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MONITORING OF REMINERALIZATION OF DECALCIFIED ENAMEL USING QUANTITATIVE LIGHT-INDUCED FLUORESCENCE-D

Quantitative light-induced fluorescence-D를 이용한 탈회 법랑질의 재석회화 감시

  • You, Yon-Sook (Department of Pediatric Dentistry, Dental College, Dankook University) ;
  • Kim, Jong-Soo (Department of Pediatric Dentistry, Dental College, Dankook University)
  • 류연숙 (단국대학교 치과대학 소아치과학교실) ;
  • 김종수 (단국대학교 치과대학 소아치과학교실)
  • Received : 2012.07.18
  • Accepted : 2012.08.08
  • Published : 2012.08.31

Abstract

The objective of this in vitro study was to monitor the amount of remineralization of decalcified enamel according to the number of fluoride varnish application using quantitative light-induced fluorescence-D and polarizing microscope. Artificial white lesion induced on the sound 72 teeth, $CavityShield^{TM}$ (Group I), $FluroDose^{TM}$ (Group II) and $Flor-Opal^{(R)}$ Varnish (Group III) were applied 1, 2 or 3 times every two weeks. The following results was obtained: 1. In group I, II and III, ${\Delta}L$ were increased. From regression analysis of ${\Delta}L$ by the number of application, the equation was y = 3.878x + 90.612 in group I, y = 3.133x + 37.168 in group II, and y = 3.509x + 82.322 in group III(p < 0.05). 2. In group I, II and III, ${\Delta}D$ were decreased. From regression analysis of ${\Delta}D$ by the number of application, the equation was y = -2.336x + 107.235 in group I, y = -2.158x + 101.620 in group II, and y = -1.940x + 94.806 in group III(p < 0.05). 3. The Pearson correlation value between the ${\Delta}L$ and ${\Delta}D$ was -0.673 in group I, -0.574 in group II, and -0.431 in group III(p < 0.05).

인공 우식 용액으로 72개의 건전한 치아에 백색 병소를 형성을 유도하고 $CavityShield^{TM}$(Group I), $FluroDose^{TM}$(Group II) 그리고 $Flor-Opal^{(R)}$ Varnish(Group III)를 2주 간격으로 1, 2, 3회 도포하였다. 마지막 불소 바니쉬 도포 2주 후 QLF-D 영상을 촬영하여 광도 변화량(${\Delta}L$)을 측정하였고, 편광 현미경으로 법랑질 탈회 깊이 변화량(${\Delta}D$)을 측정하여 통계적으로 비교하였다. 1. Group I, II, III에서 QLF-D로 관찰한 결과, 1회 도포군보다 2주 간격으로 2, 3회 도포군에서 유의하게 ${\Delta}L$ 값이 증가하였고, 각 불소 바니쉬에서 도포 횟수에 따라 회귀 분석 결과, y = 3.878x + 90.612, y = 3.133x + 37.168, y = 3.509x + 82.322의 회귀 방정식이 산출되었다(p < 0.05). 2. Group I, II, III에서 편광 현미경으로 관찰한 결과, 1회 도포군보다 2주 간격으로 2, 3회 도포군에서 유의하게 ${\Delta}D$ 값이 감소하였고, 각 불소 바니쉬에서 도포 횟수에 따라 회귀 분석 결과, y = -2.336x + 107.235, y = -2.158x + 101.620, y = -1.940x + 94.806의 회귀 방정식이 산출되었다(p < 0.05). 3. 불소 바니쉬 적용하고 편광 현미경으로 관찰한 인공 우식의 깊이 변화량과 QLF-D로 관찰한 광도 변화량 사이의 피어슨 상관 계수는 Group I에서 -0.673, Group II에서 -0.574, 그리고 Group III에서 -0.431이었다(p < 0.05).

Keywords

References

  1. National institutes of health : Oral health in America. Rockville, US, 37-39, 2000.
  2. Ismail AI : Clinical diagnosis of precavitated carious lesions. Community Dent Oral Epidemiol, 25:13-23, 1997. https://doi.org/10.1111/j.1600-0528.1997.tb00895.x
  3. Feathersthone JDB : The Science and practice of caries prevention. JADA, 131:887-899, 2000.
  4. Stookey GK : Quantitative light fluorescence: A technology for early monitoring of the caries process. Dent Clin N Am, 49:753-770, 2005. https://doi.org/10.1016/j.cden.2005.05.009
  5. Wu J, Donly ZR, Donly KJ, Hackmyer S : Demineralization depth using QLF and a novel image processing software. Int J Dent, 1-7, 2010.
  6. Pitts NB : Modern concepts of caries measurement. Journal of dental restoration, 83:43-47, 2004. https://doi.org/10.1177/154405910408301S09
  7. Ismail AI, Hasson H : Fluoride supplements, dental caries and fluorosis - A systematic reiview. J Am Dent Assoc, 139:1457-1468, 2008. https://doi.org/10.14219/jada.archive.2008.0071
  8. Beltran-Aguilar ED, Goldstein JW, Lockwood SA : Fluoride varnishes. J Am Dent Assoc, 131:589-594, 2000. https://doi.org/10.14219/jada.archive.2000.0232
  9. Autio-Gold JT, Courts F : Assessing the effect of fluoride varnish on early enamel carious lesions in the primary dentition. J Am Dent Assoc, 132:1247-1253, 2001. https://doi.org/10.14219/jada.archive.2001.0367
  10. Yang J, Dutra V : Utility of radiology, laser fluorescence, and transillumination. Dent Clin N Am, 49: 739-752, 2005. https://doi.org/10.1016/j.cden.2005.05.010
  11. Nakata K, Nikaido T, Ikeda M, et al. : Relationship between fluorescence loss of QLF and depth of demineralization in an enamel erosion model. Dent Mater J, 28:523-529, 2009. https://doi.org/10.4012/dmj.28.523
  12. Hartles RL, Leaver AG : The fluorescence of teeth under ultraviolet irradiation. Biochem J, 54:632-638, 1953. https://doi.org/10.1042/bj0540632
  13. Angmar-Mansson B, Bosch JJ : Quantitative lightinduced fluorescence: a method for assessment of incipient caries lesions. Dentomaxillofac Radiol, 30: 298-307, 2001. https://doi.org/10.1038/sj.dmfr.4600644
  14. Park HJ, Kim JS, Yoo SH, Shin JS : Developing of QLF-D for early detection of dental caries. J Korean Acad Pediatr Dent, 38:317-326, 2011. https://doi.org/10.5933/JKAPD.2011.38.4.317
  15. Kim JY, Lee SH, Lee NY : Effect of fluoride in natural polymer on enamel demineralization. J Korean Acad Pediatr Dent, 37:35-43, 2010.
  16. Pretty IA, Pender N, Edger WM, Higham SM : The in vitro detection of early enamel de- and re-mineralization adjacent to bonded orthodontic cleats using quantitative light-induced fluorescence. Eur J Orthod, 25:217-223, 2003. https://doi.org/10.1093/ejo/25.3.217
  17. Adeyemi AA, Jarad FD, Jong EJ, et al. : The evaluation of a novel method comparing quantitative light-induced fluorescenec(QLF) with spectrophotometry to assess staining and bleaching of teeth. Clin Oral Invest, 14:19-25, 2010. https://doi.org/10.1007/s00784-009-0261-9
  18. Karlsson L, Lindgren LE, Trollsas K, et al. : Effect of supplementary amine fluoride gel in caries-active adolescents: A clinical QLF study. Acta Odontol Scand, 65:284-291, 2007. https://doi.org/10.1080/00016350701620644
  19. Higham SM, Pretty IA, Edgar WM, Smith PW : The use of in situ models and quantitative light-induced fluorescence(QLF) for the study of coronal caries. J Dent, 33:235-241, 2005. https://doi.org/10.1016/j.jdent.2004.10.016
  20. Pretty IA, Smith PW, Edgar WM, Higham SM : Detection on in vitro demineralization adjacent to restorations using quantitative light-induced fluorescence(QLF). Dent Mater, 19:368-374, 2003. https://doi.org/10.1016/S0109-5641(02)00079-9
  21. Twetman S, Petersson LG : Prediction of caries in pre-school children in relation to fluoride exposure. Eur J Oral Sci, 104:523-528, 1996. https://doi.org/10.1111/j.1600-0722.1996.tb00136.x
  22. Farhadian N, Miresmaeili A, Eslami B, Mehrabi S : Effect of fluoride varnish on enamel demineralization around bracket: An in-vivo study. Am J Orthod Dentofacial Orthop, 133:95-98, 2008. https://doi.org/10.1016/j.ajodo.2006.03.032
  23. Todd MA, Staley RN, Kanellis MJ, et al. : Effect of a fluoride vanish on deminerlization adjacent to orthodontic brackets. Am J Orthod Dentofacial Orthop, 116:159-167, 1999. https://doi.org/10.1016/S0889-5406(99)70213-1
  24. Ogaard B, Bosch JJ : Regression of white spot enamel lesions: A new optical method for quantitative longitudinal evaluation in vivo. Am J Orthod Dentofac Orthop, 106:238-242, 1994. https://doi.org/10.1016/S0889-5406(94)70042-7
  25. Al-Khateeb S, Oliveby A, Jong EJ, Angmar-Mansson B : Laser fluorescence quantification of remineralisation in situ of incipient enamel lesions: Influence of fluoride supplements. Caries Res, 31: 132-140, 1997. https://doi.org/10.1159/000262388
  26. Association of state and territorial dental directors fluoride committee : Fluoride varnish: an Evidence-based approach research brief. ASTDD, August:1-15, 2007.
  27. The American dental association Council on scientific affairs : Professionally applied topical fluoride: Executive summary of evidence-based clinical recommendations. ADA, May:1-3, 2006.
  28. Fluoride varnish by Alliance for a cavity-free future and Assessment service. Available from URL:www.allianceforacavityfreefuture.org(Assessd on August 8, 2012)
  29. Lee EH, Kim JS, Yoo SH : Remineralization effect of sodium fluoride varnish on white lesion by application intervals. J Korean Acad Pediatr Dent, 37:403-411, 2010.
  30. Vargas CM : Fluoride supplements prevent caries but can cause mild to moderate fluorosis. J Evid Base Dent Pract, 11:18-20, 2001.
  31. Penman AD, Brackin BT, Embrey R : Outbreak of acute fluoride poisoning caused by a fluoride overfeed, Mississippi, 1993. Public health reports, 112:403-409, 1997.
  32. Kaneshiro KP, Silva VE, Borro MF, et al. : Urinary fluoride output in children following the use of an dual-fluoride varnish formulation. J Appl Oral Sci, 17:179-183, 2009. https://doi.org/10.1590/S1678-77572009000300009

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