Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Antibacterial Activity and Protective Role against Gastric Cancer by Sedum sarmentosum

돌나물의 항균활성 및 위암예방효과

  • Choi, Ji Yeon (Department of Food Science and Nutrition, Pusan National University) ;
  • Kim, Hye Min (Department of Integrative Plant Science, Chung-Ang University) ;
  • Mok, So-Youn (Department of Integrative Plant Science, Chung-Ang University) ;
  • Choi, Kyung (Department of Forest Resource Conservation, Korea National Arboretum) ;
  • Ku, Jajung (Department of Forest Resource Conservation, Korea National Arboretum) ;
  • Park, Kwang-Woo (Department of Forest Resource Conservation, Korea National Arboretum) ;
  • Cho, Eun Ju (Department of Food Science and Nutrition, Pusan National University) ;
  • Lee, Sanghyun (Department of Integrative Plant Science, Chung-Ang University)
  • Received : 2012.04.30
  • Accepted : 2012.06.18
  • Published : 2012.09.30
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Abstract

The aim of this research was to investigate the industrial application of Sedum sarmentosum. Antibacterial activities of the n-hexane, methylene chloride (MC), ethyl acetate, and n-butanol fractions of S. sarmentosum were tested against Escherichia coli, Staphylococcus aureus, and Helicobacter pylori. The MC fraction showed the strongest antibacterial activity against E. coli, with an inhibition zone greater than 13 mm in disc assays. At $100{\mu}g/mL$, all fractions scavenged more than 50% of 1,1-diphenyl-2-picrylhydrazyl (DPPH) and hydroxyl radicals (${\cdot}OH$). In particular, the MC fraction showed the strongest scavenging activity against DPPH and ${\cdot}OH$. In addition, we found that treatment with the MC fraction inhibited the growth of H. pylori and gastric adenocarcinoma cells. The present results suggest that the MC fraction of S. sarmentosum would play the promising protective role against pathogenic bacteria and free radicals.

본 연구에서는 돌나물 분획물의 항산화 및 위암예방 효과를 다양한 균들과 위암세포주를 이용하여 검토하였다. Escherichia coli, Staphylococcus aureus 그리고 helicobacter pylori에 대한 돌나물의 n-hexane, methylene chloride (MC), ethyl acetate, n-butanol 분획물의 항균효과를 disc 확산법으로 알아본 결과, MC 분획물이 E. coli에 대해 13 mm의 억제존을 보여 가장 우수한 항균효과를 나타내는 것을 알 수 있었다. $100{\mu}g/mL$ 농도에서 모든 분획물은 1,1-diphenyl-2-picrylhydrazyl (DPPH)와 hydroxyl radicals (${\cdot}OH$)에서 50% 이상의 소거능을 나타냈다. 특히, MC 분획물이 DPPH와 ${\cdot}OH$에서 가장 높은 소거능을 보였다. 또한 MC 분획물은 H. pylori와 위암세포의 성장을 억제하는 것을 확인할 수 있었다. 그러므로 돌나물의 분획물 중 특히 MC 분획물이 radical 소거능, H. pylori에 대한 항균성 및 AGS 인체 위암세포 생존 저해효과가 우수한 활성물질을 함유하고 있을 것으로 생각된다.

Keywords

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