Food Engineering Progress (산업식품공학)

Korean Society for Industrial Food Engineering (한국산업식품공학회)
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Optimization of Lateral Flow Assay for the Dual-detection of Mycotoxins

곰팡이 독소 동시 분석용 간이진단키트 최적화 연구

  • Moon, Jihea (Department of Agricultural Engineering, National Academy of Agricultural Sciences) ;
  • Kim, Giyoung (Department of Agricultural Engineering, National Academy of Agricultural Sciences) ;
  • Lee, Sangdae (Department of Agricultural Engineering, National Academy of Agricultural Sciences) ;
  • Park, Saetbyeol (Department of Agricultural Engineering, National Academy of Agricultural Sciences) ;
  • Kim, Hyeokju (Department of Agricultural Engineering, National Academy of Agricultural Sciences)
  • 문지혜 (국립농업과학원 농업공학부) ;
  • 김기영 (국립농업과학원 농업공학부) ;
  • 이상대 (국립농업과학원 농업공학부) ;
  • 박샛별 (국립농업과학원 농업공학부) ;
  • 김혁주 (국립농업과학원 농업공학부)
  • Published : 2012.08.31
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Abstract

This study was performed to develop and optimize a rapid strip kit for the dual-detection of Aflatoxin B1 (AF) and Ochratoxin A (OTA). The strip kit is composed of sample pad, conjugation pad, nitrocellulose membrane, and absorbent pad. Manually spotted mycotoxin-BSA conjugates and anti-mouse IgG on nitrocellulose membrane were used as the test line and the control line, respectively. Conditions for rapid and easy detection of mycotoxins, conjugation between toxin-MAbs and nano Au particles, pretreatment method of pads, and composition of standards solutions were optimized. Feasibility of the strip kit to detect AF and OTA were evaluated with standard samples. Test results were acquired in 10 min and the detection limits were 100 ppm for AF and OTA, respectively.

Keywords

References

  1. Akande KE, Abubakar MM, Adegbola TA, Bogoro SE. 2006. Nutritional and health implications of mycotoxins in animal feeds: A review. Pakistan J. Nut. 5: 398-403. https://doi.org/10.3923/pjn.2006.398.403
  2. Bazin I, Nabais E, Lopez-Ferber M. 2010. Rapid visual tests: Fast and reliable detection of ochratoxin A. Toxins. 2: 2230-2241. https://doi.org/10.3390/toxins2092230
  3. Delmulle BS, De Saeger SMDG, Sibanda L, Barna-Vetro I, Van Peteghem CH. 2005. Development of an immunoassay-based lateral flow dipstick for the rapid detection of aflatoxin $B_1$ in pig feed. J. Agric. Food Chem. 53: 3364-3368. https://doi.org/10.1021/jf0404804
  4. Kim DH. 2010. Mycotoxin contamination of feed domestic distribution survey. Monthly poultry. 5: 150-152.
  5. Kim GY, Yang GM, Park SB, Kim YH, Lee KJ, Son JY, Kim HJ, Lee SR. 2011. Rapid detection kit for Salmonella typhimurium. J. of Biosystems Eng. 36: 140-146. https://doi.org/10.5307/JBE.2011.36.2.140
  6. Kolosova AY, Shim WB, Yang ZY, Eremin SA, Chung DH. 2006. Direct competitive ELISA based on a monoclonal antibody for detection of aflatoxin $B_1$. Stabilization of ELISA kit components and application to grain samples. Anal. Bioanal. Chem. 384: 286-294. https://doi.org/10.1007/s00216-005-0103-9
  7. Krska R, Schubert-Ullrich P, Molinellli A, Sulyok M, Macdonald S, Crews C. 2008. Food Addit. Contam. Part A. Chem Anal. Control Expo. Risk Assess. 25: 152-163. https://doi.org/10.1080/02652030701765723
  8. Manda G, Bardhan M, Ganguly T. 2010. Interaction of bovine serum albumin and albumin-gold nanoconjugates with L-aspartic acid. A spectroscopic approach. Colloid. Surf. Biointerfaces. B. 81: 178-184. https://doi.org/10.1016/j.colsurfb.2010.07.002
  9. Moon JH, Kim GY, Lee SD. 2012. A gold nanoparticle and aflatoxin $B_1$-BSA conjugates based lateral flow assay method for the analysis of aflatoxin $B_1$. Materials. 5: 634-643. https://doi.org/10.3390/ma5040634
  10. Oh KS, Choi EJ, Yoo SY, Lee CN, Hong HW. 2011. Status of the inspection for mycotoxins in foods. Food Sci. Indust. 44: 44-49.
  11. Shim WB, Yang ZY, Kim JS, Kim JY, Kang SJ, Woo GJ, Chung YC, Eremin SA, Chung DH. 2007. Development of immunochromatography strip-test using nanocolloidal gold-antibody probe for the rapid detection of aflatoxin $B_1$ in grain and feed samples. J. Microbiol. Biotehnol. 17: 1629-1637.
  12. Turner NW, Subrahmanyam S, Piletsky SA. 2009. Analytical methods for determination of mycotoxins: A review. Anal. Chim. Acta. 632: 168-180. https://doi.org/10.1016/j.aca.2008.11.010
  13. Ye Y, Zhou Y, Mo Z, Cheng W, Yang S, Wang X, Chen F. 2010. Rapid detection of aflatoxin B1 on membrane by dot-immunogold filtration assay. Talanta. 81: 792-798. https://doi.org/10.1016/j.talanta.2010.01.017
  14. Zheng MZ, Richard JL, Binder J. 2006. A review of rapid methods for the analysis of mycotoxins. Mycopathologia. 161: 261- 273. https://doi.org/10.1007/s11046-006-0215-6