Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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Production System of Virus-free Apple Plants Using Heat Treatment and Shoot Tip Culture

열처리와 경정배양을 이용한 바이러스 무병 사과 생산 시스템

  • Lee, Gunsup (Fruit Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Kim, Jeong Hee (Apple Research Station, National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Kim, Hyun Ran (Future & Creation Strategy Team, Rural Development Administration) ;
  • Shin, Il Sheob (Fruit Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Cho, Kang Hee (Fruit Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Kim, Se Hee (Fruit Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Shin, Juhee (Fruit Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Kim, Dae Hyun (Fruit Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration)
  • 이건섭 (농촌진흥청 국립원예특작과학원 과수과) ;
  • 김정희 (농촌진흥청 국립원예특작과학원 사과시험장) ;
  • 김현란 (농촌진흥청 미래창조전략팀) ;
  • 신일섭 (농촌진흥청 국립원예특작과학원 과수과) ;
  • 조강희 (농촌진흥청 국립원예특작과학원 과수과) ;
  • 김세희 (농촌진흥청 국립원예특작과학원 과수과) ;
  • 신주희 (농촌진흥청 국립원예특작과학원 과수과) ;
  • 김대현 (농촌진흥청 국립원예특작과학원 과수과)
  • Received : 2013.11.12
  • Accepted : 2013.11.25
  • Published : 2013.12.31
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Abstract

In worldwide, viral diseases of apple plants has caused the serious problems like reduced production and malformation of fruits. Also, the damages of apple plants by virus and/or viroid infection (Apple chlorotic leaf spot virus, Apple stem grooving virus, Apple mosaic virus, and Apple scar skin viroid) were reported in Korea. However there is few report about the protection approach against the infection by apple viruses. Therefore, this paper introduced the experimental protocol for the development of virus-free apple cultivars (Danhong, Hongan, Saenara, Summerdream). Apple plants were treated at $37^{\circ}C$ for 4 weeks and shoot tips were cultured in vitro. After heat treatment, the detection of apple viruses was performed by RT-PCR using virusspecific detection primers in new apple cultivars. With the heat treatments followed by in vitro shoot tip culture, the proportion of virus-free stocks of 'Danhong', 'Hongan', 'Saenara', and 'Summerdream' was 28%, 16%, 12%, and 12%, respectively. Taken together, this approach can be a good tool for production of virus-free apple stocks.

국내외적으로 ACLSV, ASGV, ApMV, ASSVd와 같은 바이러스 및 바이로이드 병의 발생으로 사과 과실의 생산량 감소와 기형적인 외형 등 많은 문제점들이 보고되었다. 하지만 사과 바이러스의 감염에 대한 방제 대책은 거의 알려진 바가 없는 실정이다. 따라서 본 논문에서는 사과 신품종인 '단홍', '홍안', '새나라', '썸머드림'을 분양하기에 앞서 바이러스 무병묘를 생산하는 시스템을 확립하고자 하였다. $37^{\circ}C$가 유지되는 항온 항습장치에서 4주간 열처리를 하였으며 기내에서 경정 배양을 하였다. 열처리된 각각의 사과 신품종들은 바이러스 진단 프라이머를 통해 RT-PCR을 수행하여 바이러스 진단을 수행하였다. 결과적으로 '단홍'은 28%의 바이러스 무병묘를 확보할 수 있었으며 '홍안'은 16%, '새나라'와 '썸머드림'은 12%의 확률로 바이러스 무병 사과를 확보할 수 있었다. 본 연구결과는 열처리 및 경정배양을 통해 사과 신품종에서 바이러스 무병묘 생산 시스템 구축이 가능함을 보여주었다.

Keywords

References

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