Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
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Effect of Sargassum micracanthum extract on Lipid Accumulation and Reactive Oxygen Species (ROS) Production during Differentiation of 3T3-L1 Preadipocytes

3T3-L1 세포분화 중 지방축적 및 ROS 생성에 대한 잔가시 모자반 추출물의 효과

  • Lee, Young-Jun (Department of Food Science and Biotechnology, Kangwon National University) ;
  • Yoon, Bo-Ra (Department of Food Science and Biotechnology, Kangwon National University) ;
  • Choi, Hyeon-Son (Department of Food Science and Biotechnology, CHA University) ;
  • Lee, Boo-Yong (Department of Food Science and Biotechnology, CHA University) ;
  • Lee, Ok-Hwan (Department of Food Science and Biotechnology, Kangwon National University)
  • 이영준 (강원대학교 식품생명공학과) ;
  • 윤보라 (강원대학교 식품생명공학과) ;
  • 최현선 (차의과학대학교 식품생명공학과) ;
  • 이부용 (차의과학대학교 식품생명공학과) ;
  • 이옥환 (강원대학교 식품생명공학과)
  • Received : 2012.02.01
  • Accepted : 2012.04.06
  • Published : 2012.06.30
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Abstract

Obesity, a strong risk factor for the development of chronic diseases, is characterized by an increase in the number and size of adipocytes differentiated from precursor cells, preadipocytes. Recent research suggests that increased reactive oxygen species (ROS) production in 3T3-L1 adipocyte facilitates adipocyte differentiation and fat accumulation. This study was to investigate whether reduced ROS production by Sargassum micracanthum extract (SME) could protect the development of obesity through inhibition of adipogenesis. 3T3-L1 preadipocytes were treated SME for up to 8 days following standard induction of differentiation. The extent of differentiation reflected by amount of lipid accumulation and ROS production was determined by Oil red O staining and nitroblue tetrazolium (NBT) assay. Treatment of SME significantly inhibited ROS production and adipocyte differentiation that is depend on down regulation of NADPH oxidase 4 (NOX4), a major ROS generator, and peroxisome proliferator-activated receptor gamma ($PPAR{\gamma}$) and CCAAT/enhancer-binding protein alpha ($C/EBP{\alpha}$), a key adipogenic transcription factor. These results indicate that SME can inhibit adipogenesis through a reduced ROS level that involves down-regulation of NOX4 expression or via modulation of adipogenic transcription factor.

본 연구에서는 잔가시 모자반 추출물의 항비만 및 항산화 효과를 연구하기 위하여 3T3-L1 전지방세포에 분화 유도물질을 처리하여 분화 과정 중에 잔가시 모자반의 지방축적과 ROS 생성 억제 효과를 관찰하였다. 잔가시 모자반 추출물은 XTT assay에서 두 농도(10 및 100 ${\mu}g/mL$) 모두에서 세포 독성을 보이지 않았다. 지방세포 분화 중 세포 내 지방축적 및 ROS 생성량을 비교한 결과, 잔가시 모자반 추출물을 처리한 지방세포의 경우 지방축적량과 ROS 생성량 모두 유의적으로 억제되는 것으로 나타났다. 특히 잔가시 모자반 추출물을 처리함으로써 지방세포 분화와 관련된 전사인자인 $PPAR{\gamma}$와 $C/EBP{\alpha}$ 발현을 유의적으로 감소시켰으며, ROS의 생성과 관련이 있는 주요 효소인 NOX4의 발현 또한 유의적으로 감소하였다. 이 결과를 통해 잔가시 모자반 추출물이 3T3-L1 지방세포 내 중성지방의 축적 억제 효과와 더불어 ROS 생성 억제에 효과적으로 작용함을 확인하였다. 따라서 잔가시 모자반은 비만과 같이 대사증후군 관련 질환의 개선을 위한 천연물 기능성 소재로의 활용이 기대된다.

Keywords

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