 (농촌진흥청 국립농업과학원 농업생명자원부) ;
  • 서상재 (경북대학교 농업생명과학대학 응용생명과학부)
  • Published : 2012.06.30
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Abstract

Insect-resistant transgenic rice, Bt rice was developed by inserting the mCry1Ac1, a modified gene from the soil bacterium Bacillus thuringiensis (Bt). For the biosafety assessment of this GM rice, we studied the effect on insecticide susceptibility of Rice Water Weevil (Lissorhoptrus oryzophilus) which is a nontarget insect pest and commonly used as a model organism in ecotoxicological studies. The Bt rice was confirmed to have the insertion of T-DNA and the protein expression by the polymerase chain reaction (PCR) and the ELISA analysis. As for the cumulative mortalities of Rice Water Weevil on insecticide, Clothianidin in Bt and non-GM counterpart, Nakdong rices were similar. The 72h-$LC_{50}$ values of Bt and non-GM rices showed 0.245 ml/L and 0.257 ml/L respectively. As the 72h-$LC_{50}$ vlaue of Bt rice was included within the 95% confidence limit of that of Nakdong rice slightly higher, the two varieties of the 72h-$LC_{50}$ values were not significant. Thus, we found out there was a strong possibility that Bt rice didn't affect the insecticide susceptibilities of nontarget insects.

해충저항성 Bacillus thuringiensis (Bt) 벼의 비표적곤충인 벼물바구미(Lissorhoptrus oryzophilus)에 대한 성충 전용살충제 Clothianidin 액상수화제의 살충제 감수성 시험을 실시한 결과, 72시간-$LC_{50}$은 0.245 ml/L(95% 신뢰한계는 0.195~0.307 ml/L) 이었으며, Bt벼의 모본으로 대조로 사용한 낙동벼의 72시간-$LC_{50}$은 0.257 ml/L(95% 신뢰한계는 0.199~0.331 ml/L)이었다. 72시간-$LC_{50}$은 낙동벼에서 다소 높았지만, 해충저항성 Bt벼 72 시간-$LC_{50}$이 낙동벼의 95% 신뢰한계 내에 포함되어, 두 품종 의 $LC_{50}$값에 유의성이 없는 것으로 판단된다.

Keywords

References

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