Laboraroty Animal Research

Korean Association for Laboratory Animal Science (한국실험동물학회)
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Effects of Red Liriope platyphylla on NGF secretion ability, NGF receptor signaling pathway and ${\gamma}$-secretase components in NSE/hAPPsw transgenic mice expressing Alzheimer's Disease

  • Choi, Sun-Il (College of Natural Resources & Life Science, Pusan National University) ;
  • Goo, Jun-Seo (College of Natural Resources & Life Science, Pusan National University) ;
  • Kim, Ji-Eun (College of Natural Resources & Life Science, Pusan National University) ;
  • Hwang, In-Sik (College of Natural Resources & Life Science, Pusan National University) ;
  • Lee, Hye-Ryun (College of Natural Resources & Life Science, Pusan National University) ;
  • Lee, Young-Ju (College of Natural Resources & Life Science, Pusan National University) ;
  • Son, Hong-Joo (College of Natural Resources & Life Science, Pusan National University) ;
  • Lee, Hee-Seob (College of Human Ecology, Pusan National University) ;
  • Lee, Jong-Sup (Nonghyup Sandong Processing Plant) ;
  • Hwang, Dae-Youn (College of Natural Resources & Life Science, Pusan National University)
  • Published : 2012.09.30
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Abstract

Liriope platyphylla (LP) has long been regarded as a curative herb for the treatment of diabetes, asthma, and neurodegenerative disorders. To examine the therapeutic effects of Red LP (RLP) manufactured by steaming process on neurodegenerative disorders, significant alteration of the key factors influencing Alzheimer's Disease (AD) was detected in NSE/hAPPsw transgenic (Tg) mice after RLP treatment. The concentration of nerve growth factor (NGF) in serum increased in RLP-treated NSE/hAPPsw Tg mice compared with vehicle-treated Tg mice. However, downstream effectors of the NGF receptor signaling pathway, including TrkA and $p75^{NTR}$ proteins, were suppressed in RLP-treated NSE/hAPPsw Tg mice. Especially, Tg mice showed decreased levels of TrkA, $p75^{NTR}$, and RhoA expression. Production of A${\beta}$-42 peptides was lower in RLP-treated NSE/hAPPsw Tg mice than in vehicle-treated Tg mice. Further, analysis of ${\gamma}$-secretase components showed that A${\beta}$-42 peptide expression was downregulated. Of the four components, the expression of APH-1 and Nicastrin (NCT) decreased in RLP-treated NSE/hAPPsw Tg mice, whereas expression of PS-2 and Pen-2 was maintained or increased within the same group. Overall, these results suggest that RLP can help relieve neurodegenerative diseases, especially AD, through upregulation of NGF secretion ability, activation of NGF signaling pathway, downregulation of A${\beta}$-42 peptide deposition, and alteration of ${\gamma}$-secretase components.

Keywords

References

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