DOI QR코드

DOI QR Code

Effect of Addition Level of Green Tea Extract on the Lactic Acid Bacteria, Oxidative Stability, and Aroma in Kimchi-fermented Sausage

녹차 추출물의 첨가 수준이 김치 발효 소시지의 젖산균, 산화안정성 및 향기에 미치는 영향

  • Kang, Sun-Moon (National Institute of Animal Science, Rural Development Administration) ;
  • Kim, Tae-Sil (Department of Animal Products and Food Science, Kangwon National University) ;
  • Song, Young-Han (Department of Animal Life System, Kangwon National University) ;
  • Kwon, Il-Kyoung (Department of Animal Products and Food Science, Kangwon National University) ;
  • Cho, Soo-Hyun (National Institute of Animal Science, Rural Development Administration) ;
  • Park, Beom-Young (National Institute of Animal Science, Rural Development Administration) ;
  • Lee, Sung-Ki (Department of Animal Products and Food Science, Kangwon National University)
  • 강선문 (농촌진흥청 국립축산과학원) ;
  • 김태실 (강원대학교 동물식품응용과학과) ;
  • 송영한 (강원대학교 동물생명시스템학과) ;
  • 권일경 (강원대학교 동물식품응용과학과) ;
  • 조수현 (농촌진흥청 국립축산과학원) ;
  • 박범영 (농촌진흥청 국립축산과학원) ;
  • 이성기 (강원대학교 동물식품응용과학과)
  • Received : 2012.05.22
  • Accepted : 2012.06.12
  • Published : 2012.08.31

Abstract

This study was carried out to investigate the effect of the addition level (0 ppm, 400 ppm, 800 ppm, and 1,200 ppm) of green tea extract on the lactic acid bacteria, oxidative stability, and aroma in kimchi-fermented sausage. The sample sausages were fermented at $24^{\circ}C/RH$ 89% until attained to a pH value of 4.9 (for 17 h), and then dried at $10^{\circ}C/RH$ 75-80% for 6 d. The lactic acid bacteria count and pH value were 7.5-7.7 Log CFU/g sausage and 4.30-4.33, respectively, at 6 d of ripening. The results of those did not show significant differences among all treatments. The formation of TBARS (2-thiobarbituric acid reactive substances) was significantly lowered by increasing the addition level of green tea extract. During ripening periods, the CIE $L^*$ and $a^*$ values decreased; however, the $b^*$ value increased due to the addition of green tea extract. Utilizing an electric nose, the aroma pattern was clearly discriminated between green tea extract treatments and the control. Therefore, in kimchi-fermented sausages, the high addition level of green tea extract improved the lipid oxidation stability. In addition, regardless of the addition level, green tea extract changed the aroma while reducing the color stability. Moreover, it did not have any effect on the growth of lactic acid bacteria.

본 연구는 녹차 추출물의 첨가 수준(0 ppm, 400 ppm, 800 ppm 및 1,200 ppm)이 김치 발효 소시지의 젖산균, 산화안정성 및 향기에 미치는 영향을 구명하고자 실시하였다. 시료는 $24^{\circ}C/RH$ 89%에서 pH 4.9까지(17시간) 발효시킨 후 $10^{\circ}C/RH$ 75-80%에서 6일간 건조시켰다. 젖산균수와 pH는 최종 6일째에 각각 7.5-7.7 Log CFU/g sausage 및 4.30-4.33이었으며, 녹차 추출물의 첨가 수준에 따른 차이가 없었다. TBARS(2-thiobarbituric acid reactive substances) 함량은 첨가 수준이 증가함에 따라 현저하게 억제되었다. 명도($L^*$)와 적색도($a^*$)는 녹차 추출물의 첨가에 의해 숙성 중 감소되었으며, 황색도($b^*$)는 증가되었다. 전자코(electronic nose)에 의한 향기 패턴은 첨가구들과 대조구간에 뚜렷한 차이를 보였다. 따라서 녹차 추출물의 첨가 수준의 증가는 김치 발효 소시지의 지방산화 안정성을 향상시켰다. 또한 첨가 수준과 관계 없이 녹차 추출물은 색깔 안정성을 저하시키고, 젖산균의 생장에 영향을 미치지 못하였으나, 향기를 변화시켰다.

Keywords

References

  1. Ahn, B. W., Lee, D. H., Yeo, S. G., Kang, J. H., Do, J. R., Kim, S. B., and Park, Y. H. (1993) Inhibitory action of natural food components on the formation of carcinogenic nitrosamine. Bull. Korean Fish. Soc. 26, 298-304.
  2. Baydar, H., Sagdic, O., Gülcan, Ö., and Karadogan, T. (2004) Antibacterial activity and composition of essential oils from Origanum, Thymbra, and Satureja species with commercial importance in Turkey. Food Control 15, 169-172. https://doi.org/10.1016/S0956-7135(03)00028-8
  3. Bozkurt, H. (2006) Utilization of natural antioxidants: green tea extract and Thymbra spicata oil in Turkish dry-fermentedsausage. Meat Sci. 73, 442-450. https://doi.org/10.1016/j.meatsci.2006.01.005
  4. Branen, A. L. (1975) Toxicology and biochemistry of butylated hydroxyl anisole and butylated hydroxyl toluene. J. Am. Oil Chem. Soc. 52, 59-63. https://doi.org/10.1007/BF02901825
  5. Choi, S. M., Jeon, Y. S., Rhee, S. H., and Park, K. Y. (2002) Red pepper powder and Kimchi reduce body weight and blood/tissue lipid in rats fed a high fat diet. Nutraceu. Food 7, 162-167. https://doi.org/10.3746/jfn.2002.7.2.162
  6. Collins, M. D. and Gibson, G. R. (1999) Probiotics, prebiotics, and synbiotics: approaches for modulating the microbial ecology of the gut. Am. J. Clin. Nutr. 69, 1052S-1057S.
  7. Comi, G., Urso, R., Iacumin, L., Rantsiou, K., Cattaneo, P., Cantoni, C., and Cocolin, L. (2005) Characterisation of naturally fermented sausages produced in the North East of Italy. Meat Sci. 69, 381-392. https://doi.org/10.1016/j.meatsci.2004.08.007
  8. Degenhart, J. (1988) Technologia de produtos curados. In: $7^{\circ}$ycurso de technologia da carne. ITAL, Campinas, Brazil, pp. 51-71.
  9. Fujiwara, M. (1972) Antihypercholesterolemic effect of sulfur containing amino acid, S-methyl-L-cysteine sulfoxide isolated from cabbage. Experimenta 28, 254-259 https://doi.org/10.1007/BF01928671
  10. Halliwell, B. and Gutteridge, J. M. C. (1986) Oxygen free radicals and iron in relation to biology and medicine: some problems and concepts. Arch. Biochem. Biophys. 246, 501-514. https://doi.org/10.1016/0003-9861(86)90305-X
  11. Hampikyan, H. and Ugur, M. (2007) The effect of nisin on L. monocytogenes in Turkish fermented sausages (sucuks). Meat Sci. 76, 327-332. https://doi.org/10.1016/j.meatsci.2006.11.014
  12. Hayes, J. E., Stepanyan, V., Allen, P., O'Grady, M. N., and Kerry, J. P. (2010) Effect of lutein, sesamol, ellagic acid and olive leaf extract on the quality and shelf-life stability of packaged raw minced beef patties. Meat Sci. 84, 613-620. https://doi.org/10.1016/j.meatsci.2009.10.020
  13. Hugas, M. and Monfort, J. M. (1997) Bacterial starter cultures for meat fermentation. Food Chem. 59, 547-554. https://doi.org/10.1016/S0308-8146(97)00005-8
  14. Jung, D. W., Nam, E. S., and Park, S. I. (2005) Effect of green tea powder on growth of lactic culture. Korean J. Food Nutr. 18, 325-333.
  15. Kanner, J., Harel, S., and Jaffe, R. (1991) Lipid peroxidation of muscle food as affected by NaCl. J. Agr. Food Chem. 39, 1017-1021. https://doi.org/10.1021/jf00006a002
  16. Kanner, J. (1994) Oxidative processes in meat and meat products: quality implications. Meat Sci. 36, 169-189. https://doi.org/10.1016/0309-1740(94)90040-X
  17. Kawada, T., Hagigara, K., and Iwai, K. (1986) Effect of capsaicin on lipid metabolism in rats fed diet. J. Nutr. 116, 1272-1279.
  18. Kim, K. H. (2002) Effect of addition methods of green tea on fermentation characteristics of Kimchi. Korean J. Food Preserv. 9, 406-410.
  19. Kim, S. Y., Kim, J. D., Son, J. S., Lee, S. K., Park, K. J., and Park, M. S. (2011) Biochemical and molecular identification of antibacterial lactic acid bacteria isolated from Kimchi. Korean J. Food Sci. Technol. 43, 446-452. https://doi.org/10.9721/KJFST.2011.43.4.446
  20. Klettner, P. G. and List, D. (1978) Lactic acid production during ripening of dry sausages to which starter cultures have been added. Fleischwirtschaft 58, 136-139.
  21. Ko, J. L., Oh, C. K., Oh, M. C., and Kim, S. H. (2009) Depletion of nitrite by lactic acid bacteria isolated from commercial Kimchi. J. Korean Soc. Food Sci. Nutr. 38, 892-901. https://doi.org/10.3746/jkfn.2009.38.7.892
  22. Kwon, D. Y., Koo, M. S., Ryoo, C. R., Kang, C. H., Min, K. H., and Kim, W. J. (2002) Bacteriocin produced by Pediococcus sp. in Kimchi and its characteristics. J. Microbiol. Biotech. 12, 96-105.
  23. Kwon, M. J., Song, Y. S., and Song, Y. O. (1998) Antioxidative effects of Kimchi ingredients on rabbits fed cholesterol diet. J. Korean Soc. Food Sci. Nutr. 27, 1189-1196.
  24. Lee, G. H., La, I. J., Cho, H. J., Yea, M. J., Kim, S. B., Park, J. Y., and Kim, S. H. (2009) Changes in quality characteristics of green tea beverage PET during high temperature storage. J. Korean Soc. Food Sci. Nutr. 38, 98-104. https://doi.org/10.3746/jkfn.2009.38.1.098
  25. Lee, J. Y. and Kunz, B. (2005) The antioxidant properties of Baechu-kimchi and freeze-dried Kimchi-powder in fermented sausages. Meat Sci. 69, 741-747. https://doi.org/10.1016/j.meatsci.2004.11.006
  26. Lee, J. Y., Kim, C. J., and Kunz, B. (2006) Identification of lactic acid bacteria isolated from Kimchi and studies on their suitability for application as starter culture in the production of fermented sausages. Meat Sci. 72, 437-445. https://doi.org/10.1016/j.meatsci.2005.08.013
  27. Lee, S. K., Yoo, I. J., Kim, Y. B., and Kim, K. S. (1990) Fermentation of sausage using Kimchi. Korean J. Anim. Sci. 32, 707-714.
  28. Lee, Y. M., Kwon, M. J., Kim, J. K., Suh, H. S., Choi, J. S., and Song, Y. O. (2004) Isolation and identification of active principle in Chinese cabbage Kimchi responsible for antioxidant effect. Korean J. Food Sci. Technol. 36, 129-133.
  29. Leistner, L. and Rodel, W. (1975) The significance of water activity for microorganisms in meats. In: Water relations of foods. Duckworth, R. B. (ed), Academic Press, London, pp. 309-323.
  30. Lim, C. R., Park, H. K., and Han, H. U. (1989) Reevaluation of isolation and identification of gram-positive bacteria in Kimchi. Korean J. Microbiol. 27, 404-414.
  31. Lucke, F. K. (1994) Fermented meat products. Food Res. Int. 27, 299-307. https://doi.org/10.1016/0963-9969(94)90098-1
  32. Lucke, F. K. (1998) Fermented sausages. In: Microbiology of fermented foods. Wood, B. J. B. (ed), Blackie Academic and Professional, NY, pp. 441-483.
  33. Manzocco, L., Anese, M., and Nicoli, M. C. (1998) Antioxidant properties of green tea extracts as affected by processing. Lebensm. -Wiss. -Technol. 31, 694-698. https://doi.org/10.1006/fstl.1998.0491
  34. Marco, A., Navarro, J. L., and Flores, M. (2006) The influence of nitrite and nitrate on microbial, chemical and sensory parameters of slow dry fermented sausage. Meat Sci. 73, 660-673. https://doi.org/10.1016/j.meatsci.2006.03.011
  35. Nassu, R. T., Gonalves, L. A. G., Pereira da Silva, M. A. A., and Beserra, F. J. (2003) Oxidative stability of fermented goat meat sausage with different levels of natural antioxidant. Meat Sci. 63, 43-49. https://doi.org/10.1016/S0309-1740(02)00051-7
  36. Noh, K. A., Kim, D. H., Choi, N. S., and Kim, S. H. (1999) Isolation of fibrinolytic enzyme producing strains from Kimchi. Korean J. Food Sci. Technol. 31, 219-223.
  37. Papamanoli, E., Tzanetakis, N., Litopoulou-Tzanetaki, E., and Kotzekidou, P. (2003) Characterization of lactic acid bacteria isolated from a Greek dry-fermented sausage in respect of their technological and probiotic properties. Meat Sci. 65, 859-867. https://doi.org/10.1016/S0309-1740(02)00292-9
  38. Park, H. K., Lim, C. R., and Han, H. U. (1990) Microbial succession in Kimchi fermentation at different temperature. Res. Inst. Basic Soc. Inha Univ. 11, 161-169.
  39. Park, K. R., Lee, S. G., Nam, T. G., Kim, Y. J., Kim, Y. R., and Kim, D. O. (2009) Comparative analysis of catechins and antioxidant capacity in various grades of organic green teas grown in Boseong, Korea. Korean J. Food Sci. Technol. 41, 82-86.
  40. Park, K. Y. and Cheigh, H. S. (2004) Kimchi. In: Handbook of vegetable preservation and processing. Hui, Y. H., Ghazala, S., Graham, D. M., Murrell, K. D., and Nip, W. K. (eds) Marcel Dekker, Inc., NY, pp. 189-222.
  41. Park, S. B., Han, B. K., Oh, Y. J., Lee, S. J., Cha, S. K., Park, Y. S., and Choi, H. J. (2012) Bioconversion of green tea extract using lactic acid bacteria. Food Eng. Prog. 16, 26-32.
  42. Pelser, W. M., Linssen, J. P. H., Legger, A., and Houben, J. H. (2007) Lipid oxidation in n-3 fatty acid enriched Dutch style fermented sausages. Meat Sci. 75, 1-11. https://doi.org/10.1016/j.meatsci.2006.06.007
  43. Peter, F. S. (1975) The toxicology of nitrate, nitrite and nnitroso compounds. J. Sci. Food Agr. 26, 1761-1770. https://doi.org/10.1002/jsfa.2740261119
  44. Schillinger, U. and Lücke, F. K. (1989) Einsatz von milchsäurebakterien als schutzkulturen bei fleischerzeugnissen. Fleischwirtschaft 69, 879-882.
  45. Sheo, H. J. and Seo, Y. S. (2004) The effects of dietary Chinese cabbage Kimchi juice on the lipid metabolism and body weight gain in rats fed high-calories-diet. J. Korean Soc. Food Sci. Nutr. 33, 91-100. https://doi.org/10.3746/jkfn.2004.33.1.091
  46. Shin, D. W., Kim, M. S., Han, J. S., Lim, D. K., and Bak, W. S. (1996) Changes of chemical composition and microflora in commercial Kimchi. Korean J. Food Sci. Technol. 28, 137-145.
  47. Shin, Y. H., Oh, B. T., Choi, S. G., Heo, H. J., Lee, S. C., and Cho, S. H. (2009) Antimicrobial activity of an aqueous extract of green tea against food putrefactive microorganisms. Korean J. Food Preserv. 16, 392-399.
  48. Sinnhuber, R. O. and Yu, T. C. (1977) The 2-thiobarbituric acid reaction, an objective measure of the oxidative deterioration occurring in fats and oils. J. Jpn. Soc. Fish. Sci. 26, 259-267.
  49. Skibsted, L. H. (1992) Cured meat products and their oxidative stability. In: The chemistry of muscle-based foods. Johnston, D. E., Knight, M. K., and Ledward, D. A. (eds), The Royal Society of Chemistry, Cambridge, UK, pp. 266-286.
  50. So, M. H. and Kim, Y. B. (1995) Identification of psychrotrophic lactic acid bacteria isolated from Kimchi. Korean J. Food Sci. Technol. 27, 495-505.
  51. SPSS (2005) SPSS 14.0 for Windows Evaluation Version, SPSS Inc., Illinois, USA.
  52. Stacy, P. and William, S. H. (1993) Garlic supplementation and lipoprotein oxidation susceptibility. Lipids 28, 475-477. https://doi.org/10.1007/BF02535949
  53. Stahnke, L. H. (1995) Dried sausages fermented with Staphylococcus xylosus at different temperatures and with different ingredient levels-part I. Chemical and bacteriological data. Meat Sci. 41, 179-191. https://doi.org/10.1016/0309-1740(94)00070-N
  54. Toldra, F. (1992) The enzymology of dry-curing meat products. In: New technologies for meat and meat products. Smulders, F. J., Toldra, F., Flores, J., and Prieto, M. (eds), ECCEAMST/AUDET, Nijmegen, The Netherlands, pp. 209-231.
  55. Yang, C. S., Chung, J. Y., Yang, G., Chhabra, S. K., and Lee, M. J. (2000) Tea and tea polyphenols in cancer prevention. J. Nutr. 130, 472-478.
  56. Yanishlieva, N. V. and Marinova, E. M. (2001) Stabilisation of edible oils with natural antioxidants. Eur. J. Lipid Sci. Technol. 103, 752-767. https://doi.org/10.1002/1438-9312(200111)103:11<752::AID-EJLT752>3.0.CO;2-0
  57. Yu, M. H., Im, H. G., Im, N. K., Hwang, E. Y., Choi, J. H., Lee, E. J., Kim, J. B., Lee, I. S., and Seo, H. J. (2009) Antihypertensive activities of Lactobacillus isolated from Kimchi. Korean J. Food Sci. Technol. 41, 428-434.
  58. Zanardi, E., Ghidini, S., Battaglia, A., and Chizzolini, R. (2004) Lipolysis and lipid oxidation in fermented sausages depending on different processing conditions and different antioxidants. Meat Sci. 66, 415-423. https://doi.org/10.1016/S0309-1740(03)00129-3

Cited by

  1. Effect of Adding Lactobacillus-Fermented Solution on Characteristics of Chicken Breast Meat vol.41, pp.2, 2014, https://doi.org/10.5536/KJPS.2014.41.2.127
  2. Study on Physicochemical and Sensory Properties of Fermented Sausages on the Domestic Market vol.33, pp.4, 2012, https://doi.org/10.9724/kfcs.2017.33.4.371
  3. Lactobacillus plantarum MKHA15와 Leuconostoc mesenteroides MKSR을 첨가한 발효 채소 주스의 특성 vol.25, pp.4, 2012, https://doi.org/10.14373/jkda.2019.25.4.281