한국토양비료학회지 (Korean Journal of Soil Science and Fertilizer)

  • 제45권2호
  • /
  • Pages.169-176
  • /
  • 2012
  • /
  • 0367-6315(pISSN)
  • /
  • 2288-2162(eISSN)
한국토양비료학회 (Korean Society of Soil Science and Fertilizer)
권호 표지
DOI QR코드

DOI QR Code

인산가용화 미생물에 의한 토양 내 인산이온 가용화 기작

Mechanisms of Phosphate Solubilization by PSB (Phosphate-solubilizing Bacteria) in Soil

  • 이강국 (충남대학교 농업생명과학대학 생물환경화학과) ;
  • 목인규 (충남대학교 농업생명과학대학 생물환경화학과) ;
  • 윤민호 (충남대학교 농업생명과학대학 생물환경화학과) ;
  • 김혜진 (충남대학교 농업생명과학대학 생물환경화학과) ;
  • 정덕영 (충남대학교 농업생명과학대학 생물환경화학과)
  • Lee, Kang-Kook (Dept. of Bio-environmental Chemistry, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Mok, In-Kyu (Dept. of Bio-environmental Chemistry, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Yoon, Min-Ho (Dept. of Bio-environmental Chemistry, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Kim, Hye-Jin (Dept. of Bio-environmental Chemistry, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Chung, Doug-Young (Dept. of Bio-environmental Chemistry, College of Agriculture and Life Sciences, Chungnam National University)
  • 투고 : 2012.02.27
  • 심사 : 2012.03.15
  • 발행 : 2012.04.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Among the major nutrients, phosphorus is by far the least mobile and available to plants in most soil conditions. A large portion of soluble inorganic phosphate applied to soil in the form of phosphate fertilizers is immobilized rapidly and becomes unavailable to plants. To improve the plant growth and yield and to minimize P loss from soils, the ability of a few soil microorganisms converting insoluble forms into soluble forms for phosphorus is an important trait in several plant growth-promoting microorganisms belonging to the genera Bacillus and Pseudomonas and the fungi belonging to the genera Penicillium and Aspergillus in managing soil phosphorus. The principal mechanism of solubilization of mineral phosphate by phosphate solubilizing bacteria (PSB) is the release of low molecular weight organic acids such as formic, acetic, propionic, lactic, glycolic, fumaric, and succinic acids and acidic phosphatases like phytase synthesized by soil microorganisms in soil. Hydroxyl and carboxyl groups from the organic acids can chelate the cations bound to phosphate, thereby converting it into soluble forms.

키워드

참고문헌

  1. Ahmad A.K., J. Ghulam, S.A. Mohammad, M.S.N. Syed, and R. Mohammad. 2009. Phosphorus Solubilizing Bacteria: Occurrence, Mechanisms and their Role in Crop Production. J. Agric. Biol. Sci. 1:48-58.
  2. Alan, E.R. and J.S. Richard. 2011. Soil microorganisms mediating phosphorus availability. Plant Physiol. 156:989-996. https://doi.org/10.1104/pp.111.175448
  3. Asea, P.E.A., R.M.N. Kucey, and J.W.B. Stewart. 1988. Inorganic phosphate solubilization by two Penicillium species in solution culture. Soil Biol. Biochem. 20:459-464. https://doi.org/10.1016/0038-0717(88)90058-2
  4. Babu-Khan, S., T.C. Yeo, W.L. Martin, M.R. Duron, R.D. Rogers, and A.H. Goldstein. 1995. Cloning of a mineral phosphate-solubilizing gene from Pseudomonas cepacia. Appl. Environ. Microbiol. 61:972-978.
  5. Banik, S. and B.K. Dey. 1982. Available phosphate content of an alluvial soil as influenced by inoculation of some isolated phosphate solubilizing microorganisms. Plant Soil. 69:353-364. https://doi.org/10.1007/BF02372456
  6. Beech, I.B., M. Paiva., M. Caus., and C. Coutinho. 2001. Enzymatic activity and within biofilms of sulphate-reducing bacteria. In: P. G. Gilbert, D. Allison, M. Brading, J. Verran and J. Walker (eds.), Biofilm Community Interactions: chance or necessity? BioLine, Cardiff, UK. pp.231-239.
  7. Cosgrove, D.J. 1967. Metabolism of organic phosphates in soil. In: A.D. Mclaren and G.H. Peterson (eds.), Soil Biochemistry, Vol. I. Marcel & Dekker, NY. p.216-228.
  8. Dodor, D.E. and A.M. Tabatabai. 2003. Effect of cropping systems on phosphatases in soils. J. Plant Nutr. Soil Sci. 166:7-3. https://doi.org/10.1002/jpln.200390016
  9. Goldstein, A.H. and S.T. Liu. 1987. Molecular cloning and regulation of a mineral phosphate solubilizing gene from Erwinia herbicola. Bio/Technology. 5:72-74. https://doi.org/10.1038/nbt0187-72
  10. Goldstein, A.H. 1994. Involvement of the quinoprotein glucose dehydrogenase in the solubilization of exogenous phosphates by gram-negative bacteria. In: Torriani-Gorini, A., Yagil, E., and Silver, S. editors. Phosphate in Microorganisms: Cellular and Molecular Biology. Washington, DC: ASM Press. 197-203.
  11. Goldstein, A.H. 1995. Recent progress in understanding the molecular genetics and biochemistry of calcium phosphate solubilization by Gram-negative bacteria. Biol. Agri. Hort. 12:185-193. https://doi.org/10.1080/01448765.1995.9754736
  12. Goosen, N., H.P. Horsman, R.G. Huinen, and P. van de Putte. 1989. Acinetobacter calcoaceticus genes involved in biosynthesis of the coenzyme pyrrolo-quinoline-quinone: nucleotide sequence and expression in Escherichia oli K-12. J. Bacteriol. 171:447-455.
  13. Gyaneshwar, P., L.J. Parekh, G. Archana, P.S. Podle, M.D. Collins, R.A. Hutson, and K.G. Naresh. 1999. Involvement of a phosphate starvation inducible glucose dehydrogenase in soil phosphate solubilization by Enterobacter asburiae. FEMS Microbiol. Lett. 171:223-229. https://doi.org/10.1111/j.1574-6968.1999.tb13436.x
  14. Halder, A.K., A.K. Mishra, P. Bhattacharyya, P. and P.K. Chakrabartty. 1990. Solubilization of rock phosphate by Rhizobium and Bradyrhizobium. J Gen. Appl. Microbiol. 36:81-92. https://doi.org/10.2323/jgam.36.81
  15. Hayat, R., S. Ali, U. Amara, R. Khalid, and I. Ahmed. 2010. Soil beneficial bacteria and their role in plant growth promotion: a review. Ann Microbiol. 60:579-98. https://doi.org/10.1007/s13213-010-0117-1
  16. Hinsinger, P. 2001. Bioavailability of soil inorganic P in the rhizosphere as affected by root induced chemical changes: a review. Plant Soil 237:173-195. https://doi.org/10.1023/A:1013351617532
  17. Illmer, P. and F. Schinner. 1992. Solubilization of inorganic phosphates by microorganisms isolated from forest soil. Soil Biol. Biochem. 24:389-395. https://doi.org/10.1016/0038-0717(92)90199-8
  18. Jansson, M. 1987. Anaerobic dissolution of iron-phosphorus complexes in sediment due to the activity of nitratereducing bacteria, Microb. Ecol. 14:81-89. https://doi.org/10.1007/BF02011573
  19. Joa, J.H., H.C. Lim, S.G. Han, S.J. Chun, and J.S. Suh. 2007. Characteristics of Bacillus sphaericus PSB-13 as phosphate solubilizing bacterium isolated from citrus orchard soil. Korean J. Soil Sci. Fert. 40:405-411.
  20. Kang, S.C. and M.C. Choi. 1999. Solid culture of phosphate-solubilizing fungus, Penicillium sp. PS-113. Kor. J. Appl. Microbiol. Biotechnol. 27:1-7.
  21. Kang, S.C., M.O. Kang, and U.H. Yang. 2001. Mechanism of free phosphate production by penicillium sp. GL-101, phosphate solubilizing fungus, in the submerged culture. Korean J. Environ. Agric. 20:1-7.
  22. Kim, H.O., Z.K. Uo, S.C. Lee, and R.M.N. Kucey. 1984. Mycorrhizae distribution and rock phosphate dissolution by soil fungi in the citrus fields in Jeju-do. Cheju National University Journal. 17:45-50.
  23. Kim, J.M. and K.H. Kim. 2006. Nutrient removal ability by phosphate solubilizing bacteria and effect on crop growth. Exhibition in National Science Fair. [in Korean]
  24. Kim, K.H. 2006. Soil Science. Hyangmoonsa. p.313. [n Korean]
  25. Kim, K.Y., D. Jordan D. and G.A. McDonald. 1997. Solubilization of hydroxyapatite by Enterobacter agglomerans and cloned Escherichia coli in culture medium, Biol. Fert. Soils 24:347-352. https://doi.org/10.1007/s003740050256
  26. Kim, K.Y., D. Jordan and G.A. McDonald. 1998. Effect of phosphate-solubilizing bacteria and vesicular-arbuscular mycorrhizae on tomato growth and soil microbial activity. Biol. Fert. Soils. 26:79-87.
  27. Kpomblekou, K. and M.A. Tabatabai. 1994. Effect of organic acids on release of phosphorus from phosphate rocks. Soil Sci. 158:442-453. https://doi.org/10.1097/00010694-199415860-00006
  28. Kucey, R.M.N. 1988. Effect of penicillium bilaji on the solubility and uptake of P and Micronutrients from soil by wheat. Can. J. Soil. Sci. 68:261-270. https://doi.org/10.4141/cjss88-026
  29. Kuenen, J.G. and W.N. Konigns. 1987. Energy transduction by electron transfer via a pyrrolo-quinoline quinone-dependent glucose dehydrogenase in Escherichia coli, Pseudomonas putida, and Acinetobacter calcoaceticus (var. lwoffii). J Bacteriol. 163:493-499.
  30. Lee, C.W., Y.J. Jung, K.A. Lee, S.L. Choi, Y.G. Kim, and Y.L. Choi. 2004. Isolation and characteristic of the phosphate solubilizing bacteria Klebsiella sp. DA 71-1. J. Life Sci. 14:174-179. https://doi.org/10.5352/JLS.2004.14.1.174
  31. Liu, T.S., L.Y. Lee, C.Y. Tai, C.H. Hung, Y.S. Chang, J.H. Wolfram, R. Rogers, and A.H. Goldstein. 1992. Cloning of an Erwinia herbicola gene necessary for gluconic acid production and enhanced mineral phosphate solubilization in Escherichia coli HB101: Nucleotide sequence and probable involvement in biosynthesis of the coenzyme pyrroloquinoline quinone. J. Bacteriol. 174: 5814-5819.
  32. Mikanova, O., J. Kubat, T. Simon, K. Vorisek, and D. Randova. 1997. Influence of soluble phosphate on P-solubilizing activity of bacteria. Rostlinna-Vyroba-UZPI. 43:421-424.
  33. Molla, M.A.Z., A.A. Chowdhury, A. Islam, and S. Hoque. 1984. Microbial mineralization of organic phosphate in soil. Plant Soil. 78:393-399. https://doi.org/10.1007/BF02450372
  34. Nahas, E. 1996. Factors determining rock phosphate solubilization by microorganism isolated from soil. World J. Microb. Biotechnol. 12:18-23.
  35. Nautiyal, C.S. 1999. An efficient microbiological growth medium for screening phosphate solubilizing microorganisms. FEMS Microbiology Letters. 170:265-270. https://doi.org/10.1111/j.1574-6968.1999.tb13383.x
  36. Oh, S.H. and C.S. Lee. 1997. Saving soil and method of fertilization. National Agricultural Cooperative Federation. p.388. [in Korean]
  37. Park, B.K., J.H. Na, B.H. Hwang, I.J. Lee, K.Y. Kim, and Y.W. Kim. 2005. Effect of phosphate bio fertilizer produced by Enterobacter intermedium on rhizosphere soil properties and lettuce growth. Korean J. Soil Sci. Fert. Vol. 38(1):15-24.
  38. Park, J.S. 1992. Crop Physiology. Hyangmoonsa. p.437. [in Korean]
  39. Paul, E.A. and Clark, F.E. 1989. Soil Microbiology and Biochemistry. Academic press, New York, USA.
  40. Perez, E., M. Sulbaran, M.M. Ball, and L.A. Yarzabal. 2007. Isolation and characterization of mineral phosphatesolubilizing bacteria naturally colonizing a limonitic crust in the south-eastern Venezuelan region. Soil Biol. Biochem. 39: 2905-2914. https://doi.org/10.1016/j.soilbio.2007.06.017
  41. Richardson, A.E., J.M. Barea, A.M. McNeill, and C. Prigent-Combaret. 2009. Acquisition of phosphorus and nitrogen in the rhizosphere and plant growth promotion by microorganisms. Plant Soil. 321:305-39 https://doi.org/10.1007/s11104-009-9895-2
  42. Rodriguez, H. and R. Fraga. 1999. Phosphate solubilizing bacteria and their role in plant growth promotion. Biotechnol. Adv. 17:319-339. https://doi.org/10.1016/S0734-9750(99)00014-2
  43. Rural Development Administration. 2000. Technical development and practical strategy of environmentally-friendly agriculture. p.368. [in Korean]
  44. Ryan P.R., E. Delhaize, and D.L. Jones. 2001. Function and mechanism of organic anion exudation from plant roots. Annu. Rev. Plant Physiol. Plant Mol. Biol. 52:527-560. https://doi.org/10.1146/annurev.arplant.52.1.527
  45. Sagoe, C.I., T. Ando, K. Kouno and T. Nagaoka. 1998. Relative importance of protons and solution calcium concentration in phosphate rock dissolution by organic acids. Soil Sci. Plant Nutr. 44:617-625. https://doi.org/10.1080/00380768.1998.10414485
  46. Salih, H.M., A.I. Yonka, A.M. Abdul-Rahem, and B.H. Munam. 1989. Availability of phosphorus in calcareous soil treated with rock phosphate or superphosphate as affected by phosphate dissolving fungi. Plant and Soil. 120:181-185. https://doi.org/10.1007/BF02377067
  47. Van Schie, B.J., K.J. Hellingwerf, J.P. van Dijken, M.G.L. Elferink, J.M. van Dijl, Z.K. Uo, H.O. Kim, and S.C. Lee. 1985. Improvement of rock phosphate utilization efficiency-Distribution of V. A. mycorrhizae on Cheju island, and isolation and cultivation of rock phosphate solubilizing fungi. Cheju National University Journal. 20:81-92.
  48. Seeling, B. and R.J. Zasoski. 1993. Microbial effects in maintaining organic and inorganic solution phosphorus concentrations in a grassland topsoil. Plant Soil. 148:277-284. https://doi.org/10.1007/BF00012865
  49. Son, H.J., Y.G. Kim, and S.J. Lee. 2003. Isolation, identification and physiological characteristics of biofertilizer resources, insoluble phosphate-solubilizing bacteria. Korean J. Microbiol. 39(1):51-55.
  50. Suh, J.S., S.K. Lee, K.S. Kim, and K.Y. Seong. 1995. Solubilization of insoluble phosphates by Pseudomonas putida, Penicillium sp. and Aspergillus niger isolated from Korean soils. J. Korean Soc. Soil Sci. Fert. 28:278-286.
  51. Suh, J.S. 1994. Study of microbiological use of soil accumulated accumulated phosphorus by refractory phosphate solubilizing bacteria. Chonnam National University. [in Korean]
  52. Suh, J.S. and J.S. Kwon. 2008. Characterization of phosphatesolubilizing microorganisms in upland and plastic film house soils. Korean J. Soil Sci. Fert. 41(5):348-353.
  53. Suh, J.S. and J.S. Kwon. 2004. Evaluation of nutrient cycling function and application of the phosphate solubilizing microbes. Research of agricultural environment 2004. 911-937.
  54. Surange, S., A.G. Wollum, N. Kumar and C.S. Nautiyal. 1995. Characterization of Rhizobium from root nodules of leguminous trees growing in alkaline soils. Can. J. Microbiol. 43:891-894.
  55. Tao, G.C., S.J. Tian, M.Y. Cai, and G.H. Xie. 2008. Phosphate-solubilizing and mineralizing abilities of bacteria isolated from soils. Pedosphere. 18(4):515-523. https://doi.org/10.1016/S1002-0160(08)60042-9
  56. Tarafdar, J.C. and N. Claasen. 1988. Organic phosphorus compounds as a phosphorus source for higher plants through the activity of phosphatases produced by plant roots and microorganisms. Biol. Fert. Soils 5:308-312.
  57. Ryan P.R., E. Delhaize, and D.L. Jones. 2001. Function and mechanism of organic anion exudation from plant roots. Annu. Rev. Plant Physiol. Plant Mol. Biol. 52:527-560. https://doi.org/10.1146/annurev.arplant.52.1.527
  58. Vance, C.P., C. Uhde-Stone, and D.L. Allan. 2003. Phosphorus acquisition and use: critical adaptations by plants for securing a nonrenewable resource. New Phytol. 157:423-47. https://doi.org/10.1046/j.1469-8137.2003.00695.x
  59. Varsha, N. and H.H. Patel. 2000. Aspergillus aculeatus as a rock phosphate solubilizer, Soil Biol. Biochem. 32:559-565. https://doi.org/10.1016/S0038-0717(99)00184-4
  60. Vassilev, N., M. Toro, M. Vassileva, R. Azcon, and J.M. Barea. 1997. Rock phosphates solubilization by immobilized cells of Enterobacter sp. in fermentation and soil conditions. Bioresour. Technolo. 61:29-32. https://doi.org/10.1016/S0960-8524(97)84694-9
  61. Vassilev, N. and M. Vassilev. 2003. Biotechnological solubilization of rock phosphate on media containing agro-industrial wastes. Appl. Microbiol. Biotech. 61:435-440. https://doi.org/10.1007/s00253-003-1318-3
  62. Whitelaw, M.A. 2000. Growth promotion of plants inoculated with phosphate solubilizing fungi. Adv. Agron. 69:99-151.
  63. Wu, S.C., Z.H. Cao, Z.G. Li, K.C. Cheung, and M.H. Wong. 2005. Effects of biofertilizer containing N-fixer, P and K solubilizers and AM fungi on maize growth: a greenhouse trial. Geoderma. 125:155-166. https://doi.org/10.1016/j.geoderma.2004.07.003
  64. Yadaf, R.S. and J.C. Tarafdar. 2001. Influence of organic and inorganic phosphorus supply on the maximum secretion of acid phosphatase by plants. Biol. Fert. Soils. 34:140-143. https://doi.org/10.1007/s003740100376

피인용 문헌

  1. Soil Chemical Property and Leaf Mineral Nutrient of Ginseng Cultivated in Paddy Field Occurring Leaf Discoloration vol.21, pp.4, 2013, https://doi.org/10.7783/KJMCS.2013.21.4.289
  2. Combined application of bio-organic phosphate and phosphorus solubilizing bacteria (Bacillus strain MWT 14) improve the performance of bread wheat with low fertilizer input under an arid climate vol.49, pp.15178382, 2018, https://doi.org/10.1016/j.bjm.2017.11.005