Journal of Biosystems Engineering

Korean Society for Agricultural Machinery (한국농업기계학회)
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Rapid Detection Kit for Salmonella typhimurium

살모넬라 식중독균 신속 검출용 간이 진단키트

  • Received : 2011.03.11
  • Accepted : 2011.04.05
  • Published : 2011.04.25
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Abstract

This study was performed to develop a rapid test kit for pathogenic Salmonella in various samples. The rapid detection kit has been fabricated based on nitrocellulose lateral-flow strip. Colloidal gold and biotin conjugated Salmonella antibodies were used as a tag and a receptor, respectively. Manually spotted Salmonella antibody and Neutravidin on nitrocellulose membrane were used as test and control lines, respectively. Feasibility of the rapid kit to detect Salmonella typhimurium in samples were evaluated. The intensity of the color of the test line started to increase with the samples in which higher concentration of the cells were contained. The sensitivity of the sensor was $10^6$ cfu/mL Salmonella spiked in PBS. Also, the rapid test kit could detect $10^6$ cfu/mL of Salmonella in chicken meat extract.

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