Fisheries and Aquatic Sciences

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Isolation and Characterization of Nonylphenol-degrading Bacteria

  • Yu, Dae-Ung (Department of Food Science and Technology, Pukyong National University) ;
  • Kim, Dong-Myung (Department of Ecological Engineering, Pukyong National University) ;
  • Chung, Yong-Hyun (Department of Ecological Engineering, Pukyong National University) ;
  • Lee, Yang-Bong (Department of Food Science and Technology, Pukyong National University) ;
  • Kim, Young-Mog (Department of Food Science and Technology, Pukyong National University)
  • Received : 2012.02.13
  • Accepted : 2012.03.02
  • Published : 2012.06.30
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Abstract

To isolate a nonylphenol (NP)-degrading bacterium, we isolated a single colony from the NP-degrading microbial consortium SW-3, which was previously isolated from an aqueous environment. Ten colonies that exhibited different cell morphologies were isolated and the strains were named SW-3-A, -B, -C, -D, -E, -F1, -F2, -G, -H, and -I. The ability of isolates to degrade NP was evaluated by kinetic analysis by the constant of NP degradation rate ($k_1$) and the half-life time of NP degradation ($t_{1/2}$). SW-3-F1, -F2, -G, and -I strains were superior at degrading NP. The $k_1$ and $t_{1/2}$ values of the four strains were sixfold higher and one-sixth lower, respectively, than those of the consortium strain. Additionally, SW-3-F1, -G, and -I strains were tested for their ability to degrade NP during coculture. NP degradation by coculture with a combination of all three strains was inferior to that of culture conducted with single isolates, suggesting that the three strains are antagonistic toward each other during NP degradation.

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References

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